usp11 knockdown  (MedChemExpress)

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: USP11 expression is upregulated in HCC.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques: Expressing

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: USP11 is associated with the proliferation and migration of HCC cells.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques: Migration

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: USP11 inhibits erastin-induced ferroptosis in HCC cells.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques:

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: USP11 is associated with the sensitivity of HCC cells to taxanes.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques:

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: USP11 stabilizes NRF2 through deubiquitination.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques:

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: USP11 inhibits erastin-induced ferroptosis in HCC cells through NRF2.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques:

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: USP11 inhibits the sensitivity of HCC cells to taxanes through NRF2.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques:

Journal: Translational Oncology

Article Title: USP11 is involved in the sensitivity of liver cancer cells to ferroptosis and taxanes through the regulation of NRF2 ubiquitin-mediated degradation

doi: 10.1016/j.tranon.2025.102553

Figure Lengend Snippet: In vivo knockdown of USP11 inhibits tumor growth and promotes ferroptosis.

Article Snippet: To trigger ferroptosis, cells with either USP11 overexpression or USP11 knockdown were exposed to erastin (20 μM; HY-15763; MedChemExpress, USA), a ferroptosis inducer, for 24 hours.

Techniques: In Vivo, Knockdown

Journal: Acta Neuropathologica Communications

Article Title: X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing’s disease in women

doi: 10.1186/s40478-025-01938-9

Figure Lengend Snippet: RNA sequencing identified USP11 as a susceptibility factor for women with functioning ACTH PitNETs. ( A ) Differential expression of deubiquitinating enzymes in male and female Cushing’s disease based on our data and the dataset from EMBL-EBI Array Express (accession numbers E-MTAB-7768). ( B ) The expression of USP11 in female and male PitNETs (accession numbers E-MTAB-7768). Female Cushing’s disease = 21, male Cushing’s disease = 5. Data are presented as mean ± SEM values. ( C ) The expression of USP11 in female and male PitNETs. Female Cushing’s disease = 13, male Cushing’s disease = 5. Data are presented as mean ± SEM values. ( D ) The AtT-20 cells were infected with indicated genes and then immunoblotted. ( E ) The representative IHC images of USP11 in normal pituitary, male and female functioning ACTH PitNETs with analysis. Scale bar, 50 μm. Normal pituitary = 5, female Cushing’s disease = 13, male Cushing’s disease = 5. Data are presented as mean ± SEM values. ** p < 0.01, *** p < 0.001

Article Snippet: Mouse USP11 knockdown adenoviruses were purchased from Vigene Biosciences.

Techniques: RNA Sequencing Assay, Expressing, Infection

Journal: Acta Neuropathologica Communications

Article Title: X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing’s disease in women

doi: 10.1186/s40478-025-01938-9

Figure Lengend Snippet: USP11 regulates POMC expression and ACTH secretion. ( A ) qPCR showed that USP11 promoted the transcription of Pomc in AtT-20 cells. Data are presented as mean ± SEM values. n = 3. *** p < 0.001. ( B-C ) Knockdown of USP11 suppressed the protein ( C ) and mRNA ( D ) expression of POMC in AtT-20 cells. Data are presented as mean ± SEM values. ** p < 0.01. ( D-E ) USP11 regulated the secretion of ACTH in AtT-20 cells. The secretion of ACTH from stable AtT-20 cells with USP11 overexpression ( D ) or knockdown ( E ) was measured by ELISA. Data are presented as mean ± SEM values. n = 5. ** p < 0.01, *** p < 0.001. ( F ) Immunohistochemistry of xenograft tumors. Representative IHC images of USP11, POMC and ACTH in xenograft tumors with USP11 knockdown compared to control. Scale bar, 50 μm. ( G ) Knockdown of USP11 inhibited ACTH secretion in vivo, which was measured by ELISA in nude mice with xenograft tumors. Data are presented as mean ± SEM values. n = 5. *** p < 0.001

Article Snippet: Mouse USP11 knockdown adenoviruses were purchased from Vigene Biosciences.

Techniques: Expressing, Knockdown, Over Expression, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Control, In Vivo

Journal: Acta Neuropathologica Communications

Article Title: X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing’s disease in women

doi: 10.1186/s40478-025-01938-9

Figure Lengend Snippet: USP11 interacts with the transcription factor TPIT. ( A ) Flowchart for identifying potential proteins that interact with USP11. USP11 and its interacting proteins were immunoprecipitated and identified using mass spectrometry. ( B-C ) Interaction of TPIT with USP11. External (B) and endogenous ( C ) Co-IP experiments were performed to detect the interaction between TPIT and USP11. ( D ) The direct interaction between USP11 and TPIT was determined by GST pulldown assay. ( E ) Co-localization of Flag-TPIT and USP11 in AtT20 cells. Scale bar, 5 μm

Article Snippet: Mouse USP11 knockdown adenoviruses were purchased from Vigene Biosciences.

