tyrosine 1248  (R&D Systems)

Journal: Neuron

Article Title: ErbB4 activity in hippocampal-prefrontal synchrony and top-down attention in rodents

doi: 10.1016/j.neuron.2018.03.018

Figure Lengend Snippet: (A) Accuracy rates of indicated mice in 5-CSRTT training (Control (Ctrl), n = 13 mice; ErbB4−/−, n = 13 mice; Two way ANOVA, p<0.001).

Article Snippet: The rabbit anti-phosphorylated-ErbB4 (Tyrosine 1248) antibody was from Cell Signaling (product number 4757S).

Techniques: Control

Journal: Neuron

Article Title: ErbB4 activity in hippocampal-prefrontal synchrony and top-down attention in rodents

doi: 10.1016/j.neuron.2018.03.018

Figure Lengend Snippet: (A, B) Representative in vivo multi-unit raster in ErbB4-CreERT2;ArchT mice in response to yellow LED illumination.

Article Snippet: The rabbit anti-phosphorylated-ErbB4 (Tyrosine 1248) antibody was from Cell Signaling (product number 4757S).

Techniques: In Vivo

Journal: Neuron

Article Title: ErbB4 activity in hippocampal-prefrontal synchrony and top-down attention in rodents

doi: 10.1016/j.neuron.2018.03.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The rabbit anti-phosphorylated-ErbB4 (Tyrosine 1248) antibody was from Cell Signaling (product number 4757S).

Techniques: Recombinant, Software

Journal: Brain

Article Title: Myelin is dependent on the Charcot–Marie–Tooth Type 4H disease culprit protein FRABIN/FGD4 in Schwann cells

doi: 10.1093/brain/aws275

Figure Lengend Snippet: Ablation of Frabin/Fgd4 reduces activation of the RhoGTPase Cdc42 in peripheral nerves in vivo . ( A and B ) Western blot analyses demonstrating that total and active levels of AKT, ErbB2 receptor and JNK, and total levels of MBP and Dlg1, are not changed in sciatic nerve of Fgd4 − / − mice compared with wild-type (wt/wt) mice at the age of 10 weeks. ( C and D ) Active, but not total levels of Cdc42 are significantly reduced in sciatic nerves of adult Fgd4 − / − mice compared with age-matched wild-type mice. Tissues from four wild-type and Fgd4 − / − mice were analysed. *** P < 0.001; Student’s t -test (two-tailed). GADPH = glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The following antibodies were used: AKT-P Serine 473 (Cell Signaling Technology, 1:1000), AKT (Cell Signaling Technology, 1:1000), ErbB2-P Tyrosine 1248 (Abcam, 1:1000), ErbB2 (Abcam, 1:1000), disc large homologue 1 (Dlg1) (BD Transduction Laboratories, 1:1000), myelin basic protein (AbD Serotec, 1:1000), JNK-P Threonine 183/Tyrosine 185 (c-jun-N-terminal kinase) (Cell Signaling, 1:1000), JNK (Cell Signaling, 1:1000), Cdc42 (Abcam, 1:1000 and Cell Signaling, 1:1000), Frabin/Fgd4 (Pineda, 1:1000; peptide used for rabbit immunization: SKGKHSKVSDLISHFE), Frabin/Fgd4 (Transduction Laboratories, 1:500), α-tubulin (Sigma, 1:1000) and glyceraldehyde-3-phosphate dehydrogenase (HyTest, 1:10 000).

Techniques: Activation Assay, In Vivo, Western Blot, Two Tailed Test