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u20s cells  (ATCC)


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    Structured Review

    ATCC u20s cells
    U20s Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u20s+cells/U-2+OS/pmc12152113-24-0-2
    Average 99 stars, based on 8472 article reviews
    u20s cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Modification:

    Article Title: Human paraneoplastic antigen Ma2 (PNMA2) forms icosahedral capsids that can be engineered for mRNA delivery
    Article Snippet: The microscope was operated at 200 kV and with a magnification in the range of 10,000~60,000×, and all images were recorded on a Gatan 2kx2k UltraScan CCD camera. .. U20S cells (ATCC HTB-96) were maintained in McCoy’s 5A (Modified) Medium supplemented with 10% fetal bovine serum and 100 U/mL penicillin-streptomycin. .. HEK293FT (Thermo Fisher R700-07), HeLa (ATCC CCL-2), U87 (ATCC HTB-14), and Neuro2A (ATCC CCL-131) cells were maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum and 100 U/mL penicillin-streptomycin.

    Article Title: A live-cell fluorescence microplate assay suitable for monitoring vacuolation arising from drug or toxic agent treatment.
    Article Snippet: .. the Biomol brand iccB Known Bioactives library, lyso-id® red dye, and hoechst 33342 dye were obtained from enzo life sciences (plymouth meeting, pa). chloroquine, atropine, propranolol, verapamil, imipramine, fluoxetine, chlorpromazine, pimozide, dulbecco’s modified eagle’s medium (dmem) with high-glucose cell culture media, and fetal bovine serum (fBs) were obtained from sigma-aldrich chemical company (st. louis, mo). lipidtox reagent, sodium pyruvate, nonessential amino acids, and penicillin/ streptomycin solutions were obtained from invitrogen (carlsbad, ca). eagle’s minimum essential medium, mccoy’s 5a modified medium, hela cells (atcc no. ccl-2), mrc-5 (atcc no. ccl-171), and u20s cells (atcc no. htB-96) were obtained from the american type culture collection (manassas, Va). ..

    Transfection:

    Article Title: The Ewing's sarcoma protein interacts with the Tudor domain of the survival motor neuron protein.
    Article Snippet: The survival motor neuron (SMN) gene is the spinal muscular atrophy (SMA) determining gene.. Here we report that the SMN protein product interacts in vitro and in vivo with the arginine/glycine (RG)-rich RNA binding protein and transcription factor, Ewing’s sarcoma (EWS).. Recently, the SMN encoded Tudor domain (exon 3) and the YG-motifs (exon 6) have been shown to be involved in binding to RGrich proteins.

    Immunoprecipitation:

    Article Title: The Ewing's sarcoma protein interacts with the Tudor domain of the survival motor neuron protein.
    Article Snippet: The survival motor neuron (SMN) gene is the spinal muscular atrophy (SMA) determining gene.. Here we report that the SMN protein product interacts in vitro and in vivo with the arginine/glycine (RG)-rich RNA binding protein and transcription factor, Ewing’s sarcoma (EWS).. Recently, the SMN encoded Tudor domain (exon 3) and the YG-motifs (exon 6) have been shown to be involved in binding to RGrich proteins.

    Binding Assay:

    Article Title: The Ewing's sarcoma protein interacts with the Tudor domain of the survival motor neuron protein.
    Article Snippet: The survival motor neuron (SMN) gene is the spinal muscular atrophy (SMA) determining gene.. Here we report that the SMN protein product interacts in vitro and in vivo with the arginine/glycine (RG)-rich RNA binding protein and transcription factor, Ewing’s sarcoma (EWS).. Recently, the SMN encoded Tudor domain (exon 3) and the YG-motifs (exon 6) have been shown to be involved in binding to RGrich proteins.

    Cell Culture:

    Article Title: Method for determining cell cycle position
    Article Snippet: .. U20S cells (ATCC HTB-96) were cultured in wells of a 96 well microtitre plate. .. Cells were transfected with a cell cycle reporter construct prepared according to Example 1, comprising a cyclin B1 promoter operably linked to sequences encoding the cyclin B1 D-box, the cyclin B1 CRS, and GFP in a pCORON4004 vector (Amersham Biosciences) using Fugene 6 (Roche) as the transfection agent.

    Article Title: A live-cell fluorescence microplate assay suitable for monitoring vacuolation arising from drug or toxic agent treatment.
    Article Snippet: .. the Biomol brand iccB Known Bioactives library, lyso-id® red dye, and hoechst 33342 dye were obtained from enzo life sciences (plymouth meeting, pa). chloroquine, atropine, propranolol, verapamil, imipramine, fluoxetine, chlorpromazine, pimozide, dulbecco’s modified eagle’s medium (dmem) with high-glucose cell culture media, and fetal bovine serum (fBs) were obtained from sigma-aldrich chemical company (st. louis, mo). lipidtox reagent, sodium pyruvate, nonessential amino acids, and penicillin/ streptomycin solutions were obtained from invitrogen (carlsbad, ca). eagle’s minimum essential medium, mccoy’s 5a modified medium, hela cells (atcc no. ccl-2), mrc-5 (atcc no. ccl-171), and u20s cells (atcc no. htB-96) were obtained from the american type culture collection (manassas, Va). ..



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    Image Search Results


    SRI-22141 Displayed High Affinity, Potency, and Efficacy at the MOR and  DOR.  Affinity (K I ), potency (EC 50 ), and efficacy (E MAX ) values at the  MOR,  DOR, and KOR derived as reported in the Methods. The reported efficacy values were normalized to the stimulation caused by positive control compound (100%; SNC80 for DOR and DAMGO for MOR) and vehicle (0%). Data reported as the mean ± SEM from N = 3 independent experiments performed in duplicate. The positive control compounds (naloxone, U50,488, SNC80, DAMGO) returned expected values in each assay, validating the results.

    Journal: The journal of pain : official journal of the American Pain Society

    Article Title: A Novel Mu-Delta Opioid Agonist Demonstrates Enhanced Efficacy with Reduced Tolerance and Dependence in Mouse Neuropathic Pain Models

    doi: 10.1016/j.jpain.2019.05.017

    Figure Lengend Snippet: SRI-22141 Displayed High Affinity, Potency, and Efficacy at the MOR and DOR. Affinity (K I ), potency (EC 50 ), and efficacy (E MAX ) values at the MOR, DOR, and KOR derived as reported in the Methods. The reported efficacy values were normalized to the stimulation caused by positive control compound (100%; SNC80 for DOR and DAMGO for MOR) and vehicle (0%). Data reported as the mean ± SEM from N = 3 independent experiments performed in duplicate. The positive control compounds (naloxone, U50,488, SNC80, DAMGO) returned expected values in each assay, validating the results.

    Article Snippet: For the quantitative βarr2 recruitment assay using MOR- and DOR-U20S PathHunter cells from DiscoveRx, the manufacturer’s protocol was followed for all steps.

    Techniques: Derivative Assay, Positive Control, Binding Assay, Activity Assay