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Spring Bioscience antibody clone ve1
Antibody Clone Ve1, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ve1+antibody/anti+braf+v600e/pm40815143-8-6-7
Average 86 stars, based on 1 article reviews
antibody clone ve1 - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: BRAF V600E Mutation Lacks Association with Poorer Clinical Prognosis in Papillary Thyroid Carcinoma.
Article Snippet: Background.. Previous literatures showed wide range of prevalence of BRAF V600E in papillary thyroid carcinoma (PTC).. The correlation of BRAF V600E mutation with aggressive tumor characteristics and poor prognosis is controversial.

Sequencing:

Article Title: BRAF V600E Mutation Lacks Association with Poorer Clinical Prognosis in Papillary Thyroid Carcinoma.
Article Snippet: Background.. Previous literatures showed wide range of prevalence of BRAF V600E in papillary thyroid carcinoma (PTC).. The correlation of BRAF V600E mutation with aggressive tumor characteristics and poor prognosis is controversial.

Article Title: A Shift in Molecular Drivers of Papillary Thyroid Carcinoma Following the 2017 World Health Organization Classification: Characterization of 554 Consecutive Tumors With Emphasis on BRAF-Negative Cases.
Article Snippet: Most studies for comprehensive molecular profiling of papillary thyroid carcinoma (PTC) have been performed before the 2017 World Health Organization (WHO) classification, in which the diagnostic criteria of follicular variants of PTC have been modified and noninvasive follicular thyroid neoplasm with papillary-like nuclear features has been introduced.. This study aims to investigate the shift in the incidence of BRAF V600E mutations in PTCs following the 2017 WHO classification and to further characterize the histologic subtypes and molecular drivers in BRAF-negative cases.. The study cohort consisted of 554 consecutive PTCs larger than 0.5 cm between January 2019 and May 2022.

Immunohistochemical staining:

Article Title: BRAF V600E Mutation Lacks Association with Poorer Clinical Prognosis in Papillary Thyroid Carcinoma.
Article Snippet: Background.. Previous literatures showed wide range of prevalence of BRAF V600E in papillary thyroid carcinoma (PTC).. The correlation of BRAF V600E mutation with aggressive tumor characteristics and poor prognosis is controversial.

Article Title: Distinct clinicopathological features in metanephric adenoma harboring BRAF mutation
Article Snippet: .. Immunohistochemical analysis was performed on all cases utilizing the VE1 antibody, which recognizes the BRAF V600E mutant protein (Spring Bioscience, Pleasanton, CA, USA), on whole tissue sections. ..

other:

Article Title: BRAFV600E and MAP2K1 mutations in Langerhans cell histiocytosis occur predominantly in children.
Article Snippet: Department of Pathology, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka, Japan Correspondence Ying Guo, Department of Pathology, Xijing Hospital, Preclinical School, Fourth Military Medical University, Changlexi Street No.169, Xi'an City, Shaanxi Province, China.. Email: guoying@fmmu.edu.cn

Article Title: Giant cell glioblastoma is a distinctive subtype of glioma characterized by vulnerability to DNA damage
Article Snippet: Immunohistochemistry (IHC) using a Leica BOND-III (Leica, Newcastle Upon Tyne, UK) after antigen retrieval on 4-μm-thick FFPE sections was conducted using antibodies against IDH1 (H09, 1:200; Dianova, Hamburg, Germany), ATRX (polyclonal, 1:1000; ATRAS Antibodies, Stockholm, Sweden), BRAF V600E (VE1, 1:50; Spring Bioscience, Pleasanton, CA), GFAP (6F2, 1:400; DAKO, Carpinteria, CA), OLIG2 (polyclonal, 1:300; IBL, Takasaki, Japan), p53 (DO-7, prediluted; Leica), Ki67 (MIB1, 1:200; DAKO), γH2AX (JBW301, 1:400; Merck Millipore, Temecula, CA), CD133 (W6B3C1, 1:10; Miltenyi Biotec, Bergisch Gladbach, Germany), CD44 (DF 1485, 1:30; Abnova, Taipei, Taiwan), Nestin (10C2, 1:200; Merck Millipore), and podocalyxin-like protein (PODXL) (EPR9518, 1:1000; Abcam plc, Cambridge, UK).

Mutagenesis:

Article Title: Distinct clinicopathological features in metanephric adenoma harboring BRAF mutation
Article Snippet: .. Immunohistochemical analysis was performed on all cases utilizing the VE1 antibody, which recognizes the BRAF V600E mutant protein (Spring Bioscience, Pleasanton, CA, USA), on whole tissue sections. ..

