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chromogenic alkaline phosphatase solution  (Vector Laboratories)


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    Vector Laboratories chromogenic alkaline phosphatase solution
    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
    Chromogenic Alkaline Phosphatase Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+substrate+chromogen+solution/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc04508498-84-1-5
    Average 96 stars, based on 1934 article reviews
    chromogenic alkaline phosphatase solution - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors"

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    Journal: Theranostics

    doi: 10.7150/thno.11711

    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
    Figure Legend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Techniques Used: Double Staining, Staining, Injection

    Related Articles

    Avidin-Biotin Assay:

    Article Title: Sex-related differences within sleep-wake dynamics, cataplexy, and EEG fast-delta power in a narcolepsy mouse model.
    Article Snippet: After incubation with primary antibody, the sections were rinsed for 3 × 10 min in TBS-T20 and incubated for 1 h in biotinylated donkey anti-rabbit secondary antibody (1:1000, #711-006-152, Jackson Immunolaboratories, UK). .. After washing for 3 × 10 min in TBS-T20, the sections were transferred to avidin-biotin complex solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) diluted in TBS-T20 according to manufacturer’s instructions for 1 h, rinsed in 1 × TBS-T20 and 2 × TBS for 10 min and incubated for 10 min in ImmPACT Vector Red Substrate chromogen solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) for MCH staining or in ImmPACT Vector BCIP/NBT Substrate chromogen solution (#SK-5400, Vector Laboratories, Burlingame, CA, USA) for HCRT staining with 1% 125 mM Levamisole (#31742, Sigma–Aldrich, Germany) to block endogenous alkaline phosphatase activity. .. After washing for 3 × 10 min in TBS, the sections were mounted on 0.5% gelatin (#24360.233, VWR chemicals, UK)/0.05% chrome alum (#A11816, Alfa Aesar, Germany)-coated superfrost glass slides (Hounisen, Aarhus, Denmark) and dried before subjected to a graded dehydration series using ethanol (70%, 96%, 99%) for 5 min per step, cleared in xylene for 10 min, and coverslipped with DPX new (#1.00579, Merck, Germany).

    Incubation:

    Article Title: Sex-related differences within sleep-wake dynamics, cataplexy, and EEG fast-delta power in a narcolepsy mouse model.
    Article Snippet: After incubation with primary antibody, the sections were rinsed for 3 × 10 min in TBS-T20 and incubated for 1 h in biotinylated donkey anti-rabbit secondary antibody (1:1000, #711-006-152, Jackson Immunolaboratories, UK). .. After washing for 3 × 10 min in TBS-T20, the sections were transferred to avidin-biotin complex solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) diluted in TBS-T20 according to manufacturer’s instructions for 1 h, rinsed in 1 × TBS-T20 and 2 × TBS for 10 min and incubated for 10 min in ImmPACT Vector Red Substrate chromogen solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) for MCH staining or in ImmPACT Vector BCIP/NBT Substrate chromogen solution (#SK-5400, Vector Laboratories, Burlingame, CA, USA) for HCRT staining with 1% 125 mM Levamisole (#31742, Sigma–Aldrich, Germany) to block endogenous alkaline phosphatase activity. .. After washing for 3 × 10 min in TBS, the sections were mounted on 0.5% gelatin (#24360.233, VWR chemicals, UK)/0.05% chrome alum (#A11816, Alfa Aesar, Germany)-coated superfrost glass slides (Hounisen, Aarhus, Denmark) and dried before subjected to a graded dehydration series using ethanol (70%, 96%, 99%) for 5 min per step, cleared in xylene for 10 min, and coverslipped with DPX new (#1.00579, Merck, Germany).

    Staining:

