Review



usp10 cdna  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc usp10 cdna
    Usp10 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usp10+cdna/Flag-HA-USP10+(Plasmid+%2322543)/pmc05665446-595-0-9
    Average 93 stars, based on 17 article reviews
    usp10 cdna - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Over Expression:

    Article Title: The deubiquitylase USP10 regulates integrin β1 and β5 and fibrotic wound healing
    Article Snippet: For additional USP10 siRNA control experiments, HCFs were transfected with two USP10 siRNAs (Dharmacon) that target unique USP10 sequences from the pool of USP10 siRNAs (5′-UGAGUUUGGUGUCGAUGAA-3′ and 5′-GAUAAAAUCGUGAGGGAUA-3′). .. USP10 cDNA (Flag-HA-USP10; 22543) for transient overexpression was from Addgene [deposited by Wade Harper ( Sowa et al., 2009 )]. .. Lentiviral cDNA USP10 (pLenti-GIII-CMV-hUSP10-Cterm-HA) and Vector (pLenti-III-Blank Control Vector) were from ABM Applied Biological Material.

    Article Title: RNAi inhibition of USP10 to treat ocular disorders
    Article Snippet: USP10 siRNA was from Santa Cruz (sc-76811). .. USP10 cDNA (Flag-HA-USP10; 22543) for transient overexpression was from Addgene. .. Transient Transfections and Cell Lines Transient transfection was performed using the Amaxa Nucleofection system (Gaithersburg, Md.) and Lonza P3 reagent.



