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vector red chromogen substrate  (Vector Laboratories)


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    Vector Laboratories vector red chromogen substrate
    Vector Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+chromogen+substrate/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc12628778-299-51-55
    Average 96 stars, based on 1934 article reviews
    vector red chromogen substrate - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1X PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1× PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Sequencing:

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1X PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1× PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Avidin-Biotin Assay:

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1X PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1× PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Article Title: Directional preference of airway smooth muscle mass increase in human asthmatic airways.
    Article Snippet: .. Sections were 117 then blocked in universal blocking solution (DakoCytomation, Glastrop, Denmark), and 118 antibodies to α-SMA (1A4, Sigma-Aldrich, St-Louis, MO) and proliferating cell nuclear antigen 119 (PCNA; CalBiochem) were detected with biotinylated rabbit anti-mouse IgG, avidin/biotin-120 alkaline phosphatase complex and VECTOR RED chromogen substrate (Vector Laboratories, 121 Burlington, ON). .. Apoptosis was detected by TUNEL with an ApopTag peroxidase kit (EMD 122 Millipore, Etobicoke, ON) and enhanced Diaminobenzidine (DAB)-Nickel (Vector Laboratories, 123 Burlington, ON), following manufacturer’s instructions.

    Staining:

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1X PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids
    Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Vector Red chromogen substrate (Vector Laboratories), according to manufacturer’s instruction. .. Between each incubation step, sections were rinsed with PBST (1× PBS, 0.1% Tween20) for 5 min. Tissues were counterstained with Meyer’s hematoxylin (Sigma-Aldrich), followed by 0.1% sodium bicarbonate (Sigma-Aldrich), and covered with CC/Mount (Sigma-Aldrich).

    Blocking Assay:

    Article Title: Directional preference of airway smooth muscle mass increase in human asthmatic airways.
    Article Snippet: .. Sections were 117 then blocked in universal blocking solution (DakoCytomation, Glastrop, Denmark), and 118 antibodies to α-SMA (1A4, Sigma-Aldrich, St-Louis, MO) and proliferating cell nuclear antigen 119 (PCNA; CalBiochem) were detected with biotinylated rabbit anti-mouse IgG, avidin/biotin-120 alkaline phosphatase complex and VECTOR RED chromogen substrate (Vector Laboratories, 121 Burlington, ON). .. Apoptosis was detected by TUNEL with an ApopTag peroxidase kit (EMD 122 Millipore, Etobicoke, ON) and enhanced Diaminobenzidine (DAB)-Nickel (Vector Laboratories, 123 Burlington, ON), following manufacturer’s instructions.



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    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Image Search Results


    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Journal: Theranostics

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    doi: 10.7150/thno.11711

    Figure Lengend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Article Snippet: The chromogenic alkaline phosphatase solution (Vector Labs, Cat #SK-5100) was prepared according to the manufacturer's instruction and added to the slides following 1 h staining of secondary anti-biotin antibody (Vector Labs, Cat #SP-3020) (1: 250 dilution).

    Techniques: Double Staining, Staining, Injection