vaginal lactobacilli l crispatus atcc  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

doi: 10.3390/ijms222413631

Figure Lengend Snippet: Concentration-dependent fluorescence of lactobacilli expressing different fluorescent proteins. Linear regression analyses of fluorescence and OD 600 of lactobacilli grown at 37 °C without and with shaking (aeration) are shown. Nontransformed (NT) bacteria were used as controls. FU—fluorescence unit.

Article Snippet: Here, we engineered three model vaginal lactobacilli ( Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2).

Techniques: Concentration Assay, Fluorescence, Expressing, Bacteria

Journal: International Journal of Molecular Sciences

Article Title: Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

doi: 10.3390/ijms222413631

Figure Lengend Snippet: Representative confocal microscopy images of the lactobacilli expressing the different fluorescent proteins, as L. plantarum expressing IRFP, L. gasseri expressing mCherry, L. crispatus expressing GFP, and L. jensenii expressing mTagBFP2, in comparison to the nontransformed bacteria.

Article Snippet: Here, we engineered three model vaginal lactobacilli ( Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2).

Techniques: Confocal Microscopy, Expressing, Comparison, Bacteria

Journal: International Journal of Molecular Sciences

Article Title: Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

doi: 10.3390/ijms222413631

Figure Lengend Snippet: Distinction between the same species of Lactobacillus expressing different fluorescent proteins. Fluorescence was measured for the individual fluorescent strains and their mixtures, using settings corresponding to all four of the fluorescent proteins. The ratios indicate the proportions of the species expressing the fluorescent proteins in the following order: IRFP:GFP:mCherry:mTagBFP2. The encircled graphs represent the combination of fluorescent proteins and lactobacilli that were selected for further studies. * p < 0.05 (Student’s t tests) relative to nontransformed strain (NT, high-lighted with # for clarity). FU—fluorescence units; PBS—phosphate-buffered saline.

Article Snippet: Here, we engineered three model vaginal lactobacilli ( Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2).

Techniques: Expressing, Fluorescence, Saline

Journal: International Journal of Molecular Sciences

Article Title: Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

doi: 10.3390/ijms222413631

Figure Lengend Snippet: Fluorescence-based distinction of the different fluorescent lactobacilli and the nontransformed (NT) lactobacilli. Fluorescence was measured for the individual fluorescent species or their mixtures using the settings for all four of the fluorescent proteins. The ratios indicate the proportions of species in the mixtures in the following order: L. plantarum expressing IRFP; L. crispatus expressing GFP; L. gasseri expressing mCherry; and L. jensenii expressing mTagBFP2. * p < 0.05 (Student’s t tests), obtained by comparing fluorescent strain (F) to its nontransformed counterpart (NT). Lpl— L. plantarum ; Lga— L. gasseri ; Lcr— L. crispatus ; Lje— L. jensenii ; PBS—phosphate-buffered saline.

Article Snippet: Here, we engineered three model vaginal lactobacilli ( Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2).

Techniques: Fluorescence, Expressing, Saline

Journal: International Journal of Molecular Sciences

Article Title: Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

doi: 10.3390/ijms222413631

Figure Lengend Snippet: Representative confocal microscopy images of the mixtures of the lactobacilli expressing the different fluorescent proteins (F). Lpl— L. plantarum ; Lga— L. gasseri ; Lcr— L. crispatus ; Lje— L. jensenii ; NT—nontransformed species. Fluorescence images were obtained by using settings for denoted fluorescent proteins and merging thus obtained images, whereby settings for all four fluorescent proteins were used for the mixture.

Article Snippet: Here, we engineered three model vaginal lactobacilli ( Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2).

Techniques: Confocal Microscopy, Expressing, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

doi: 10.3390/ijms222413631

Figure Lengend Snippet: Scanning electron microscopy images of individual lactobacilli and as mixtures under high and low magnification. Columns 1 and 3, air-dried bacteria from water suspensions; Columns 2 and 4, bacteria incorporated into nanofibers. Average nanofiber diameters are specified in the last column.

Article Snippet: Here, we engineered three model vaginal lactobacilli ( Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2).

Techniques: Electron Microscopy, Bacteria

Journal: International Journal of Molecular Sciences

Article Title: Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

doi: 10.3390/ijms222413631

Figure Lengend Snippet: Representative confocal microscopy images of the fluorescent lactobacilli and the nontransformed lactobacilli when incorporated in PEO electrospun nanofibers individually or as mixture of all 4 fluorescent lactobacilli.

Article Snippet: Here, we engineered three model vaginal lactobacilli ( Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2).

Techniques: Confocal Microscopy