    Similar Products

    usp10 flag cdna  (Millipore)
    90
    Millipore usp10 flag cdna
    <t>USP10</t> overexpression promoted integrin recycling and fibronectin endocytosis. ( A – F ) Live cell integrin recycling assay. HCFs were transfected with 2ug control or USP10 cDNA. 48 hours post transfection, integrin recycling was detected with anti-rabbit-488 antibody prior to imaging by live cell confocal microscopy. Scale bar : 50 µm. ( C ) Fluorescence intensity of α5β1 integrin recycling fold change (1.9; P < 0.001) and ( F ) αv (1.7; P < 0.05) when USP10 was overexpressed. ( G ) FN ELISA. HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours prior to detachment and lysing. Endocytosed FN was quantified with the Quantikine ELISA kit. USP10 overexpression induced an increase internalized fibronectin fold change (2.1; P < 0.05, Student's t -test). Two primary cell lines (total N = 3 repeats). Five images per condition/per experiment were analyzed.
    Usp10 Flag Cdna, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usp10+cdna/anti+usp10/pmc08543399-51-10-13
    Average 90 stars, based on 1 article reviews
    usp10 flag cdna - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    usp10 (nm_005153) human tagged orf clone  (OriGene)
    90
    OriGene usp10 (nm_005153) human tagged orf clone
    <t>USP10</t> overexpression promoted integrin recycling and fibronectin endocytosis. ( A – F ) Live cell integrin recycling assay. HCFs were transfected with 2ug control or USP10 cDNA. 48 hours post transfection, integrin recycling was detected with anti-rabbit-488 antibody prior to imaging by live cell confocal microscopy. Scale bar : 50 µm. ( C ) Fluorescence intensity of α5β1 integrin recycling fold change (1.9; P < 0.001) and ( F ) αv (1.7; P < 0.05) when USP10 was overexpressed. ( G ) FN ELISA. HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours prior to detachment and lysing. Endocytosed FN was quantified with the Quantikine ELISA kit. USP10 overexpression induced an increase internalized fibronectin fold change (2.1; P < 0.05, Student's t -test). Two primary cell lines (total N = 3 repeats). Five images per condition/per experiment were analyzed.
    Usp10 (Nm 005153) Human Tagged Orf Clone, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usp10+cdna/USP10+(NM_005153)+Human+Tagged+ORF+Clone/origene___rc200835
    Average 90 stars, based on 1 article reviews
    usp10 (nm_005153) human tagged orf clone - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    usp10 cdna  (Addgene inc)
    93
    Addgene inc usp10 cdna
    <t>USP10</t> overexpression promoted integrin recycling and fibronectin endocytosis. ( A – F ) Live cell integrin recycling assay. HCFs were transfected with 2ug control or USP10 cDNA. 48 hours post transfection, integrin recycling was detected with anti-rabbit-488 antibody prior to imaging by live cell confocal microscopy. Scale bar : 50 µm. ( C ) Fluorescence intensity of α5β1 integrin recycling fold change (1.9; P < 0.001) and ( F ) αv (1.7; P < 0.05) when USP10 was overexpressed. ( G ) FN ELISA. HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours prior to detachment and lysing. Endocytosed FN was quantified with the Quantikine ELISA kit. USP10 overexpression induced an increase internalized fibronectin fold change (2.1; P < 0.05, Student's t -test). Two primary cell lines (total N = 3 repeats). Five images per condition/per experiment were analyzed.
    Usp10 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usp10+cdna/Flag-HA-USP10+(Plasmid+%2322543)/pmc05665446-595-0-9
    Average 93 stars, based on 1 article reviews
    usp10 cdna - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    usp10 cdna  (OriGene)
    90
    OriGene usp10 cdna
    <t>USP10</t> overexpression promoted integrin recycling and fibronectin endocytosis. ( A – F ) Live cell integrin recycling assay. HCFs were transfected with 2ug control or USP10 cDNA. 48 hours post transfection, integrin recycling was detected with anti-rabbit-488 antibody prior to imaging by live cell confocal microscopy. Scale bar : 50 µm. ( C ) Fluorescence intensity of α5β1 integrin recycling fold change (1.9; P < 0.001) and ( F ) αv (1.7; P < 0.05) when USP10 was overexpressed. ( G ) FN ELISA. HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours prior to detachment and lysing. Endocytosed FN was quantified with the Quantikine ELISA kit. USP10 overexpression induced an increase internalized fibronectin fold change (2.1; P < 0.05, Student's t -test). Two primary cell lines (total N = 3 repeats). Five images per condition/per experiment were analyzed.
    Usp10 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usp10+cdna/USP10+(NM_005153)+Human+Tagged+ORF+Clone/10__1080_slash_2162402x__2018__1431086-161-3-8
    Average 90 stars, based on 1 article reviews
    usp10 cdna - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    USP10 overexpression promoted integrin recycling and fibronectin endocytosis. ( A – F ) Live cell integrin recycling assay. HCFs were transfected with 2ug control or USP10 cDNA. 48 hours post transfection, integrin recycling was detected with anti-rabbit-488 antibody prior to imaging by live cell confocal microscopy. Scale bar : 50 µm. ( C ) Fluorescence intensity of α5β1 integrin recycling fold change (1.9; P < 0.001) and ( F ) αv (1.7; P < 0.05) when USP10 was overexpressed. ( G ) FN ELISA. HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours prior to detachment and lysing. Endocytosed FN was quantified with the Quantikine ELISA kit. USP10 overexpression induced an increase internalized fibronectin fold change (2.1; P < 0.05, Student's t -test). Two primary cell lines (total N = 3 repeats). Five images per condition/per experiment were analyzed.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: USP10 Promotes Fibronectin Recycling, Secretion, and Organization

    doi: 10.1167/iovs.62.13.15

    Figure Lengend Snippet: USP10 overexpression promoted integrin recycling and fibronectin endocytosis. ( A – F ) Live cell integrin recycling assay. HCFs were transfected with 2ug control or USP10 cDNA. 48 hours post transfection, integrin recycling was detected with anti-rabbit-488 antibody prior to imaging by live cell confocal microscopy. Scale bar : 50 µm. ( C ) Fluorescence intensity of α5β1 integrin recycling fold change (1.9; P < 0.001) and ( F ) αv (1.7; P < 0.05) when USP10 was overexpressed. ( G ) FN ELISA. HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours prior to detachment and lysing. Endocytosed FN was quantified with the Quantikine ELISA kit. USP10 overexpression induced an increase internalized fibronectin fold change (2.1; P < 0.05, Student's t -test). Two primary cell lines (total N = 3 repeats). Five images per condition/per experiment were analyzed.

