Journal: Scientific Reports

Article Title: Evaluation of nitrogen fixation in the marine purple photosynthetic bacterium Rhodovulum sulfidophilum under autotrophic and heterotrophic conditions

doi: 10.1038/s41598-025-03605-4

Figure Lengend Snippet: Effects of the carbon source on the growth and nitrogenase activity of R. sulfidophilum . (a) Growth of R. sulfidophilum when malate (white circle) or NaHCO 3 (black circle) was used as a carbon source in M6-mineral medium. (b) Nitrogenase activity of R. sulfidophilum cultures supplemented with malate and NaHCO 3 using acetylene as the substrate. These data are the averages from three independent experiments, and the error bars represent the SDs. Asterisks indicate statistically significant differences with ** P < 0.01 and *** P < 0.001 as determined by Student’s t test.

Article Snippet: R. sulfidophilum (ATCC35886/DSM1374) was obtained from the American Type Culture Collection (ATCC).

Techniques: Activity Assay