vector pgbkt7 bd (TaKaRa)
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vector pgbkt7 bd
Vector Pgbkt7 Bd, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4593 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pgbkt7/pGBKT7+DNA-BD+Vector/bio_rxiv__64898__2026__05__09__724007-261-17-25
Average 99 stars, based on 4593 article reviews
Vector Pgbkt7 Bd, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4593 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pgbkt7/pGBKT7+DNA-BD+Vector/bio_rxiv__64898__2026__05__09__724007-261-17-25
Average 99 stars, based on 4593 article reviews
vector pgbkt7 bd - by Bioz Stars,
2026-10
99/100 stars
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Two Hybrid Screening:Article Title: Methods and materials for identifying and treating BET inhibitor-resistant cancers Article Snippet: .. Yeast Two-Hybrid Screen Yeast two-hybrid screen was performed with the full-length SPOP cloned in-frame with the GAL4 DNA binding domain in Clone Assay:Article Title: Methods and materials for identifying and treating BET inhibitor-resistant cancers Article Snippet: .. Yeast Two-Hybrid Screen Yeast two-hybrid screen was performed with the full-length SPOP cloned in-frame with the GAL4 DNA binding domain in Article Title: A noncanonical TNL immune hub defines separable recognition and signaling modules for clubroot resistance Article Snippet: .. To assess domain-specific interactions, two partial constructs, i.e., Rcr1-TAN (1–548 aa, encompassing T IR a nd N B-ARC), and Rcr1-LRR (570–1190 aa, covering LRR), were synthesized (IDT) and cloned into Article Title: The BnSEP-BnTFL1s module regulates inflorescence architecture based on light duration in Brassica napus L Article Snippet: Signal visualization was performed using the reagents provided in the kit on a ChemiDoc XRS system (Bio-Rad Laboratories, Hercules, CA) [ ]. .. The CDSs of BnaA10/C09.TFL1 were amplified and cloned into the Y2H ‘bait’ Binding Assay:Article Title: Methods and materials for identifying and treating BET inhibitor-resistant cancers Article Snippet: .. Yeast Two-Hybrid Screen Yeast two-hybrid screen was performed with the full-length SPOP cloned in-frame with the GAL4 DNA binding domain in Article Title: The Tartary buckwheat WRKY transcription factor FtWRKY28 positively regulates low phosphate stress tolerance in transgenic Arabidopsis plants. Article Snippet: Accumulating data suggest that WRKY transcription factor (TF) family is involved in the regulation of mineral nutrition stress in general and low-phosphate (low-Pi) in plants.. Buckwheat exhibits high efficiency of Pi uptake and wide adaptability to grow in under-fertilized soils.. However, the regulatory roles and underlying molecular mechanisms of WRKY TFs in buckwheat’s adaptation to low-Pi environments remain poorly understood. Amplification:Article Title: A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum. Article Snippet: Total RNA extraction fromP. oxalicumwasconductedusing anRNAsimpleTotalRNA Kit (Tiangen Biotech Co., Ltd., Beijing, China) according to the manufacturer’s instructions. .. DNA fragments were amplified by PCR with specific primers (Supplementary Table S2) using Δku70 cDNA as template, then subcloned into Article Title: A Novel Ah-miR2916-AhERF13-AhSUC3 Module Regulates Al Tolerance via Ethylene-Mediated Signaling in Peanut (Arachis hypogea L.). Article Snippet: Aluminum (Al) toxicity in acidic soils leads to a considerable reduction in crop yields.. MicroRNAs play essential roles in abiotic stress responses, but little is known of their role in the response of peanut (Arachis hypogea L.) to Al stress.. In this study, a novel Ah‐miR2916 (miR2916)–AhERF13–AhSUC3 module was found to be involved in the Al‐stress response via ethylene‐mediated signaling in peanut. Article Title: The Tartary buckwheat WRKY transcription factor FtWRKY28 positively regulates low phosphate stress tolerance in transgenic Arabidopsis plants. Article Snippet: Accumulating data suggest that WRKY transcription factor (TF) family is involved in the regulation of mineral nutrition stress in general and low-phosphate (low-Pi) in plants.. Buckwheat exhibits high efficiency of Pi uptake and wide adaptability to grow in under-fertilized soils.. However, the regulatory roles and underlying molecular mechanisms of WRKY TFs in buckwheat’s adaptation to low-Pi environments remain poorly understood. Article Title: The BnSEP-BnTFL1s module regulates inflorescence architecture based on light duration in Brassica napus L Article Snippet: Signal visualization was performed using the reagents provided in the kit on a ChemiDoc XRS system (Bio-Rad Laboratories, Hercules, CA) [ ]. .. The CDSs of BnaA10/C09.TFL1 were amplified and cloned into the Y2H ‘bait’ Polymerase Chain Reaction:Article Title: A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum. Article Snippet: Total RNA extraction fromP. oxalicumwasconductedusing anRNAsimpleTotalRNA Kit (Tiangen Biotech Co., Ltd., Beijing, China) according to the manufacturer’s instructions. .. DNA fragments were amplified by PCR with specific primers (Supplementary Table S2) using Δku70 cDNA as template, then subcloned into Plasmid Preparation:Article Title: A Novel Ah-miR2916-AhERF13-AhSUC3 Module Regulates Al Tolerance via Ethylene-Mediated Signaling in Peanut (Arachis hypogea L.). Article Snippet: Aluminum (Al) toxicity in acidic soils leads to a considerable reduction in crop yields.. MicroRNAs play essential roles in abiotic stress responses, but little is known of their role in the response of peanut (Arachis hypogea L.) to Al stress.. In this study, a novel Ah‐miR2916 (miR2916)–AhERF13–AhSUC3 module was found to be involved in the Al‐stress response via ethylene‐mediated signaling in peanut. Construct:Article Title: The Tartary buckwheat WRKY transcription factor FtWRKY28 positively regulates low phosphate stress tolerance in transgenic Arabidopsis plants. Article Snippet: Accumulating data suggest that WRKY transcription factor (TF) family is involved in the regulation of mineral nutrition stress in general and low-phosphate (low-Pi) in plants.. Buckwheat exhibits high efficiency of Pi uptake and wide adaptability to grow in under-fertilized soils.. However, the regulatory roles and underlying molecular mechanisms of WRKY TFs in buckwheat’s adaptation to low-Pi environments remain poorly understood. Article Title: A noncanonical TNL immune hub defines separable recognition and signaling modules for clubroot resistance Article Snippet: .. To assess domain-specific interactions, two partial constructs, i.e., Rcr1-TAN (1–548 aa, encompassing T IR a nd N B-ARC), and Rcr1-LRR (570–1190 aa, covering LRR), were synthesized (IDT) and cloned into Synthesized:Article Title: A noncanonical TNL immune hub defines separable recognition and signaling modules for clubroot resistance Article Snippet: .. To assess domain-specific interactions, two partial constructs, i.e., Rcr1-TAN (1–548 aa, encompassing T IR a nd N B-ARC), and Rcr1-LRR (570–1190 aa, covering LRR), were synthesized (IDT) and cloned into |
![Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a <t>GAL4‐activating</t> domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8646/pmc12948646/pmc12948646__MPP-27-e70202-g007.jpg)