Review



human glioblastoma u87 mg cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC human glioblastoma u87 mg cells
    Human Glioblastoma U87 Mg Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pm42278448-476-33-39
    Average 99 stars, based on 10584 article reviews
    human glioblastoma u87 mg cells - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER L-Tyrosine Sigma-Aldrich Cat #: T8566 L-Valine Sigma-Aldrich Cat #: V0513 Bromophenol blue sodium salt Sigma-Aldrich Cat #: B8026 Methanol Thermo Fisher Scientific Cat #: BPA412P4 Lenti-X Concentrator Clontech Cat #: 631231 TRIzolTM Reagent Invitrogen Cat #:15596018 TWEEN 80 Sigma-Aldrich Cat #:P8074 Critical commercial assays PowerUpTM SYBRTM Green Master Mix Applied Biosystems Cat #: A25778 Qproteome Mitochondria Isolation Kit QIAGEN Cat #: 37612 LysoSensorTM Yellow/Blue dextran Thermo Fisher Scientific Cat #: L22460 DQ-green BSA ThermoFisher Scientific Cat #: D12050 BODIPYTM 493/503 Thermo Fisher Scientific Cat #: D3922 LysoTrackerTM Red DND-99 Thermo Fisher Scientific Cat #: L7528 Low-Density Lipoprotein from Human Plasma, BODIPYTM FL-LDL complex Thermo Fisher Scientific Cat #: L3483 Rhodamine 123 Thermo Fisher Scientific Cat #: R302 CellROXTM Deep Red reagent Thermo Fisher Scientific Cat #: C10422 MitoTrackerTM Green FM Dye Thermo Fisher Scientific Cat #: M7514 Free fatty acid assay Kit (Quantification) Abcam Cat #: ab65341 Cardiolipin assay Kit Sigma-Aldrich Cat #: MAK362 Amplex Red Cholesterol Assay Kit Invitrogen Cat #: A12216 Glutaminase (GLS) Activity Assay Kit (Fluorometric) Abcam Cat #: ab284547 BCA protein assay kit II Abcam Cat #: ab287853 RIPA lysis buffer Thermo Fisher Scientific Cat #: NC9484499 EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Cat #: 11836170001 Phosphatase inhibitor Sigma-Aldrich Cat #: 4906837001 Hoechst 33342 Solution Thermo Fisher Scientific Cat #: 62249 Glo Lysis buffer Promega Cat #: E266A Promega Renilla-GloTM Luciferase Assay System Thermo Fisher Scientific Cat #: PRE2710 X-tremeGENETM HP DNA Transfection Reagent Sigma-Aldrich Cat #: 6366236001 ECL kit Cytiva Amersham Cat #: RPN2106 iScript cDNA Synthesis Kit Bio-rad Cat #: 170-8891 Deposited data Metabolomics data This paper NMDR: ST003362 Project DOI: https://doi.org/10.21228/M8QJ9B Experimental models: Cell lines GBM patient-derived primary cell, GBM30 The Ohio State University N/A GBM patient-derived primary cell, GBM6 Mayo clinic N/A GBM patient-derived primary cell, GBM26 Mayo clinic N/A Human GBM cell: U251 Sigma-Aldrich Cat #: 09063001; Human GBM cell: U87 Sigma-Aldrich Cat #: 89081402; Human GBM cell: U373 Sigma-Aldrich Cat #: 08061901; Human GBM cell: T98 ATCC Cat #: #CRL-1690TM ATCC; Human GBM cell: LN229 ATCC Cat #: CRL-2611TM; Human embryonal kidney cell: 293FT InvitrogenTM Cat #: R70007 Experimental models: Organisms/strains Athymic NCr-nu/nu, outbred, NCI stock Charles River Lab Strain # 553 (Continued on next page) e3 Cell Reports Medicine 5, 101706, September 17, 2024 OPEN ACCESS .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..

    Article Title: Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system
    Article Snippet: .. Four cell lines, HeLa (CRM-CCL-2), HS27 (CRL-1634), U87 (HTB-14) and Caco-2 (HTB-37) were procured from the American Type Culture Collection (ATCC) and cultured adherently in Dulbecco’s Modified Eagle’s Medium (DMEM). .. The culture medium was supplemented with 1% glutamine (Wuhan Pricella Bi Co., Ltd., Hubei, China), 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, United States), and 1% penicillin–streptomycin solution (Wuhan Pricella Bio Co., Ltd., Hubei, China) ( ).

