Activity Assay:Article Title: Bacterial adenine cross-feeding stems from a purine salvage bottleneck
Article Snippet: .. CGA0092* Type strain; derivative of CGA001 with spontaneous chloramphenicol resistance (2) CGA4004 CGA0092 ΔhupS Δrpa2750 (3) CGA4005 CGA4004 NifA*; a strain with constitutive nitrogenase activity; named Nx in the previous study (3) TIE-1* Environmental isolate (4) DX-1* Environmental isolate (5) 1a1* Environmental isolate (6) 3ClBen#3 Environmental isolate; OR656700 Harwood collection AP1* Environmental isolate (6) NCIB8288* Type strain DSMZ, (6) DSM126* Type strain DSMZ, (6) DSM8283* Type strain DSMZ, (6) RCH550 Environmental isolate; OR656702 Harwood collection RCH500* Environmental isolate (6) ATCC17007* Type strain ATCC, (6) 3ClBen#1 Environmental isolate; OR656699 Harwood collection 0001L* Environmental isolate (6) RCH250 Environmental isolate; OR656697 Harwood collection BIS3* Environmental isolate (6) RCH300 Environmental isolate; OR656698 Harwood collection CEA001* Environmental isolate (6) RSP24* Environmental isolate (6) RCH450 Environmental isolate; OR656701 Harwood collection RCH400 Environmental isolate; OR659638 Harwood collection RCH350* Environmental isolate (6) KD1* Environmental isolate (6) DCP3* Environmental isolate (6) RCH150 Environmental isolate; OR656709 Harwood collection RCH200 Environmental isolate; OR656708 Harwood collection ATCCBAA37* Type strain ATCC WS17* Environmental isolate (7) P4* Environmental isolate (8) Strain#7 Environmental isolate; OR656710 Harwood collection DSM7375* Type strain DSMZ DSM131* Type strain DSMZ No.7* Environmental isolate (9) RSP22 Environmental isolate; OR656707 Harwood collection RSP21 Environmental isolate; OR656706 Harwood collection S55* Environmental isolate (9) ATCC17001* Type strain ATCC RSP15 Environmental isolate; OR656705 Harwood collection RSP10 Environmental isolate; OR656704 Harwood collection CGD052 Environmental isolate; OR656703 Harwood collection RCH50 Environmental isolate; OR656696 Harwood collection BIS6 Environmental isolate; OR656711 (10) 42OL* Environmental isolate (11) RSP20 Environmental isolate; OR656713 Harwood collection BIS10 Environmental isolate; OR656712 (10) ATCC17009 Type strain; OR656714 ATCC BisA53* Environmental isolate (12) BisB5* Environmental isolate (12) HaA2* Environmental isolate (12) .. MG1655 Wild-type K-12 (13) MG1655 ΔpurH::KmR ΔpurH::KmR; mutant allele came from Keio strain JW3970; Referred to as E. coli ΔpurH in the paper. this study MG1655 ΔpyrC::KmR ΔpyrC::KmR; mutant allele came from Keio strain JW1049; Referred to as E. coli ΔpyrC in the paper. this study NEB10β DH10B derivative; Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMSmcrBC) New England Biolabs JW3970 ΔpurH:: KmR; Keio knockout strain (14) JW1049 ΔpyrC:: KmR; Keio knockout strain (14) * 16S rRNA gene sequence is obtained from the Integrated Microbial Genome (IMG) database (https://img.jgi.doe.gov/).