    Article Snippet: HCFs (1,000,000) were transfected with 2 mg control FLAG and USP10 FLAG cDNA (Sigma-Aldrich).

    Techniques: Over Expression, Transfection, Imaging, Confocal Microscopy, Fluorescence, Enzyme-linked Immunosorbent Assay

    USP10 overexpression increased FN recycling. For the live cell FN recycling assay, HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours. After trypsinization, cells were replated for days 1 to 4. FN recycling was detected with streptavidin-488 by live cell confocal microscopy. ( A ) Images from days 1 to 4. Scale bar : 50 µm. ( B ) Quantification of puncta count; USP10 overexpressing cells increased FN recycling (1.7–2.2-fold). Two-way ANOVA, two primary cell lines, total N = 6 repeats, five images per condition/per experiment. One dot represents the average of quantification of images in one experiment. ( C ) Average number of cells quantified per image were similar; Student's t -test.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: USP10 Promotes Fibronectin Recycling, Secretion, and Organization

    doi: 10.1167/iovs.62.13.15

    Figure Lengend Snippet: USP10 overexpression increased FN recycling. For the live cell FN recycling assay, HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection, HCFs were loaded with biotinylated FN for 3 hours. After trypsinization, cells were replated for days 1 to 4. FN recycling was detected with streptavidin-488 by live cell confocal microscopy. ( A ) Images from days 1 to 4. Scale bar : 50 µm. ( B ) Quantification of puncta count; USP10 overexpressing cells increased FN recycling (1.7–2.2-fold). Two-way ANOVA, two primary cell lines, total N = 6 repeats, five images per condition/per experiment. One dot represents the average of quantification of images in one experiment. ( C ) Average number of cells quantified per image were similar; Student's t -test.

    Article Snippet: HCFs (1,000,000) were transfected with 2 mg control FLAG and USP10 FLAG cDNA (Sigma-Aldrich).

    Techniques: Over Expression, Transfection, Confocal Microscopy

    Blocking integrins reduced FN recycling. ( A – C ) Live cell FN recycling assay. HCFs were transfected with 2 µg USP10 cDNA for 24 hours prior to treatment with control IgG, α5β1 integrin-blocking antibody, or αv integrin-blocking antibody. After 1 hour with antibodies, cells were treated with biotinylated FN for 3 hours prior to trypsinization and replating for 2 days. Scale bar : 50 µm. ( D ) Quantification. USP10 overexpressing cells treated with control IgG compared with α5β1 blocking antibody reduced FN recycling by 62% ( P < 0.05), and IgG compared with αv blocking antibody reduced FN recycling by 84% ( P < 0.05). One-way ANOVA, two primary cell lines, total N = 4 repeats, five images per condition/per experiment were analyzed.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: USP10 Promotes Fibronectin Recycling, Secretion, and Organization

    doi: 10.1167/iovs.62.13.15

    Figure Lengend Snippet: Blocking integrins reduced FN recycling. ( A – C ) Live cell FN recycling assay. HCFs were transfected with 2 µg USP10 cDNA for 24 hours prior to treatment with control IgG, α5β1 integrin-blocking antibody, or αv integrin-blocking antibody. After 1 hour with antibodies, cells were treated with biotinylated FN for 3 hours prior to trypsinization and replating for 2 days. Scale bar : 50 µm. ( D ) Quantification. USP10 overexpressing cells treated with control IgG compared with α5β1 blocking antibody reduced FN recycling by 62% ( P < 0.05), and IgG compared with αv blocking antibody reduced FN recycling by 84% ( P < 0.05). One-way ANOVA, two primary cell lines, total N = 4 repeats, five images per condition/per experiment were analyzed.