Techniques: Immunoprecipitation, Mass Spectrometry, Co-Immunoprecipitation Assay, GST Pulldown Assay

Journal: Acta Neuropathologica Communications

Article Title: X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing’s disease in women

doi: 10.1186/s40478-025-01938-9

Figure Lengend Snippet: USP11 decreases the ubiquitination of TPIT. ( A ) USP11 suppressed the ubiquitination of TPIT. HEK293T cells were transfected with the indicated plasmids, and cell lysates were analyzed by ubiquitination assays. ( B ) Knockdown of USP11 increased TPIT ubiquitination. ( C ) Schematic showing wild-type USP11 and its truncated forms. ( D ) USP11 lacking the USP domain was unable to interact with TPIT. The interaction between Flag-TPIT and Myc-tagged USP11 or it truncated mutants was detected by Co-IP. ( E ) Ubiquitination assays showed that USP11 lacking the USP domains failed to deubiquitinate TPIT. ( F ) Ubiquitination assays showed reduced deubiquitination of TPIT with the 4KR, 5KR, and 12KR mutations by USP11. ( G ) Ubiquitination assays demonstrated that overexpression of USP11 resulted in a decrease in TPIT ubiquitination by TRIM65

Article Snippet: Mouse USP11 knockdown adenoviruses were purchased from Vigene Biosciences.

Techniques: Transfection, Knockdown, Co-Immunoprecipitation Assay, Over Expression

Journal: Acta Neuropathologica Communications

Article Title: X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing’s disease in women

doi: 10.1186/s40478-025-01938-9

Figure Lengend Snippet: USP11 mediates the proteasomal degradation of TPIT. ( A ) Overexpression of USP11 increased TPIT protein levels in Flag-TPIT stable HEK-293T and AtT-20 cells. ( B ) USP11 overexpression mitigated the reduction of TPIT levels induced by TRIM65 in Flag-TPIT stable HEK293T cells. ( C-D ) CHX chase assays showed that overexpression of USP11 extend the half-life of TPIT in Flag-TPIT stable HEK-293T ( C ) and AtT-20 ( D ) cells. The cells were treated with CHX (100 µg/mL) for 0, 3, or 6 h. The relative ratio of TPIT/actin was determined by ImageJ. Data are presented as mean ± SEM values. n.s., not significant; n = 3. *** p < 0.001. ( E-F ) CHX chase assays showed that knockdown of USP11 decrease the half-life of TPIT in Flag-TPIT stable HEK-293T ( E ) and AtT-20 ( F ) cells. The cells were treated with CHX (100 µg/mL) for 0, 3, or 6 h. The relative ratio of TPIT/actin was determined by ImageJ. Data are presented as mean ± SEM values. n.s., not significant; n = 3. *** p < 0.001. ( G ) Proteasome inhibitor MG132 (20 µM) reversed the decrease in TPIT protein levels caused by the knockdown of USP11 in AtT-20 cells. ( H ) Immunohistochemistry of xenograft tumors. Representative IHC images of TPIT in xenograft tumors with USP11 knockdown compared to control. Scale bar, 50 μm

Article Snippet: Mouse USP11 knockdown adenoviruses were purchased from Vigene Biosciences.

Techniques: Over Expression, Knockdown, Immunohistochemistry, Control

Journal: Acta Neuropathologica Communications

Article Title: X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing’s disease in women

doi: 10.1186/s40478-025-01938-9

Figure Lengend Snippet: Lomitapide and Nicergoline inhibit USP11 function, reducing POMC expression and ACTH secretion. ( A ) The binding pocket of the USP11 protein was predicted utilizing PLI, and the binding modes for Lomitapide and Nicergoline with the USP11 protein were subsequently analyzed. ( B ) Bemcentinib, AC628, Lomitapide, and Nicergoline reduced TPIT and POMC protein levels in AtT-20 cells. ( C ) The effects of Bemcentinib, AC628, Lomitapide, and Nicergoline on TPIT ubiquitination were assessed by ubiquitination assays in HEK-293T cells. ( D ) Lomitapide and Nicergoline did not affect TPIT ubiquitination following USP11 knockdown in HEK-293T cells. ( E ) Lomitapide and Nicergoline both suppressed Pomc in AtT-20 cells. Cells were treated with Lomitapide (1 µM) and Nicergoline (1 µM) and Pomc expression was measured by qPCR. Data are presented as mean ± SEM values. n = 3. *** p < 0.001. ( F ) As the concentrations of Lomitapide and Nicergoline increased from 0 to 1 µM, the protein levels of POMC and TPIT decreased. ( G ) Knockdown of USP11 prevented the decrease of TPIT and POMC protein by Lomitapide and Nicergoline. ( H ) Proteasome inhibitor MG132 (20 µM) reversed the decrease in TPIT protein levels caused by Lomitapide and Nicergoline in AtT-20 cells. ( I ) Lomitapide and Nicergoline inhibited ACTH secretion in AtT-20 cells, which was measured by ELISA. Data are presented as mean ± SEM values. n = 4. ** p < 0.01, *** p < 0.001. ( J ) The representative IHC images of TPIT, POMC and ACTH in xenograft tumors treated with Lomitapide and Nicergoline. Scale bar, 50 μm. ( K ) Lomitapide and Nicergoline inhibited the secretion of ACTH in vivo, which was measured by ELISA in nude mice with xenograft tumors. Data are presented as mean ± SEM values. n = 5. ** p < 0.01, *** p < 0.001

Article Snippet: Mouse USP11 knockdown adenoviruses were purchased from Vigene Biosciences.

Techniques: Expressing, Binding Assay, Knockdown, Enzyme-linked Immunosorbent Assay, In Vivo

Journal: Acta Neuropathologica Communications

Article Title: X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing’s disease in women

doi: 10.1186/s40478-025-01938-9

Figure Lengend Snippet: USP11-mediated TPIT deubiquitination promotes the susceptibility of functioning ACTH adenoma in women

Article Snippet: Mouse USP11 knockdown adenoviruses were purchased from Vigene Biosciences.

Techniques: