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human glioblastoma u87 mg cells  (ATCC)


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    Structured Review

    ATCC human glioblastoma u87 mg cells
    Human Glioblastoma U87 Mg Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+cells/U-87+MG/pm42278448-476-33-39
    Average 99 stars, based on 10546 article reviews
    human glioblastoma u87 mg cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.
    Article Snippet: .. U87 cells (ATCC, HTB-14) were plated in DMEM+ in 6-well plates 24 hours before transduction and infected at 70-90% confluence with 500 μL lentiviral supernatant per well in the presence of 10 μg/mL polybrene (Millipore Sigma #TR1003), followed by spinfection for 50 min at 35°C and incubation for 16-20 hours at 37°C. ..

    Infection:

    Article Title: Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.
    Article Snippet: .. U87 cells (ATCC, HTB-14) were plated in DMEM+ in 6-well plates 24 hours before transduction and infected at 70-90% confluence with 500 μL lentiviral supernatant per well in the presence of 10 μg/mL polybrene (Millipore Sigma #TR1003), followed by spinfection for 50 min at 35°C and incubation for 16-20 hours at 37°C. ..

    Incubation:

    Article Title: Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.
    Article Snippet: .. U87 cells (ATCC, HTB-14) were plated in DMEM+ in 6-well plates 24 hours before transduction and infected at 70-90% confluence with 500 μL lentiviral supernatant per well in the presence of 10 μg/mL polybrene (Millipore Sigma #TR1003), followed by spinfection for 50 min at 35°C and incubation for 16-20 hours at 37°C. ..

    Stable Transfection:

    Article Title: Bispecific antibody molecules binding to CD3 and EGFRvIII
    Article Snippet: .. The EGFRvIII TCB (comprising the optimized CD3 binder identified in Example 1) was likewise tested for its anti-tumoral efficacy in a xenograft mouse model of a human tumor cell line, the U87-EGFRvIII glioblastoma xenograft model. U87 cells (human glioblastoma) were originally obtained from ATCC (Manassas, USA) and stably transfected to express the human EGFRvIII protein (Roche Glycart AG, Switzerland). .. After expansion the cells were deposited in the Roche Glycart internal cell bank.

    Transfection:

    Article Title: Bispecific antibody molecules binding to CD3 and EGFRvIII
    Article Snippet: .. The EGFRvIII TCB (comprising the optimized CD3 binder identified in Example 1) was likewise tested for its anti-tumoral efficacy in a xenograft mouse model of a human tumor cell line, the U87-EGFRvIII glioblastoma xenograft model. U87 cells (human glioblastoma) were originally obtained from ATCC (Manassas, USA) and stably transfected to express the human EGFRvIII protein (Roche Glycart AG, Switzerland). .. After expansion the cells were deposited in the Roche Glycart internal cell bank.

    Cell Culture:

    Article Title: Tunable 3D‐Printed Static Mixers for Gradient Bioprinting With High Cell Viability
    Article Snippet: .. U87 cells (U-87 MG, ATCC HTB-14, LGC Standards GmbH, Germany) were cultured in Dulbecco’s Minimal Essential Medium (DMEM) (41966-029, Gibco, MA, USA) supplemented with 10% FCS (10270-106 Life Technologies, MA, USA), 0.5% Penicillin (50 U mL− 1 )/Streptomycin (50 μg mL− 1 ) (15140-122 Life Technologies). .. NIH3T3 cells (NIH/3T3, ATCC CRL-1658, ATCC, Manassas, VA, USA) were cultivated in Dulbecco’s Modified Eagle Medium + GlutaMAX-I [ + ] 4.5 g/L D-Glucose, [ + ] Pyruvate (DMEM) (31966-021, Gibco) supplemented with 10% BCS and 1% Penicillin/Streptomycin (AC-AB-0024, Anprotec, Bruckberg, Germany).

    Functional Assay:

    Article Title: Restoring the glioblastoma tumor microenvironment by targeting C5a with the antagonist W54011.
    Article Snippet: .. Lastly, although U87 cells were included under 352 tumorsphere-forming conditions to highlight functional differences between established glioma models and 353 patient-derived tumorspheres, the U87MG line—particularly the ATCC variant—is known to diverge 354 substantially from the primary tumor from which it was originally derived, both in genotype and phenotype. ..

    Article Title: Restoring the glioblastoma tumor microenvironment by targeting C5a with the antagonist W54011
    Article Snippet: .. Lastly, although U87 cells were included under tumorsphere-forming conditions to highlight functional differences between established glioma models and patient-derived tumorspheres, the U87MG line—particularly the ATCC variant—is known to diverge substantially from the primary tumor from which it was originally derived, both in genotype and phenotype. ..

    Variant Assay:

    Article Title: Restoring the glioblastoma tumor microenvironment by targeting C5a with the antagonist W54011.
    Article Snippet: .. Lastly, although U87 cells were included under 352 tumorsphere-forming conditions to highlight functional differences between established glioma models and 353 patient-derived tumorspheres, the U87MG line—particularly the ATCC variant—is known to diverge 354 substantially from the primary tumor from which it was originally derived, both in genotype and phenotype. ..

    Article Title: Restoring the glioblastoma tumor microenvironment by targeting C5a with the antagonist W54011
    Article Snippet: .. Lastly, although U87 cells were included under tumorsphere-forming conditions to highlight functional differences between established glioma models and patient-derived tumorspheres, the U87MG line—particularly the ATCC variant—is known to diverge substantially from the primary tumor from which it was originally derived, both in genotype and phenotype. ..

    Derivative Assay:

    Article Title: Restoring the glioblastoma tumor microenvironment by targeting C5a with the antagonist W54011.
    Article Snippet: .. Lastly, although U87 cells were included under 352 tumorsphere-forming conditions to highlight functional differences between established glioma models and 353 patient-derived tumorspheres, the U87MG line—particularly the ATCC variant—is known to diverge 354 substantially from the primary tumor from which it was originally derived, both in genotype and phenotype. ..

    Article Title: Restoring the glioblastoma tumor microenvironment by targeting C5a with the antagonist W54011
    Article Snippet: .. Lastly, although U87 cells were included under tumorsphere-forming conditions to highlight functional differences between established glioma models and patient-derived tumorspheres, the U87MG line—particularly the ATCC variant—is known to diverge substantially from the primary tumor from which it was originally derived, both in genotype and phenotype. ..

    Multiple Displacement Amplification:

    Article Title: OR7A10 GPCR engineering boosts CAR-NK therapy against solid tumours.
    Article Snippet: Chimeric antigen receptor (CAR)-natural killer (NK) cell therapies hold promise for solid tumours but remain limited because of poor tumour infiltration, persistence and resistance in the tumour microenvironment.. Here, to identify gain-of-function targets that enhance CAR-NK cell efficacy, we performed an unbiased in vivo CRISPR activation screen followed by a barcoded targeted in vivo open reading frame screen in primary human CAR-NK cells.. We identified and comprehensively validated OR7A10, a G protein-coupled receptor (GPCR), as the top candidate.



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    ATCC glioma cells u87 mg
    ZNF25 knockdown inhibits proliferation and migration of A172 <t>and</t> <t>U87-MG</t> Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).
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    Image Search Results


    ZNF25 knockdown inhibits proliferation and migration of A172 and U87-MG Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: Frontiers in Oncology

    Article Title: ZNF25 as an immunotherapy target: pan-cancer biomarker potential and mechanistic exploration in glioma

    doi: 10.3389/fonc.2026.1631383

    Figure Lengend Snippet: ZNF25 knockdown inhibits proliferation and migration of A172 and U87-MG Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: Glioma cells U87-MG were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and A172 were purchased from Xiamen Immocell Biotechnology Co. Ltd.

    Techniques: Knockdown, Migration, Quantitative RT-PCR, Western Blot, Transfection, Control, Expressing, Software, CCK-8 Assay

    Functional enrichment analysis and molecular verification of ZNF25 knockdown. (A) The volcano plot visualized all identified DEGs in U87-MG cells, which were screened by the criteria of adjusted P-value (padj) < 0.05 and ∣log 2 FC∣>1. (B) Top 10 enriched GO terms across all categories. (C) Top 10 enriched KEGG pathways. (D) Western blot analysis of p-AKT protein levels in A172 and U87-MG cells at 72h after transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in U87-MG cells corresponding to (D) . Data are presented as mean ± SD (n = 3 independent experiment, Student’s t-test). (* P < 0.05, ** P < 0.01).

    Journal: Frontiers in Oncology

    Article Title: ZNF25 as an immunotherapy target: pan-cancer biomarker potential and mechanistic exploration in glioma

    doi: 10.3389/fonc.2026.1631383

    Figure Lengend Snippet: Functional enrichment analysis and molecular verification of ZNF25 knockdown. (A) The volcano plot visualized all identified DEGs in U87-MG cells, which were screened by the criteria of adjusted P-value (padj) < 0.05 and ∣log 2 FC∣>1. (B) Top 10 enriched GO terms across all categories. (C) Top 10 enriched KEGG pathways. (D) Western blot analysis of p-AKT protein levels in A172 and U87-MG cells at 72h after transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in U87-MG cells corresponding to (D) . Data are presented as mean ± SD (n = 3 independent experiment, Student’s t-test). (* P < 0.05, ** P < 0.01).

    Article Snippet: Glioma cells U87-MG were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and A172 were purchased from Xiamen Immocell Biotechnology Co. Ltd.

    Techniques: Functional Assay, Knockdown, Western Blot, Transfection, Control, Expressing