    Article Snippet: HCFs (1,000,000) were transfected with 2 mg control FLAG and USP10 FLAG cDNA (Sigma-Aldrich).

    Techniques: Blocking Assay, Transfection

    Ratio of total versus recycled FN for live cell FN recycling/secretion assay. ( A – D ) HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection HCFs were loaded with biotinylated FN for 3 hours. After trypsinization, cells were replated for 2 days. ( A , C ) FN recycling was detected with streptavidin-488 by live cell confocal microscopy. ( B , D ) Secreted FN-EDA was detected with FN-EDA-488 (colored red for image). Scale bars : 50 µm. ( E ) Quantification of recycled FN in control cells compared with USP10 overexpressing cells (1.79-fold; P < 0.05). Quantification of secreted FN-EDA in control cells compared with USP10 overexpressing cells (2.18-fold; P < 0.05). ( F ) The percentage of recycled FN and secreted FN-EDA for control cells versus USP10 overexpressing cells was not significantly different. One-way ANOVA, two primary cell lines, total N = 4 repeats, five images per condition/per experiment were analyzed.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: USP10 Promotes Fibronectin Recycling, Secretion, and Organization

    doi: 10.1167/iovs.62.13.15

    Figure Lengend Snippet: Ratio of total versus recycled FN for live cell FN recycling/secretion assay. ( A – D ) HCFs were transfected with 2 µg control or USP10 cDNA. Twenty-four hours after transfection HCFs were loaded with biotinylated FN for 3 hours. After trypsinization, cells were replated for 2 days. ( A , C ) FN recycling was detected with streptavidin-488 by live cell confocal microscopy. ( B , D ) Secreted FN-EDA was detected with FN-EDA-488 (colored red for image). Scale bars : 50 µm. ( E ) Quantification of recycled FN in control cells compared with USP10 overexpressing cells (1.79-fold; P < 0.05). Quantification of secreted FN-EDA in control cells compared with USP10 overexpressing cells (2.18-fold; P < 0.05). ( F ) The percentage of recycled FN and secreted FN-EDA for control cells versus USP10 overexpressing cells was not significantly different. One-way ANOVA, two primary cell lines, total N = 4 repeats, five images per condition/per experiment were analyzed.

    Article Snippet: HCFs (1,000,000) were transfected with 2 mg control FLAG and USP10 FLAG cDNA (Sigma-Aldrich).

    Techniques: Transfection, Confocal Microscopy

    Working model for USP10-mediated matrix deposition during wound healing Wounding initiates cell stress pathways. USP10 expression is increased after wounding, reducing integrin degradation through ubiquitin removal. , This shift promotes integrin recycling to the cell surface with endocytosed matrix ( green ). The increase in USP10-mediated integrin accumulation also leads to activation of TGFβ signaling, inducing FN-EDA secretion ( blue ) and the organization of α-SMA–containing stress fibers ( red ).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: USP10 Promotes Fibronectin Recycling, Secretion, and Organization

    doi: 10.1167/iovs.62.13.15

    Figure Lengend Snippet: Working model for USP10-mediated matrix deposition during wound healing Wounding initiates cell stress pathways. USP10 expression is increased after wounding, reducing integrin degradation through ubiquitin removal. , This shift promotes integrin recycling to the cell surface with endocytosed matrix ( green ). The increase in USP10-mediated integrin accumulation also leads to activation of TGFβ signaling, inducing FN-EDA secretion ( blue ) and the organization of α-SMA–containing stress fibers ( red ).

    Article Snippet: HCFs (1,000,000) were transfected with 2 mg control FLAG and USP10 FLAG cDNA (Sigma-Aldrich).

    Techniques: Expressing, Activation Assay