Article Title: Comprehensive population-wide analysis of Lynch syndrome in Iceland reveals founder mutations in MSH6 and PMS2
Article Snippet: Polymerase chain reaction is used to amplify the region and the degree of methylation of four CpG sites is analysed in a single pyrosequencing reaction by taking the average of four sites. .. In cases where only biopsy tissue was available and MLH1 -hm testing failed, BRAF immunohistochemistry for the V600E mutation was performed as previously described using the VE1 antibody (1:700, incubation 15 min; Spring Bioscience, Pleasanton, CA) with an automated staining system (Bond Autostainer; Leica Microsystems, Buffalo Grove, IL) . ..

Immunohistochemistry:

Article Title: A Shift in Molecular Drivers of Papillary Thyroid Carcinoma Following the 2017 World Health Organization Classification: Characterization of 554 Consecutive Tumors With Emphasis on BRAF-Negative Cases.
Article Snippet: Most studies for comprehensive molecular profiling of papillary thyroid carcinoma (PTC) have been performed before the 2017 World Health Organization (WHO) classification, in which the diagnostic criteria of follicular variants of PTC have been modified and noninvasive follicular thyroid neoplasm with papillary-like nuclear features has been introduced.. This study aims to investigate the shift in the incidence of BRAF V600E mutations in PTCs following the 2017 WHO classification and to further characterize the histologic subtypes and molecular drivers in BRAF-negative cases.. The study cohort consisted of 554 consecutive PTCs larger than 0.5 cm between January 2019 and May 2022.

Article Title: Immunohistochemical detection of the BRAF V600E mutant protein in colorectal cancers in Taiwan is highly concordant with the molecular test.
Article Snippet: Aims: The aims of this study were to investigate the incidence of BRAF mutations in colorectal cancers (CRCs) in Taiwan and the sensitivity and specificity of VE1 immunohistochemistry in detecting the BRAF mutation.. Methods and results: A total of 425 resected colorectal adenocarcinoma specimens were recruited into this study.. Direct Sanger sequencing of exon 15 of the BRAF gene was performed for all cases.

Article Title: Metastatic thyroid carcinoma without identifiable primary tumor within the thyroid gland: a retrospective study of a rare phenomenon
Article Snippet: .. BRAF immunohistochemistry Six cases (Cases #1, 2, 3, 4, 6, and 7) with available material were subjected to immunohistochemistry analysis using the VE1 antibody directed against the BRAF V600E mutated protein (Spring Bioscience, Pleasanton, California, 1:50 dilution) in the nodal metastasis. .. The procedure was performed using the Ventana semiautomated staining system (Ventana Medical Systems, Inc., Tucson, Arizona).

Article Title: Comprehensive population-wide analysis of Lynch syndrome in Iceland reveals founder mutations in MSH6 and PMS2
Article Snippet: Polymerase chain reaction is used to amplify the region and the degree of methylation of four CpG sites is analysed in a single pyrosequencing reaction by taking the average of four sites. .. In cases where only biopsy tissue was available and MLH1 -hm testing failed, BRAF immunohistochemistry for the V600E mutation was performed as previously described using the VE1 antibody (1:700, incubation 15 min; Spring Bioscience, Pleasanton, CA) with an automated staining system (Bond Autostainer; Leica Microsystems, Buffalo Grove, IL) . ..

Staining:

Article Title: A Shift in Molecular Drivers of Papillary Thyroid Carcinoma Following the 2017 World Health Organization Classification: Characterization of 554 Consecutive Tumors With Emphasis on BRAF-Negative Cases.
Article Snippet: Most studies for comprehensive molecular profiling of papillary thyroid carcinoma (PTC) have been performed before the 2017 World Health Organization (WHO) classification, in which the diagnostic criteria of follicular variants of PTC have been modified and noninvasive follicular thyroid neoplasm with papillary-like nuclear features has been introduced.. This study aims to investigate the shift in the incidence of BRAF V600E mutations in PTCs following the 2017 WHO classification and to further characterize the histologic subtypes and molecular drivers in BRAF-negative cases.. The study cohort consisted of 554 consecutive PTCs larger than 0.5 cm between January 2019 and May 2022.

Article Title: Comprehensive population-wide analysis of Lynch syndrome in Iceland reveals founder mutations in MSH6 and PMS2
Article Snippet: Polymerase chain reaction is used to amplify the region and the degree of methylation of four CpG sites is analysed in a single pyrosequencing reaction by taking the average of four sites. .. In cases where only biopsy tissue was available and MLH1 -hm testing failed, BRAF immunohistochemistry for the V600E mutation was performed as previously described using the VE1 antibody (1:700, incubation 15 min; Spring Bioscience, Pleasanton, CA) with an automated staining system (Bond Autostainer; Leica Microsystems, Buffalo Grove, IL) . ..

