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vector red chromogen substrate  (Vector Laboratories)


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    Vector Laboratories vector red chromogen substrate
    Vector Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+chromogenic+substrate+kit/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc12628778-299-51-55
    Average 96 stars, based on 1935 article reviews
    vector red chromogen substrate - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy
    Article Snippet: .. The following day, sections were incubated in a biotinylated secondary antibody for 1 h followed by alkaline phosphatase-conjugated avidin (Vectastain ABC-AP Universal Kit; Vector Laboratories, Burlingame, CA) for 30 min and then visualized with the Vector Red chromogenic substrate kit (SK-5100; Vector Laboratories). .. For the Ki67 samples, tyramide signal amplification (TSA, PerkinElmer, Waltham, MA) was performed following incubation in biotinylated secondary antibody, and the Vector Red chromogenic substrate was added.

    Avidin-Biotin Assay:

    Article Title: Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy
    Article Snippet: .. The following day, sections were incubated in a biotinylated secondary antibody for 1 h followed by alkaline phosphatase-conjugated avidin (Vectastain ABC-AP Universal Kit; Vector Laboratories, Burlingame, CA) for 30 min and then visualized with the Vector Red chromogenic substrate kit (SK-5100; Vector Laboratories). .. For the Ki67 samples, tyramide signal amplification (TSA, PerkinElmer, Waltham, MA) was performed following incubation in biotinylated secondary antibody, and the Vector Red chromogenic substrate was added.

    Plasmid Preparation:

    Article Title: Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy
    Article Snippet: .. The following day, sections were incubated in a biotinylated secondary antibody for 1 h followed by alkaline phosphatase-conjugated avidin (Vectastain ABC-AP Universal Kit; Vector Laboratories, Burlingame, CA) for 30 min and then visualized with the Vector Red chromogenic substrate kit (SK-5100; Vector Laboratories). .. For the Ki67 samples, tyramide signal amplification (TSA, PerkinElmer, Waltham, MA) was performed following incubation in biotinylated secondary antibody, and the Vector Red chromogenic substrate was added.



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    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Image Search Results


    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Journal: Theranostics

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    doi: 10.7150/thno.11711

    Figure Lengend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Article Snippet: The chromogenic alkaline phosphatase solution (Vector Labs, Cat #SK-5100) was prepared according to the manufacturer's instruction and added to the slides following 1 h staining of secondary anti-biotin antibody (Vector Labs, Cat #SP-3020) (1: 250 dilution).

    Techniques: Double Staining, Staining, Injection