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human aml cell line u937  (ATCC)


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    Structured Review

    ATCC human aml cell line u937
    Human Aml Cell Line U937, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6873 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u+937/U-937/pmc13000501-55-1-10
    Average 99 stars, based on 6873 article reviews
    human aml cell line u937 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: DDX6 undergoes phase separation to modulate metabolic plasticity and chemoresistance
    Article Snippet: .. The human leukemia cells THP-1 (ATCC), NOMO-1 (DSMZ), MV-4-11 (ATCC), ML-2 (DSMZ), MOLM-13 (DSMZ), Kasumi-1 (ATCC), U-937 (ATCC), and HL-60 (ATCC) were cultured in RPMI1640 (11875119, Thermo Fisher Scientific) supplemented with 10% FBS (FB-11, Omega Scientific). .. OCI-AML3 (DSMZ) was maintained in MEM α, nucleosides (12571063, Thermo Fisher Scientific) supplemented with 10% FBS.

    Article Title: DDX6 undergoes phase separation to modulate metabolic plasticity and chemoresistance.
    Article Snippet: .. The human leukemia cells THP-1 (ATCC), NOMO-1 (DSMZ), MV-4-11 (ATCC), ML-2 (DSMZ), MOLM-13 (DSMZ), Kasumi-1 (ATCC), U-937 (ATCC), and HL-60 (ATCC) were cultured in RPMI1640 (11875119, Thermo Fisher Scientific) supplemented with 10% FBS (FB-11, Omega Scientific). .. OCI-AML3 (DSMZ) was maintained in MEM α, nucleosides (12571063, Thermo Fisher Scientific) supplemented with 10% FBS.

    Negative Control:

    Article Title: Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells.
    Article Snippet: .. A negative control experiment was performed by mating Y2h gold yeast stain fused with lamin and Y187 yeast strain fused with SV40 large T-antigen. cDNA synthesis and PCR amplification PPP2CA and AMBRA1 cDNAs were prepared using oligo primers by PCR using RNA extracted from U-937 (ATCC CRL-1593.2TM) and A-375 (ATCC CRL1619TM) cell lines. ..

    Staining:

    Article Title: Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells.
    Article Snippet: .. A negative control experiment was performed by mating Y2h gold yeast stain fused with lamin and Y187 yeast strain fused with SV40 large T-antigen. cDNA synthesis and PCR amplification PPP2CA and AMBRA1 cDNAs were prepared using oligo primers by PCR using RNA extracted from U-937 (ATCC CRL-1593.2TM) and A-375 (ATCC CRL1619TM) cell lines. ..

    cDNA Synthesis:

    Article Title: Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells.
    Article Snippet: .. A negative control experiment was performed by mating Y2h gold yeast stain fused with lamin and Y187 yeast strain fused with SV40 large T-antigen. cDNA synthesis and PCR amplification PPP2CA and AMBRA1 cDNAs were prepared using oligo primers by PCR using RNA extracted from U-937 (ATCC CRL-1593.2TM) and A-375 (ATCC CRL1619TM) cell lines. ..

    Polymerase Chain Reaction:

    Article Title: Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells.
    Article Snippet: .. A negative control experiment was performed by mating Y2h gold yeast stain fused with lamin and Y187 yeast strain fused with SV40 large T-antigen. cDNA synthesis and PCR amplification PPP2CA and AMBRA1 cDNAs were prepared using oligo primers by PCR using RNA extracted from U-937 (ATCC CRL-1593.2TM) and A-375 (ATCC CRL1619TM) cell lines. ..

    Amplification:

    Article Title: Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells.
    Article Snippet: .. A negative control experiment was performed by mating Y2h gold yeast stain fused with lamin and Y187 yeast strain fused with SV40 large T-antigen. cDNA synthesis and PCR amplification PPP2CA and AMBRA1 cDNAs were prepared using oligo primers by PCR using RNA extracted from U-937 (ATCC CRL-1593.2TM) and A-375 (ATCC CRL1619TM) cell lines. ..

    other:

    Article Title: Trained Immunity: RoadMap for drug discovery and development
    Article Snippet: Cell line , U-937; Histiocytic Lymphoma , Human , ATCC Product Code: ATCC-CRL-1593.2 , https://www.lgcstandards.com/GB/en/search?text=u937.