Next-Generation Sequencing:

Article Title: A Shift in Molecular Drivers of Papillary Thyroid Carcinoma Following the 2017 World Health Organization Classification: Characterization of 554 Consecutive Tumors With Emphasis on BRAF-Negative Cases.
Article Snippet: Most studies for comprehensive molecular profiling of papillary thyroid carcinoma (PTC) have been performed before the 2017 World Health Organization (WHO) classification, in which the diagnostic criteria of follicular variants of PTC have been modified and noninvasive follicular thyroid neoplasm with papillary-like nuclear features has been introduced.. This study aims to investigate the shift in the incidence of BRAF V600E mutations in PTCs following the 2017 WHO classification and to further characterize the histologic subtypes and molecular drivers in BRAF-negative cases.. The study cohort consisted of 554 consecutive PTCs larger than 0.5 cm between January 2019 and May 2022.

Incubation:

Article Title: Immunohistochemical detection of the BRAF V600E mutant protein in colorectal cancers in Taiwan is highly concordant with the molecular test.
Article Snippet: Aims: The aims of this study were to investigate the incidence of BRAF mutations in colorectal cancers (CRCs) in Taiwan and the sensitivity and specificity of VE1 immunohistochemistry in detecting the BRAF mutation.. Methods and results: A total of 425 resected colorectal adenocarcinoma specimens were recruited into this study.. Direct Sanger sequencing of exon 15 of the BRAF gene was performed for all cases.

Article Title: Comprehensive population-wide analysis of Lynch syndrome in Iceland reveals founder mutations in MSH6 and PMS2
Article Snippet: Polymerase chain reaction is used to amplify the region and the degree of methylation of four CpG sites is analysed in a single pyrosequencing reaction by taking the average of four sites. .. In cases where only biopsy tissue was available and MLH1 -hm testing failed, BRAF immunohistochemistry for the V600E mutation was performed as previously described using the VE1 antibody (1:700, incubation 15 min; Spring Bioscience, Pleasanton, CA) with an automated staining system (Bond Autostainer; Leica Microsystems, Buffalo Grove, IL) . ..

Polymer:

Article Title: Immunohistochemical detection of the BRAF V600E mutant protein in colorectal cancers in Taiwan is highly concordant with the molecular test.
Article Snippet: Aims: The aims of this study were to investigate the incidence of BRAF mutations in colorectal cancers (CRCs) in Taiwan and the sensitivity and specificity of VE1 immunohistochemistry in detecting the BRAF mutation.. Methods and results: A total of 425 resected colorectal adenocarcinoma specimens were recruited into this study.. Direct Sanger sequencing of exon 15 of the BRAF gene was performed for all cases.



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(a) Chromatogram: BRAF <t>V600E</t> -mutated PTC. (b) PTC scored 8 for <t>VE1;</t> FA (inset) scored 6 (400×). (c) ROC curve: BRAF V600E IHC. (d) PTC: pMEK1/2 IHC score III (400×). (e) NIFTP: patchy pERK1/2 immunopositivity (400×). (f) DFS curves: BRAF V600E -mutated and BRAF V600E -wt cases
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Image Search Results


(a) Chromatogram: BRAF V600E -mutated PTC. (b) PTC scored 8 for VE1; FA (inset) scored 6 (400×). (c) ROC curve: BRAF V600E IHC. (d) PTC: pMEK1/2 IHC score III (400×). (e) NIFTP: patchy pERK1/2 immunopositivity (400×). (f) DFS curves: BRAF V600E -mutated and BRAF V600E -wt cases

Journal: Indian Journal of Endocrinology and Metabolism

Article Title: Elucidating the Prognostic Role of BRAF V600E and the Activation Status of the Downstream MAPK Pathway in PTC: A Study from a Tertiary Centre in India

doi: 10.4103/ijem.ijem_235_23

Figure Lengend Snippet: (a) Chromatogram: BRAF V600E -mutated PTC. (b) PTC scored 8 for VE1; FA (inset) scored 6 (400×). (c) ROC curve: BRAF V600E IHC. (d) PTC: pMEK1/2 IHC score III (400×). (e) NIFTP: patchy pERK1/2 immunopositivity (400×). (f) DFS curves: BRAF V600E -mutated and BRAF V600E -wt cases

Article Snippet: Five-micron-thick formalin-fixed paraffin-embedded representative sections were stained for BRAF V600E (clone VE1, 1:50; Spring Bioscience) by using the Ventana automated immunohistochemical stainer according to the manufacturer’s specifications (Ventana Medical Systems, Tucson, AZ).

Techniques: