vectashield mounting medium (Vector Laboratories)
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Vectashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectashield+antifade+with+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm42898335-91-74-77
Average 98 stars, based on 22145 article reviews
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Incubation:Article Title: Identification of potential new T cell activation molecules: a Bioinformatic Approach. Article Snippet: Then the cells were washed 3 times with PBS and incubated for 1 h with primary antibody: α-CD69 (1:20, Abcam, Ab181602), α-CD40 (1:20, Abcam, Ab13545), α-SYT10 (1:20, Novus Biologicals, NBP2-85861), α-RND3 (1:50, Abnova, H00000390-MOI), α-DORA (1:20, EuroScientific, DDX0220P-100), α-GAPDH (1:50, Abcam, Ab233396), αCD53 (1:20, Novus biologicals, NB500393), α-PIN1 (1:20, R&D Systems, MAB2294), Scientific Reports | (2024) 14:22219 9| https://doi.org/10.1038/s41598-024-73003-9 α-MDM4 (1:20, Novus Biologicals, NB100556) dissolved in 1X PBS with 2% FBS. .. After that, 3 washes were performed and then incubated for 1 h with the secondary antibodies α-mouse (1:300, Jackson Immuno Research, 115-454062) and α-rabbit (1:300, Jackson Immuno Research, 111-545-045) labeled with Alexa Fluor® 488, at the end 3 washes with PBS and one more with distilled water, then 15 uL mounting medium Article Title: Generation of iPSC line NIMHi033-A from an Indian patient with Autism Spectrum Disorder carrying mutation in DYNC1H1 gene Article Snippet: .. The following day, cells were washed thrice with 0.1% PBST, incubated with secondary antibodies for one hour, followed by 3 washes with 0.1% PBST, and then mounted with Labeling:Article Title: Identification of potential new T cell activation molecules: a Bioinformatic Approach. Article Snippet: Then the cells were washed 3 times with PBS and incubated for 1 h with primary antibody: α-CD69 (1:20, Abcam, Ab181602), α-CD40 (1:20, Abcam, Ab13545), α-SYT10 (1:20, Novus Biologicals, NBP2-85861), α-RND3 (1:50, Abnova, H00000390-MOI), α-DORA (1:20, EuroScientific, DDX0220P-100), α-GAPDH (1:50, Abcam, Ab233396), αCD53 (1:20, Novus biologicals, NB500393), α-PIN1 (1:20, R&D Systems, MAB2294), Scientific Reports | (2024) 14:22219 9| https://doi.org/10.1038/s41598-024-73003-9 α-MDM4 (1:20, Novus Biologicals, NB100556) dissolved in 1X PBS with 2% FBS. .. After that, 3 washes were performed and then incubated for 1 h with the secondary antibodies α-mouse (1:300, Jackson Immuno Research, 115-454062) and α-rabbit (1:300, Jackson Immuno Research, 111-545-045) labeled with Alexa Fluor® 488, at the end 3 washes with PBS and one more with distilled water, then 15 uL mounting medium Microscopy:Article Title: Identification of potential new T cell activation molecules: a Bioinformatic Approach. Article Snippet: Then the cells were washed 3 times with PBS and incubated for 1 h with primary antibody: α-CD69 (1:20, Abcam, Ab181602), α-CD40 (1:20, Abcam, Ab13545), α-SYT10 (1:20, Novus Biologicals, NBP2-85861), α-RND3 (1:50, Abnova, H00000390-MOI), α-DORA (1:20, EuroScientific, DDX0220P-100), α-GAPDH (1:50, Abcam, Ab233396), αCD53 (1:20, Novus biologicals, NB500393), α-PIN1 (1:20, R&D Systems, MAB2294), Scientific Reports | (2024) 14:22219 9| https://doi.org/10.1038/s41598-024-73003-9 α-MDM4 (1:20, Novus Biologicals, NB100556) dissolved in 1X PBS with 2% FBS. .. After that, 3 washes were performed and then incubated for 1 h with the secondary antibodies α-mouse (1:300, Jackson Immuno Research, 115-454062) and α-rabbit (1:300, Jackson Immuno Research, 111-545-045) labeled with Alexa Fluor® 488, at the end 3 washes with PBS and one more with distilled water, then 15 uL mounting medium Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues. Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goatantirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goat-antirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with Software:Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues. Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goatantirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goat-antirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with other:Article Title: Quercetin activates the SIRT6–Nrf2 axis during oxidative stress, modulating DNA repair and ageing-associated markers Article Snippet: Slides were blocked with 2.5 % |