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma
    Article Snippet: .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..

    Modification:

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER L-Tyrosine Sigma-Aldrich Cat #: T8566 L-Valine Sigma-Aldrich Cat #: V0513 Bromophenol blue sodium salt Sigma-Aldrich Cat #: B8026 Methanol Thermo Fisher Scientific Cat #: BPA412P4 Lenti-X Concentrator Clontech Cat #: 631231 TRIzolTM Reagent Invitrogen Cat #:15596018 TWEEN 80 Sigma-Aldrich Cat #:P8074 Critical commercial assays PowerUpTM SYBRTM Green Master Mix Applied Biosystems Cat #: A25778 Qproteome Mitochondria Isolation Kit QIAGEN Cat #: 37612 LysoSensorTM Yellow/Blue dextran Thermo Fisher Scientific Cat #: L22460 DQ-green BSA ThermoFisher Scientific Cat #: D12050 BODIPYTM 493/503 Thermo Fisher Scientific Cat #: D3922 LysoTrackerTM Red DND-99 Thermo Fisher Scientific Cat #: L7528 Low-Density Lipoprotein from Human Plasma, BODIPYTM FL-LDL complex Thermo Fisher Scientific Cat #: L3483 Rhodamine 123 Thermo Fisher Scientific Cat #: R302 CellROXTM Deep Red reagent Thermo Fisher Scientific Cat #: C10422 MitoTrackerTM Green FM Dye Thermo Fisher Scientific Cat #: M7514 Free fatty acid assay Kit (Quantification) Abcam Cat #: ab65341 Cardiolipin assay Kit Sigma-Aldrich Cat #: MAK362 Amplex Red Cholesterol Assay Kit Invitrogen Cat #: A12216 Glutaminase (GLS) Activity Assay Kit (Fluorometric) Abcam Cat #: ab284547 BCA protein assay kit II Abcam Cat #: ab287853 RIPA lysis buffer Thermo Fisher Scientific Cat #: NC9484499 EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Cat #: 11836170001 Phosphatase inhibitor Sigma-Aldrich Cat #: 4906837001 Hoechst 33342 Solution Thermo Fisher Scientific Cat #: 62249 Glo Lysis buffer Promega Cat #: E266A Promega Renilla-GloTM Luciferase Assay System Thermo Fisher Scientific Cat #: PRE2710 X-tremeGENETM HP DNA Transfection Reagent Sigma-Aldrich Cat #: 6366236001 ECL kit Cytiva Amersham Cat #: RPN2106 iScript cDNA Synthesis Kit Bio-rad Cat #: 170-8891 Deposited data Metabolomics data This paper NMDR: ST003362 Project DOI: https://doi.org/10.21228/M8QJ9B Experimental models: Cell lines GBM patient-derived primary cell, GBM30 The Ohio State University N/A GBM patient-derived primary cell, GBM6 Mayo clinic N/A GBM patient-derived primary cell, GBM26 Mayo clinic N/A Human GBM cell: U251 Sigma-Aldrich Cat #: 09063001; Human GBM cell: U87 Sigma-Aldrich Cat #: 89081402; Human GBM cell: U373 Sigma-Aldrich Cat #: 08061901; Human GBM cell: T98 ATCC Cat #: #CRL-1690TM ATCC; Human GBM cell: LN229 ATCC Cat #: CRL-2611TM; Human embryonal kidney cell: 293FT InvitrogenTM Cat #: R70007 Experimental models: Organisms/strains Athymic NCr-nu/nu, outbred, NCI stock Charles River Lab Strain # 553 (Continued on next page) e3 Cell Reports Medicine 5, 101706, September 17, 2024 OPEN ACCESS .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..

    Article Title: Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system
    Article Snippet: .. Four cell lines, HeLa (CRM-CCL-2), HS27 (CRL-1634), U87 (HTB-14) and Caco-2 (HTB-37) were procured from the American Type Culture Collection (ATCC) and cultured adherently in Dulbecco’s Modified Eagle’s Medium (DMEM). .. The culture medium was supplemented with 1% glutamine (Wuhan Pricella Bi Co., Ltd., Hubei, China), 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, United States), and 1% penicillin–streptomycin solution (Wuhan Pricella Bio Co., Ltd., Hubei, China) ( ).

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma
    Article Snippet: .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..