    Article Title: Sex-related differences within sleep-wake dynamics, cataplexy, and EEG fast-delta power in a narcolepsy mouse model.
    Article Snippet: After incubation with primary antibody, the sections were rinsed for 3 × 10 min in TBS-T20 and incubated for 1 h in biotinylated donkey anti-rabbit secondary antibody (1:1000, #711-006-152, Jackson Immunolaboratories, UK). .. After washing for 3 × 10 min in TBS-T20, the sections were transferred to avidin-biotin complex solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) diluted in TBS-T20 according to manufacturer’s instructions for 1 h, rinsed in 1 × TBS-T20 and 2 × TBS for 10 min and incubated for 10 min in ImmPACT Vector Red Substrate chromogen solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) for MCH staining or in ImmPACT Vector BCIP/NBT Substrate chromogen solution (#SK-5400, Vector Laboratories, Burlingame, CA, USA) for HCRT staining with 1% 125 mM Levamisole (#31742, Sigma–Aldrich, Germany) to block endogenous alkaline phosphatase activity. .. After washing for 3 × 10 min in TBS, the sections were mounted on 0.5% gelatin (#24360.233, VWR chemicals, UK)/0.05% chrome alum (#A11816, Alfa Aesar, Germany)-coated superfrost glass slides (Hounisen, Aarhus, Denmark) and dried before subjected to a graded dehydration series using ethanol (70%, 96%, 99%) for 5 min per step, cleared in xylene for 10 min, and coverslipped with DPX new (#1.00579, Merck, Germany).

    Blocking Assay:

    Article Title: Sex-related differences within sleep-wake dynamics, cataplexy, and EEG fast-delta power in a narcolepsy mouse model.
    Article Snippet: After incubation with primary antibody, the sections were rinsed for 3 × 10 min in TBS-T20 and incubated for 1 h in biotinylated donkey anti-rabbit secondary antibody (1:1000, #711-006-152, Jackson Immunolaboratories, UK). .. After washing for 3 × 10 min in TBS-T20, the sections were transferred to avidin-biotin complex solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) diluted in TBS-T20 according to manufacturer’s instructions for 1 h, rinsed in 1 × TBS-T20 and 2 × TBS for 10 min and incubated for 10 min in ImmPACT Vector Red Substrate chromogen solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) for MCH staining or in ImmPACT Vector BCIP/NBT Substrate chromogen solution (#SK-5400, Vector Laboratories, Burlingame, CA, USA) for HCRT staining with 1% 125 mM Levamisole (#31742, Sigma–Aldrich, Germany) to block endogenous alkaline phosphatase activity. .. After washing for 3 × 10 min in TBS, the sections were mounted on 0.5% gelatin (#24360.233, VWR chemicals, UK)/0.05% chrome alum (#A11816, Alfa Aesar, Germany)-coated superfrost glass slides (Hounisen, Aarhus, Denmark) and dried before subjected to a graded dehydration series using ethanol (70%, 96%, 99%) for 5 min per step, cleared in xylene for 10 min, and coverslipped with DPX new (#1.00579, Merck, Germany).

    Activity Assay:

    Article Title: Sex-related differences within sleep-wake dynamics, cataplexy, and EEG fast-delta power in a narcolepsy mouse model.
    Article Snippet: After incubation with primary antibody, the sections were rinsed for 3 × 10 min in TBS-T20 and incubated for 1 h in biotinylated donkey anti-rabbit secondary antibody (1:1000, #711-006-152, Jackson Immunolaboratories, UK). .. After washing for 3 × 10 min in TBS-T20, the sections were transferred to avidin-biotin complex solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) diluted in TBS-T20 according to manufacturer’s instructions for 1 h, rinsed in 1 × TBS-T20 and 2 × TBS for 10 min and incubated for 10 min in ImmPACT Vector Red Substrate chromogen solution (#AK-5000, Vector Laboratories, Burlingame, CA, USA) for MCH staining or in ImmPACT Vector BCIP/NBT Substrate chromogen solution (#SK-5400, Vector Laboratories, Burlingame, CA, USA) for HCRT staining with 1% 125 mM Levamisole (#31742, Sigma–Aldrich, Germany) to block endogenous alkaline phosphatase activity. .. After washing for 3 × 10 min in TBS, the sections were mounted on 0.5% gelatin (#24360.233, VWR chemicals, UK)/0.05% chrome alum (#A11816, Alfa Aesar, Germany)-coated superfrost glass slides (Hounisen, Aarhus, Denmark) and dried before subjected to a graded dehydration series using ethanol (70%, 96%, 99%) for 5 min per step, cleared in xylene for 10 min, and coverslipped with DPX new (#1.00579, Merck, Germany).



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    Image Search Results


    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Journal: Theranostics

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    doi: 10.7150/thno.11711

    Figure Lengend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Article Snippet: The chromogenic alkaline phosphatase solution (Vector Labs, Cat #SK-5100) was prepared according to the manufacturer's instruction and added to the slides following 1 h staining of secondary anti-biotin antibody (Vector Labs, Cat #SP-3020) (1: 250 dilution).

    Techniques: Double Staining, Staining, Injection