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mammalian expression vectors prk5-megfp-hmgb1-wt  (Addgene inc)


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    Structured Review

    Addgene inc mammalian expression vectors prk5-megfp-hmgb1-wt
    Mammalian Expression Vectors Prk5 Megfp Hmgb1 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+prk5/prk5+ha+gst+raga+q66l/pm40468084-568-0-13
    Average 90 stars, based on 1 article reviews
    mammalian expression vectors prk5-megfp-hmgb1-wt - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Western Blot:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Ubiquitin Proteomics:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Plasmid Preparation:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Transfection:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Expressing:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Negative Control:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Control:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Immunofluorescence:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.

    Staining:

    Article Title: CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
    Article Snippet: Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.Myc-pcDNA3.1-CKIP-1 was provided by Fuchu He (State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine) [30], myc-pcDNA3.1 vector was preserved in our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, pRK5-HA-Ub K63, pRK-Myc-Smurf1 and pRK-Myc-Smurf1 delHECT were purchased from Addgene (http://www.addgene.org/).. PRK-Myc vector was constructed by our laboratory.PRK-Myc vector was constructed by our laboratory.

    Article Title: Sphingosine kinase 1 mediates AGEs-induced fibronectin upregulation in diabetic nephropathy
    Article Snippet: Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.Vector pEGFP, human full-length wild-type pEGFP-RAGE and human cytoplasmic-deleted mutant-type pEGFP-RAGEΔcyto were constructed and supplied by Genechem.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene ( http://www.addgene.org/ ).. GMCs were plated in 35 mm culture plates 24 h prior to transfection.GMCs were plated in 35 mm culture plates 24 h prior to transfection.

    Article Title: AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.
    Article Snippet: Vector pcDNA3 was preserved by our laboratory.Vector pcDNA3 was preserved by our laboratory.. PcDNA3-HA-Ub, pcDNA3-HA-Ub K0, vector pRK5, pRK5-HA-Ub, pRK5-HA-Ub K48, and pRK5-HA-Ub K63 were purchased from Addgene (http:// www.addgene.org/).. Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.Vector pEGFP, human full-length wildtype pEGFP-RAGE, human cytoplasmic-deleted mutant-type pEGFP-RAGE cyto, and rat wild-type pEGFP-USP22 were constructed and supplied by Genechem.



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: KRAS G12D -driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC

    doi: 10.1016/j.xcrm.2025.101966

    Figure Lengend Snippet:

    Article Snippet: In addition, the cDNA sequences of E2F1 and RING1 gene were cloned into the pRK5-HA vector. pCMV3-His-Rb (#HG10137-NH) was purchased from Sino Biological Inc. (Beijing, China). pRK5-HA-Ubi, pRK5- Ring1 , pRK5- UBE2T , deletions-mutant p53 plasmids were constructed in our previous article.

    Techniques: Mutagenesis, Recombinant, Virus, CCK-8 Assay, Membrane, Concentration Assay, Modification, Transfection, Agarose Gel Electrophoresis, Plasmid Preparation, Extraction, Bicinchoninic Acid Protein Assay, Staining, RNA Extraction, H&E Stain, Viability Assay, Reporter Assay, Labeling, Western Blot, Sequencing, Control, Software