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plx304 vector  (Addgene inc)


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    Structured Review

    Addgene inc plx304 vector
    A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and <t>pLX304</t> (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.
    Plx304 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+plx304/pLX304+(Plasmid+%2325890)/bio_rxiv__64898__2026__01__23__701386-148-16-18
    Average 95 stars, based on 297 article reviews
    plx304 vector - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "A simple method for analyzing competitive growth of multiple cell types in xenograft tumors"

    Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors

    Journal: bioRxiv

    doi: 10.64898/2026.01.23.701386

    A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.
    Figure Legend Snippet: A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

    Techniques Used:

    Related Articles

    other:

    Article Title: Grainyhead-Like 3 Influences Migration and Invasion of Urothelial Carcinoma Cells
    Article Snippet: The empty vector (pLX304) was acquired from Addgene and a 1688-bp PCR product of GRHL3 cDNA was cloned by Nhel and Xbal (New England Biolabs, Ipswich, MA, USA) into the pLX304 backbone.

    Over Expression:

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia.
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..

    Plasmid Preparation:

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia.
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..

    Cloning:

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia.
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..

    Mutagenesis:

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia.
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..

    Article Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia
    Article Snippet: .. For the overexpression of spRNAP-IV, the vector pLX304 (Addgene plasmid 25890) was employed, and Gateway cloning was used to insert a truncated version of POLRMT (starting with the ATG corresponding to a N262 truncation mutant), using as a template I.M.A.G.E. clone 6572256 that contains the POLRMT cDNA. ..



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    Image Search Results


    A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

    Journal: bioRxiv

    Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors

    doi: 10.64898/2026.01.23.701386

    Figure Lengend Snippet: A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

    Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites.

    Techniques:

    A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

    Journal: bioRxiv

    Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors

    doi: 10.64898/2026.01.23.701386

    Figure Lengend Snippet: A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

    Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites.

    Techniques:

    qPCR analysis of six pools of HCT116 cells, each with one of the six pLenti-puro based Tag vectors (Tag in cells: 1-6, shades of green). Following DNA isolation and pre-amplification, each sample was analyzed by qPCR for all six Tags as well as with the internal primer pair. Data are plotted (grouped by Tag primer pair used for qPCR) as the delta Ct (internal minus Tag amplicon).

    Journal: bioRxiv

    Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors

    doi: 10.64898/2026.01.23.701386

    Figure Lengend Snippet: qPCR analysis of six pools of HCT116 cells, each with one of the six pLenti-puro based Tag vectors (Tag in cells: 1-6, shades of green). Following DNA isolation and pre-amplification, each sample was analyzed by qPCR for all six Tags as well as with the internal primer pair. Data are plotted (grouped by Tag primer pair used for qPCR) as the delta Ct (internal minus Tag amplicon).

    Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites.

    Techniques: DNA Extraction, Amplification