    Similar Products

    99
    ATCC human glioblastoma u87 mg cells
    Human Glioblastoma U87 Mg Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pm42278448-476-33-39
    Average 99 stars, based on 1 article reviews
    human glioblastoma u87 mg cells - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    ATCC u87 mg htb 14 cell line
    U87 Mg Htb 14 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pm42278615-241-18-26
    Average 99 stars, based on 1 article reviews
    u87 mg htb 14 cell line - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    ATCC human glioblastoma cell line u87 mg
    Human Glioblastoma Cell Line U87 Mg, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pm42280247-383-1-12
    Average 99 stars, based on 1 article reviews
    human glioblastoma cell line u87 mg - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    u87  (ATCC)
    99
    ATCC u87
    U87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pm42285982-205-0-2
    Average 99 stars, based on 1 article reviews
    u87 - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    ATCC u87 glioblastoma cells
    U87 Glioblastoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/us12649792-922-29-32
    Average 99 stars, based on 1 article reviews
    u87 glioblastoma cells - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    ATCC human gbm cell lines u87 mg u87 mg
    Human Gbm Cell Lines U87 Mg U87 Mg, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pm42252325-167-0-7
    Average 99 stars, based on 1 article reviews
    human gbm cell lines u87 mg u87 mg - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    ATCC u87 mg cells
    U87 Mg Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/us12643899-503-14-17
    Average 99 stars, based on 1 article reviews
    u87 mg cells - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    ATCC glioma cells u87 mg
    ZNF25 knockdown inhibits proliferation and migration of A172 <t>and</t> <t>U87-MG</t> Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).
    Glioma Cells U87 Mg, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pmc13268941-128-0-7
    Average 99 stars, based on 1 article reviews
    glioma cells u87 mg - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    ATCC u87 mg cell line
    ZNF25 knockdown inhibits proliferation and migration of A172 <t>and</t> <t>U87-MG</t> Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).
    U87 Mg Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pm42230717-78-0-4
    Average 99 stars, based on 1 article reviews
    u87 mg cell line - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    ZNF25 knockdown inhibits proliferation and migration of A172 and U87-MG Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: Frontiers in Oncology

    Article Title: ZNF25 as an immunotherapy target: pan-cancer biomarker potential and mechanistic exploration in glioma

    doi: 10.3389/fonc.2026.1631383

    Figure Lengend Snippet: ZNF25 knockdown inhibits proliferation and migration of A172 and U87-MG Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: Glioma cells U87-MG were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and A172 were purchased from Xiamen Immocell Biotechnology Co. Ltd.

    Techniques: Knockdown, Migration, Quantitative RT-PCR, Western Blot, Transfection, Control, Expressing, Software, CCK-8 Assay

    Functional enrichment analysis and molecular verification of ZNF25 knockdown. (A) The volcano plot visualized all identified DEGs in U87-MG cells, which were screened by the criteria of adjusted P-value (padj) < 0.05 and ∣log 2 FC∣>1. (B) Top 10 enriched GO terms across all categories. (C) Top 10 enriched KEGG pathways. (D) Western blot analysis of p-AKT protein levels in A172 and U87-MG cells at 72h after transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in U87-MG cells corresponding to (D) . Data are presented as mean ± SD (n = 3 independent experiment, Student’s t-test). (* P < 0.05, ** P < 0.01).

    Journal: Frontiers in Oncology

    Article Title: ZNF25 as an immunotherapy target: pan-cancer biomarker potential and mechanistic exploration in glioma

    doi: 10.3389/fonc.2026.1631383

    Figure Lengend Snippet: Functional enrichment analysis and molecular verification of ZNF25 knockdown. (A) The volcano plot visualized all identified DEGs in U87-MG cells, which were screened by the criteria of adjusted P-value (padj) < 0.05 and ∣log 2 FC∣>1. (B) Top 10 enriched GO terms across all categories. (C) Top 10 enriched KEGG pathways. (D) Western blot analysis of p-AKT protein levels in A172 and U87-MG cells at 72h after transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in U87-MG cells corresponding to (D) . Data are presented as mean ± SD (n = 3 independent experiment, Student’s t-test). (* P < 0.05, ** P < 0.01).

    Article Snippet: Glioma cells U87-MG were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and A172 were purchased from Xiamen Immocell Biotechnology Co. Ltd.

    Techniques: Functional Assay, Knockdown, Western Blot, Transfection, Control, Expressing