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    ATCC u937 cells
    ( A ) Ratiometric rmgCarvi fluorescence of Mtb-rmgCarvi epi (Erdman) cultured in 7H9+OADC or resuspended in DMEM cell culture-based media or immediately upon infection of RAW264.7 macrophages, were measured using a plate reader. cAMP content increases slightly in cell culture media but is significantly increased upon infection (N=4; biological replicates color-coded). Symbols represent means ± SD. One-way ANOVA with Holm-Sidak’s correction ** - p < 0.01, *** - p < 0.001. ( B ) rmgCarvi fluorescence dynamics in Mtb-rmgCarvi epi during the first 24 hours of infection of RAW264.7 macrophages, with or without IFN-ψ activation, or in macrophages where the phagosomal pH was deacidified using 10 mM ammonium chloride (NH 4 Cl), measured by flow cytometry. Intra-mycobacterial cAMP levels drop within 3 hours and is maintained stably over the next 21 hours. cAMP induction upon infection was transient, as indicated by cpGFP and mCherry fluorescence measurements using flow cytometry (N=2-8, 10000 cells per replicate per time point). ( C, D ) Ratiometric rmgCarvi fluorescence values in intracellular Mtb-rmgCarvi epi bacteria upon infection of murine RAW264.7 ( C ) or human <t>U937</t> ( D ) macrophages, captured by fluorescence microscopy and quantified using FIJI (N=3, >300 events per replicate per time point). Kruskal-Wallis test with Dunn’s correction **** - p < 0.0001. ( E ) Mtb-rmgCarvi epi , cultured in 96-well plates, were exposed to a library of ∼240 compounds over 2 days. Correlation plot showing the rmgCarvi fluorescence ratios after 1 h vs 24 h exposure. The dotted black horizontal and vertical lines represent the medians at the corresponding time points. The colored lines indicate + 3 median absolute deviation values. ( F ) Heat-map showing the validation of the activity of some of the chosen compounds from the screen. Compounds 3 and 6 were identified as putative cAMP agonists, similar to GSK286 and Rifampicin (RIF). Compound 9 was included as a negative control and GlutaMAX represents the media control. The time points are indicated on the x-axis , including the fluorescence prior to exposure to compounds (Pre). All data shown are mean of at least three biological replicates. Biological replicates in panels ( C, D ) are color coded.
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    u 937  (ATCC)
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    ATCC u 937
    ( A ) Ratiometric rmgCarvi fluorescence of Mtb-rmgCarvi epi (Erdman) cultured in 7H9+OADC or resuspended in DMEM cell culture-based media or immediately upon infection of RAW264.7 macrophages, were measured using a plate reader. cAMP content increases slightly in cell culture media but is significantly increased upon infection (N=4; biological replicates color-coded). Symbols represent means ± SD. One-way ANOVA with Holm-Sidak’s correction ** - p < 0.01, *** - p < 0.001. ( B ) rmgCarvi fluorescence dynamics in Mtb-rmgCarvi epi during the first 24 hours of infection of RAW264.7 macrophages, with or without IFN-ψ activation, or in macrophages where the phagosomal pH was deacidified using 10 mM ammonium chloride (NH 4 Cl), measured by flow cytometry. Intra-mycobacterial cAMP levels drop within 3 hours and is maintained stably over the next 21 hours. cAMP induction upon infection was transient, as indicated by cpGFP and mCherry fluorescence measurements using flow cytometry (N=2-8, 10000 cells per replicate per time point). ( C, D ) Ratiometric rmgCarvi fluorescence values in intracellular Mtb-rmgCarvi epi bacteria upon infection of murine RAW264.7 ( C ) or human <t>U937</t> ( D ) macrophages, captured by fluorescence microscopy and quantified using FIJI (N=3, >300 events per replicate per time point). Kruskal-Wallis test with Dunn’s correction **** - p < 0.0001. ( E ) Mtb-rmgCarvi epi , cultured in 96-well plates, were exposed to a library of ∼240 compounds over 2 days. Correlation plot showing the rmgCarvi fluorescence ratios after 1 h vs 24 h exposure. The dotted black horizontal and vertical lines represent the medians at the corresponding time points. The colored lines indicate + 3 median absolute deviation values. ( F ) Heat-map showing the validation of the activity of some of the chosen compounds from the screen. Compounds 3 and 6 were identified as putative cAMP agonists, similar to GSK286 and Rifampicin (RIF). Compound 9 was included as a negative control and GlutaMAX represents the media control. The time points are indicated on the x-axis , including the fluorescence prior to exposure to compounds (Pre). All data shown are mean of at least three biological replicates. Biological replicates in panels ( C, D ) are color coded.
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    Image Search Results