Article Title: A purine salvage bottleneck leads to bacterial adenine cross-feeding
Article Snippet: .. CGA0092* Type strain; derivative of CGA001 with spontaneous chloramphenicol resistance (1) CGA4004 CGA0092 ΔhupS Δrpa2750 (2) CGA4005 CGA4004 NifA*; a strain with constitutive nitrogenase activity; named Nx in the previous study (2) TIE-1* Environmental isolate (3) DX-1* Environmental isolate (4) 1a1* Environmental isolate (5) 3ClBen#3 Environmental isolate; OR656700 Harwood collection AP1* Environmental isolate (5) NCIB8288* Type strain DSMZ, (5) DSM126* Type strain DSMZ, (5) DSM8283* Type strain DSMZ, (5) RCH550 Environmental isolate; OR656702 Harwood collection RCH500* Environmental isolate (5) ATCC17007* Type strain ATCC, (5) 3ClBen#1 Environmental isolate; OR656699 Harwood collection 0001L* Environmental isolate (5) RCH250 Environmental isolate; OR656697 Harwood collection BIS3* Environmental isolate (5) RCH300 Environmental isolate; OR656698 Harwood collection CEA001* Environmental isolate (5) RSP24* Environmental isolate (5) RCH450 Environmental isolate; OR656701 Harwood collection RCH400 Environmental isolate; OR659638 Harwood collection RCH350* Environmental isolate (5) KD1* Environmental isolate (5) DCP3* Environmental isolate (5) RCH150 Environmental isolate; OR656709 Harwood collection RCH200 Environmental isolate; OR656708 Harwood collection ATCCBAA37* Type strain ATCC WS17* Environmental isolate (6) P4* Environmental isolate (7) Strain#7 Environmental isolate; OR656710 Harwood collection DSM7375* Type strain DSMZ DSM131* Type strain DSMZ No.7* Environmental isolate (8) RSP22 Environmental isolate; OR656707 Harwood collection RSP21 Environmental isolate; OR656706 Harwood collection S55* Environmental isolate (8) ATCC17001* Type strain ATCC RSP15 Environmental isolate; OR656705 Harwood collection RSP10 Environmental isolate; OR656704 Harwood collection CGD052 Environmental isolate; OR656703 Harwood collection RCH50 Environmental isolate; OR656696 Harwood collection BIS6 Environmental isolate; OR656711 (9) 42OL* Environmental isolate (10) RSP20 Environmental isolate; OR656713 Harwood collection BIS10 Environmental isolate; OR656712 (9) ATCC17009 Type strain; OR656714 ATCC BisA53* Environmental isolate (11) BisB5* Environmental isolate (11) HaA2* Environmental isolate (11) .. MG1655 Wild-type K-12 (12) MG1655 ΔpurH::KmR ΔpurH::KmR; mutant allele came from Keio strain JW3970; Referred to as E. coli ΔpurH in the paper. this study MG1655 ΔpyrC::KmR ΔpyrC::KmR; mutant allele came from Keio strain JW1049; Referred to as E. coli ΔpyrC in the paper. this study NEB10β DH10B derivative; Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrrhsdRMS-mcrBC) New England Biolabs JW3970 ΔpurH:: KmR; Keio knockout strain (13) JW1049 ΔpyrC:: KmR; Keio knockout strain (13) * 16S rRNA gene sequence is obtained from the Integrated Microbial Genome (IMG) database 94 (https://img.jgi.doe.gov/).
Derivative Assay:Article Title: Engineered immunostimulatory bacterial strains and uses thereof
Article Snippet: .. Exemplary strains include Salmonella typhimurium strains derived from strains designated as AST100, VNP20009, or strains YS1646 (ATCC #202165), RE88, SL7207, χ8429, χ8431, χ8468, and the wild-type strain ATCC #14028. ..
Control:Article Title: Genotypic and phenotypic characterisation of a nosocomial outbreak of Candida auris in Spain during 5 years.
Article Snippet: phenotypic characterisation Antifungal susceptibility testing (AFST) was carried out with Sensititre® YeastOne YO10 (Thermo Fisher Scientific, USA). .. Tentative breakpoints from CDC were used to interpret the susceptibility of the isolates.10 Isolates were then classified as aggregating or non- aggregating, as described by Szekely et al.11 In all cases, type strain ATCC® MYA- 5001® was used as a control. .. sequencing Several colonies of each isolate were introduced into Eppendorf tubes with distilled water and centrifuged at 5000 rpm for 5 min.
Modification:Article Title: Engineered immunostimulatory bacterial strains and uses thereof
Article Snippet: No. 6,863,894) and YS1646 and 41.2.9 are used interchangeably and each refer to the strain deposited with the American Type Culture Collection (ATCC) and assigned Accession No. 202165. .. VNP20009 is a modified attenuated strain of Salmonella typhimurium, which contains deletions in msbB and purI, and was generated from wild type strain ATCC #14028. .. As used herein, the strain designations YS1456 and 8.7 are used interchangeably and each refer to the strain deposited with the American Type Culture Collection and assigned Accession No. 202164 (see, U.S. Pat.
Generated:Article Title: Engineered immunostimulatory bacterial strains and uses thereof
Article Snippet: No. 6,863,894) and YS1646 and 41.2.9 are used interchangeably and each refer to the strain deposited with the American Type Culture Collection (ATCC) and assigned Accession No. 202165. .. VNP20009 is a modified attenuated strain of Salmonella typhimurium, which contains deletions in msbB and purI, and was generated from wild type strain ATCC #14028. .. As used herein, the strain designations YS1456 and 8.7 are used interchangeably and each refer to the strain deposited with the American Type Culture Collection and assigned Accession No. 202164 (see, U.S. Pat.
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