vector red chromogen substrate (Vector Laboratories)
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Vector Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+red+chromogen+substrate/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc12628778-299-51-55
Average 96 stars, based on 1934 article reviews
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Incubation:Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Sequencing:Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Avidin-Biotin Assay:Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Article Title: Directional preference of airway smooth muscle mass increase in human asthmatic airways. Article Snippet: .. Sections were 117 then blocked in universal blocking solution (DakoCytomation, Glastrop, Denmark), and 118 antibodies to α-SMA (1A4, Sigma-Aldrich, St-Louis, MO) and proliferating cell nuclear antigen 119 (PCNA; CalBiochem) were detected with biotinylated rabbit anti-mouse IgG, avidin/biotin-120 alkaline phosphatase complex and Staining:Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1X PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 hour, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Article Title: Heritable viral symbionts in the family Iflaviridae are widespread among aphids Article Snippet: Sections were put through a standard deparaffinization and rehydration series and incubated in 1× PBS for 10 min. Antigen retrieval was achieved by submerging sections in citrate-EDTA buffer (10 mM citric acid, 2 mM EDTA, 0.05% Tween20, at pH 6.2) at 88°C for 20 min and cooling down the sections at room temperature before blocking with 6% casein (Sigma-Aldrich, St. Louis, MO, USA). .. We then incubated the sections with a 1:200 dilution of a polyclonal rabbit anti-BrBV capsid peptide antibody (sequence RGDLEFKFVLNSNKFC) (GenScript, Piscataway, NJ, USA) at room temperature for 1 h, followed by sequential incubation with biotinylated goat anti-rabbit, avidin-biotin alkaline phosphatase (VECTASTAIN ABC-AP Staining kit, Vector Laboratories, Inc., Newark, CA, USA), and Blocking Assay:Article Title: Directional preference of airway smooth muscle mass increase in human asthmatic airways. Article Snippet: .. Sections were 117 then blocked in universal blocking solution (DakoCytomation, Glastrop, Denmark), and 118 antibodies to α-SMA (1A4, Sigma-Aldrich, St-Louis, MO) and proliferating cell nuclear antigen 119 (PCNA; CalBiochem) were detected with biotinylated rabbit anti-mouse IgG, avidin/biotin-120 alkaline phosphatase complex and |
