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hard set mounting medium with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories hard set mounting medium with dapi
    Hard Set Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 3088 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectashield+hard+set/VECTASHIELD+Hardset+Antifade+Mounting+Medium+with+DAPI/pm42034131-140-5-11
    Average 96 stars, based on 3088 article reviews
    hard set mounting medium with dapi - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: TOB1 modulates neutrophil phenotypes to influence gastric cancer progression and immunotherapy efficacy.
    Article Snippet: Finally, the slides were counterstained with DAPI for 5 min and mounted using the VECTASHIELD Hard Set (H-1400; Vector Labs, Burlingame, CA, USA).

    Article Title: TOB1 modulates neutrophil phenotypes to influence gastric cancer progression and immunotherapy efficacy
    Article Snippet: Finally, the slides were counterstained with DAPI for 5 min and mounted using the VECTASHIELD Hard Set (H-1400; Vector Labs, Burlingame, CA, USA).

    Article Title: Intracerebroventricular phospho-tau immunotherapy alleviates cortical phospho-tau burden and motor phenotype in a neuron-specific P301S tauopathy mouse.
    Article Snippet: Pathological tau accumulation disrupts neuronal function, leading to neurodegeneration and dementia in tauopathies, such as Alzheimer’s disease and frontotemporal dementia.. Despite the progression of several antitau therapies to clinical trials, no disease-modifying treatments for tauopathies exist.. Tau hyperphosphorylation is a key factor in pathology progression.

    Microscopy:

    Article Title: A single dose of AC102 restores hearing in a guinea pig model of noise-induced hearing loss to almost prenoise levels
    Article Snippet: Secondary antibodies used were goat anti-mouse (IgG1)-AF568 at 1:500 (#A21124, Invitrogen, Waltham MA, USA), goat anti-mouse (IgG2a)-AF488 at 1:500 (#A21131, Invitrogen, Waltham MA, USA) and phalloidin 405 at 1:200 (phalloidin 405, #A30104, Thermo Fisher Scientific, Waltham, MA, USA). .. Afterwards, tissues were washed twice with PBST for 10 min, the sensory epithelium dissected out from the cochlear tissue, cut into sections and flat mounted in a tonotopic manner on microscope slides using VECTASHIELD® Hard Set (Cat. #H-1400, Vector laboratories, Newark, CA, USA) as a mounting agent. .. Image acquisition and histological assessment Leica SP5 confocal microscope (Wetzlar, Germany) was used for the imaging of the stained cochlear sections.

    Article Title: Mechanisms that clear mutations drive field cancerization in mammary tissue
    Article Snippet: Secondary antibodies diluted in blocking buffer were incubated for at least 6 h. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (0.1 μg ml −1 ; Sigma-Aldrich) in PBS. .. Glands were washed with PBS and mounted on a microscopy slide with Vectashield hard set (H-1400, Vector Laboratories). .. Primary antibodies used were: anti-KRT14 (rabbit, Covance, PRB155P, 1:700), anti-ECAD (rat, eBioscience, 14-3249-82, 1:700), anti-oestrogen receptor (rabbit, no. 13258, Cell Signaling, 1:100), anti-progesterone receptor (rabbit, Clone SP2, MA5-14505, ThermoFisher Scientific, 1:200) and anti-SMA (mouse IgG2a, clone 1A4, Sigma-Aldrich, 1:600).

    Article Title: Mechanisms that clear mutations drive field cancerization in mammary tissue.
    Article Snippet: Secondary antibodies diluted in blocking buffer were incubated for at least 6 h. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (0.1 μg ml−1; Sigma-Aldrich) in PBS. .. Glands were washed with PBS and mounted on a microscopy slide with Vectashield hard set (H-1400, Vector Laboratories). .. Primary antibodies used were: anti-KRT14 (rabbit, Covance, PRB155P, 1:700), anti-ECAD (rat, eBioscience, 14-3249-82, 1:700), anti-oestrogen receptor (rabbit, no. 13258, Cell Signaling, 1:100), anti-progesterone receptor (rabbit, Clone SP2, MA5-14505, ThermoFisher Scientific, 1:200) and anti-SMA (mouse IgG2a, clone 1A4, Sigma-Aldrich, 1:600).

    Incubation:

    Article Title: Role of Extracellular Vesicles in TSC Renal Cystogenesis.
    Article Snippet: .. Sections were blocked in PBS containing normal horse serum, 0.2% powdered skim milk, and 0.3% Triton X-100 for at least 60 min at room temperature before incubation with anti-CD63 and anti-ATP6V1B1/B2 (Santa Cruz; Dallas, TX, USA) at 4 ◦C for 24 h. Slides were washed in PBS for 3 × 10 min and incubated in Alexafluor secondary antibodies (Invitrogen; Eugene, OR, USA) at room temperature for 2 h. After drying, a coverslip was applied using VectaShield Hard-Set (Vector Labs; Newark, CA, USA) as the mounting medium. .. Images were obtained with a Zeiss LSM800 microscope utilizing Zen software (Version 3.4.91.000, Carl Zeiss, Oberkochen, Germany).

    Article Title: Role of Extracellular Vesicles in TSC Renal Cystogenesis
    Article Snippet: .. Sections were blocked in PBS containing normal horse serum, 0.2% powdered skim milk, and 0.3% Triton X-100 for at least 60 min at room temperature before incubation with anti-CD63 and anti-ATP6V1B1/B2 (Santa Cruz; Dallas, TX, USA) at 4 °C for 24 h. Slides were washed in PBS for 3 × 10 min and incubated in Alexafluor secondary antibodies (Invitrogen; Eugene, OR, USA) at room temperature for 2 h. After drying, a coverslip was applied using VectaShield Hard-Set (Vector Labs; Newark, CA, USA) as the mounting medium. .. Images were obtained with a Zeiss LSM800 microscope utilizing Zen software (Version 3.4.91.000, Carl Zeiss, Oberkochen, Germany).



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