    ( A ) Ratiometric rmgCarvi fluorescence of Mtb-rmgCarvi epi (Erdman) cultured in 7H9+OADC or resuspended in DMEM cell culture-based media or immediately upon infection of RAW264.7 macrophages, were measured using a plate reader. cAMP content increases slightly in cell culture media but is significantly increased upon infection (N=4; biological replicates color-coded). Symbols represent means ± SD. One-way ANOVA with Holm-Sidak’s correction ** - p < 0.01, *** - p < 0.001. ( B ) rmgCarvi fluorescence dynamics in Mtb-rmgCarvi epi during the first 24 hours of infection of RAW264.7 macrophages, with or without IFN-ψ activation, or in macrophages where the phagosomal pH was deacidified using 10 mM ammonium chloride (NH 4 Cl), measured by flow cytometry. Intra-mycobacterial cAMP levels drop within 3 hours and is maintained stably over the next 21 hours. cAMP induction upon infection was transient, as indicated by cpGFP and mCherry fluorescence measurements using flow cytometry (N=2-8, 10000 cells per replicate per time point). ( C, D ) Ratiometric rmgCarvi fluorescence values in intracellular Mtb-rmgCarvi epi bacteria upon infection of murine RAW264.7 ( C ) or human U937 ( D ) macrophages, captured by fluorescence microscopy and quantified using FIJI (N=3, >300 events per replicate per time point). Kruskal-Wallis test with Dunn’s correction **** - p < 0.0001. ( E ) Mtb-rmgCarvi epi , cultured in 96-well plates, were exposed to a library of ∼240 compounds over 2 days. Correlation plot showing the rmgCarvi fluorescence ratios after 1 h vs 24 h exposure. The dotted black horizontal and vertical lines represent the medians at the corresponding time points. The colored lines indicate + 3 median absolute deviation values. ( F ) Heat-map showing the validation of the activity of some of the chosen compounds from the screen. Compounds 3 and 6 were identified as putative cAMP agonists, similar to GSK286 and Rifampicin (RIF). Compound 9 was included as a negative control and GlutaMAX represents the media control. The time points are indicated on the x-axis , including the fluorescence prior to exposure to compounds (Pre). All data shown are mean of at least three biological replicates. Biological replicates in panels ( C, D ) are color coded.

    Journal: bioRxiv

    Article Title: A genetically encoded biosensor reveals heterogenous cAMP dynamics coordinating growth and resuscitation in Mycobacterium tuberculosis

    doi: 10.64898/2026.06.02.729633

    Figure Lengend Snippet: ( A ) Ratiometric rmgCarvi fluorescence of Mtb-rmgCarvi epi (Erdman) cultured in 7H9+OADC or resuspended in DMEM cell culture-based media or immediately upon infection of RAW264.7 macrophages, were measured using a plate reader. cAMP content increases slightly in cell culture media but is significantly increased upon infection (N=4; biological replicates color-coded). Symbols represent means ± SD. One-way ANOVA with Holm-Sidak’s correction ** - p < 0.01, *** - p < 0.001. ( B ) rmgCarvi fluorescence dynamics in Mtb-rmgCarvi epi during the first 24 hours of infection of RAW264.7 macrophages, with or without IFN-ψ activation, or in macrophages where the phagosomal pH was deacidified using 10 mM ammonium chloride (NH 4 Cl), measured by flow cytometry. Intra-mycobacterial cAMP levels drop within 3 hours and is maintained stably over the next 21 hours. cAMP induction upon infection was transient, as indicated by cpGFP and mCherry fluorescence measurements using flow cytometry (N=2-8, 10000 cells per replicate per time point). ( C, D ) Ratiometric rmgCarvi fluorescence values in intracellular Mtb-rmgCarvi epi bacteria upon infection of murine RAW264.7 ( C ) or human U937 ( D ) macrophages, captured by fluorescence microscopy and quantified using FIJI (N=3, >300 events per replicate per time point). Kruskal-Wallis test with Dunn’s correction **** - p < 0.0001. ( E ) Mtb-rmgCarvi epi , cultured in 96-well plates, were exposed to a library of ∼240 compounds over 2 days. Correlation plot showing the rmgCarvi fluorescence ratios after 1 h vs 24 h exposure. The dotted black horizontal and vertical lines represent the medians at the corresponding time points. The colored lines indicate + 3 median absolute deviation values. ( F ) Heat-map showing the validation of the activity of some of the chosen compounds from the screen. Compounds 3 and 6 were identified as putative cAMP agonists, similar to GSK286 and Rifampicin (RIF). Compound 9 was included as a negative control and GlutaMAX represents the media control. The time points are indicated on the x-axis , including the fluorescence prior to exposure to compounds (Pre). All data shown are mean of at least three biological replicates. Biological replicates in panels ( C, D ) are color coded.

    Article Snippet: Propagation and infection of RAW264.7 (ATCC: TIB-71) and U937 cells (ATCC: CRL-1593.2) was done as previously described, with some modifications ( ).

    Techniques: Fluorescence, Cell Culture, Infection, Activation Assay, Flow Cytometry, Stable Transfection, Bacteria, Microscopy, Biomarker Discovery, Activity Assay, Negative Control, Control