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Kreatech Diagnostics uls ® microrna labeling kit
Uls ® Microrna Labeling Kit, supplied by Kreatech Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uls+labeling/mirna+ulstm+labeling+kit/pmc11432929-185-18-23
Average 90 stars, based on 1 article reviews
uls ® microrna labeling kit - by Bioz Stars, 2026-11
90/100 stars

Images

Related Articles

Microarray:

Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication.
Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Kreatech ULS RNA Labeling Kit (Kreatech Diagnostics) and purified with Qiagen PCR purification columns. cDNA samples were hybridized to Agilent Mouse_v1 8 x 60K microarrays (Design ID-028005, Agilent Technologies). .. Gridding and analysis of images were performed using Feature Extraction software (v11.5.1.1, Agilent Technologies).

Amplification:

Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication.
Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Kreatech ULS RNA Labeling Kit (Kreatech Diagnostics) and purified with Qiagen PCR purification columns. cDNA samples were hybridized to Agilent Mouse_v1 8 x 60K microarrays (Design ID-028005, Agilent Technologies). .. Gridding and analysis of images were performed using Feature Extraction software (v11.5.1.1, Agilent Technologies).

Labeling:

Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication.
Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Kreatech ULS RNA Labeling Kit (Kreatech Diagnostics) and purified with Qiagen PCR purification columns. cDNA samples were hybridized to Agilent Mouse_v1 8 x 60K microarrays (Design ID-028005, Agilent Technologies). .. Gridding and analysis of images were performed using Feature Extraction software (v11.5.1.1, Agilent Technologies).

Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803
Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m 2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the ULS labeling kit (Kreatech Diagnostics) according to the manufacturer's protocol. .. RNA fragmentation and hybridization for Agilent one‐color microarrays were performed according to the manufacturer's instructions using 1.65 μg of labeled RNA in biological duplicates.

Article Title: Murine fecal microRNAs alter the composition of cultured gut microbiota.
Article Snippet: Fecal microRNAs (miRNAs) derived from intestinal epithelial cells have been suggested to influence gut microbiota homeostasis.. The present study examined whether fecal miRNAs alter the structure of cultured gut microbiota.. Fecal bacteria isolated from murine cecal contents were cultured for 24 h under anaerobic conditions.

Article Title: The Gene Expression Profile in the Synovium as a Predictor of the Clinical Response to Infliximab Treatment in Rheumatoid Arthritis
Article Snippet: .. Labeling was performed with the ULS aRNA Fluorescent Labeling Kit (Kreatech Diagnostics, the Netherlands) that allows for labeling of unmodified aRNA. ..

Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803
Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the ULS labeling kit (Kreatech Diagnostics) according to the manufacturer's protocol. .. RNA fragmentation and hybridization for Agilent one‐color microarrays were performed according to the manufacturer's instructions using 1.65 μg of labeled RNA in biological duplicates.

Purification:

Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication.
Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Kreatech ULS RNA Labeling Kit (Kreatech Diagnostics) and purified with Qiagen PCR purification columns. cDNA samples were hybridized to Agilent Mouse_v1 8 x 60K microarrays (Design ID-028005, Agilent Technologies). .. Gridding and analysis of images were performed using Feature Extraction software (v11.5.1.1, Agilent Technologies).

Polymerase Chain Reaction:

Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication.
Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Kreatech ULS RNA Labeling Kit (Kreatech Diagnostics) and purified with Qiagen PCR purification columns. cDNA samples were hybridized to Agilent Mouse_v1 8 x 60K microarrays (Design ID-028005, Agilent Technologies). .. Gridding and analysis of images were performed using Feature Extraction software (v11.5.1.1, Agilent Technologies).

other:

Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing
Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

Article Title: Induction of Olfaction and Cancer-Related Genes in Mice Fed a High-Fat Diet as Assessed through the Mode-of-Action by Network Identification Analysis
Article Snippet: For analysis, 2 μg total RNA was labeled and amplified using the Universal Linkage System antisense RNA (aRNA) labeling kit (Kreatech Diagnostics, Amsterdam, The Netherlands).

Article Title: Circulating microRNA signatures in mice exposed to lipoteichoic acid
Article Snippet: Mouse genome-wide miRNA microarray analysis was performed by Phalanx Biotech with the Mouse & Rat miRNA OneArray 1.0 (Phalanx Biotech Group, Hsinchu, Taiwan) contains a total of 2,319 probes, including 135 experimental control probes and 728 unique miRNA probes from mouse and 348 from rat (miRBase Release 12.0).

Irradiation:

Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803
Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m 2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the ULS labeling kit (Kreatech Diagnostics) according to the manufacturer's protocol. .. RNA fragmentation and hybridization for Agilent one‐color microarrays were performed according to the manufacturer's instructions using 1.65 μg of labeled RNA in biological duplicates.

Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803
Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the ULS labeling kit (Kreatech Diagnostics) according to the manufacturer's protocol. .. RNA fragmentation and hybridization for Agilent one‐color microarrays were performed according to the manufacturer's instructions using 1.65 μg of labeled RNA in biological duplicates.

cDNA Synthesis:

Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803
Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m 2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the ULS labeling kit (Kreatech Diagnostics) according to the manufacturer's protocol. .. RNA fragmentation and hybridization for Agilent one‐color microarrays were performed according to the manufacturer's instructions using 1.65 μg of labeled RNA in biological duplicates.

Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803
Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the ULS labeling kit (Kreatech Diagnostics) according to the manufacturer's protocol. .. RNA fragmentation and hybridization for Agilent one‐color microarrays were performed according to the manufacturer's instructions using 1.65 μg of labeled RNA in biological duplicates.

Isolation:

Article Title: Murine fecal microRNAs alter the composition of cultured gut microbiota.
Article Snippet: Fecal microRNAs (miRNAs) derived from intestinal epithelial cells have been suggested to influence gut microbiota homeostasis.. The present study examined whether fecal miRNAs alter the structure of cultured gut microbiota.. Fecal bacteria isolated from murine cecal contents were cultured for 24 h under anaerobic conditions.



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<t>DNA</t> extracted from <t>FFPE</t> leiomyosarcoma samples of different ages (shown in brackets) showing varied degrees of degradation, compared with commercial pooled female genomic DNA. B: Comparison of DNA extracted from paired FF and FFPE leiomyosarcoma samples (LMS 9, 10 and 11). FF samples show relatively distinct bands of high molecular weight, while corresponding FFPE samples show low molecular weight fragments in a wide range of sizes. All DNA samples are compared against a 1 Kb DNA ladder. DNA Electrophoresis was done on 1.0% agarose gels were pre-stained with Ethidium Bromide and examined under UV light.
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Image Search Results


DNA extracted from FFPE leiomyosarcoma samples of different ages (shown in brackets) showing varied degrees of degradation, compared with commercial pooled female genomic DNA. B: Comparison of DNA extracted from paired FF and FFPE leiomyosarcoma samples (LMS 9, 10 and 11). FF samples show relatively distinct bands of high molecular weight, while corresponding FFPE samples show low molecular weight fragments in a wide range of sizes. All DNA samples are compared against a 1 Kb DNA ladder. DNA Electrophoresis was done on 1.0% agarose gels were pre-stained with Ethidium Bromide and examined under UV light.

Journal: PLoS ONE

Article Title: High Quality Genomic Copy Number Data from Archival Formalin-Fixed Paraffin-Embedded Leiomyosarcoma: Optimisation of Universal Linkage System Labelling

doi: 10.1371/journal.pone.0050415

Figure Lengend Snippet: DNA extracted from FFPE leiomyosarcoma samples of different ages (shown in brackets) showing varied degrees of degradation, compared with commercial pooled female genomic DNA. B: Comparison of DNA extracted from paired FF and FFPE leiomyosarcoma samples (LMS 9, 10 and 11). FF samples show relatively distinct bands of high molecular weight, while corresponding FFPE samples show low molecular weight fragments in a wide range of sizes. All DNA samples are compared against a 1 Kb DNA ladder. DNA Electrophoresis was done on 1.0% agarose gels were pre-stained with Ethidium Bromide and examined under UV light.

Article Snippet: FFPE tumour and reference DNA was labelled using an optimised version of the protocol for ULS labelling of FFPE DNA (Agilent®).

Techniques: Molecular Weight, Nucleic Acid Electrophoresis, Staining