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Keygen Biotech tunel staining kit
Tunel Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tunel+staining+kit/staining+tunel/pm42308359-134-8-11
Average 86 stars, based on 1 article reviews
tunel staining kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Multiplex Assay:

Article Title: Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer.
Article Snippet: .. Multiplex Immunohistochemical (mIHC): Slides of the ovarian cancer tissue chip were incubated with anti-EPCAM (ab223582, Abcam, 1:1000, RRID:AB_2762366), anti-CD44 (#37259, Cell Signaling Technology, 1:500, RRID:AB_2750879), anti-CD68 (ab213363, Abcam, 1:1000, AB_2801637), anti-OPN (ab214050, Abcam, 1:1000, AB_2894860), and anti-ODC1 (ab270268, Abcam, 1:500) antibodies using the Think color-6 color mIHC kit (Freethinking, FH36100R) according to the manufacturer’s instructions, followed by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining using the TUNEL staining kit (KGA1401-20, Keygen Biotech) according to the manufacturer’s instructions. ..

Immunohistochemical staining:

Article Title: Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer.
Article Snippet: .. Multiplex Immunohistochemical (mIHC): Slides of the ovarian cancer tissue chip were incubated with anti-EPCAM (ab223582, Abcam, 1:1000, RRID:AB_2762366), anti-CD44 (#37259, Cell Signaling Technology, 1:500, RRID:AB_2750879), anti-CD68 (ab213363, Abcam, 1:1000, AB_2801637), anti-OPN (ab214050, Abcam, 1:1000, AB_2894860), and anti-ODC1 (ab270268, Abcam, 1:500) antibodies using the Think color-6 color mIHC kit (Freethinking, FH36100R) according to the manufacturer’s instructions, followed by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining using the TUNEL staining kit (KGA1401-20, Keygen Biotech) according to the manufacturer’s instructions. ..

Incubation:

Article Title: Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer.
Article Snippet: .. Multiplex Immunohistochemical (mIHC): Slides of the ovarian cancer tissue chip were incubated with anti-EPCAM (ab223582, Abcam, 1:1000, RRID:AB_2762366), anti-CD44 (#37259, Cell Signaling Technology, 1:500, RRID:AB_2750879), anti-CD68 (ab213363, Abcam, 1:1000, AB_2801637), anti-OPN (ab214050, Abcam, 1:1000, AB_2894860), and anti-ODC1 (ab270268, Abcam, 1:500) antibodies using the Think color-6 color mIHC kit (Freethinking, FH36100R) according to the manufacturer’s instructions, followed by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining using the TUNEL staining kit (KGA1401-20, Keygen Biotech) according to the manufacturer’s instructions. ..

Labeling:

Article Title: Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer.
Article Snippet: .. Multiplex Immunohistochemical (mIHC): Slides of the ovarian cancer tissue chip were incubated with anti-EPCAM (ab223582, Abcam, 1:1000, RRID:AB_2762366), anti-CD44 (#37259, Cell Signaling Technology, 1:500, RRID:AB_2750879), anti-CD68 (ab213363, Abcam, 1:1000, AB_2801637), anti-OPN (ab214050, Abcam, 1:1000, AB_2894860), and anti-ODC1 (ab270268, Abcam, 1:500) antibodies using the Think color-6 color mIHC kit (Freethinking, FH36100R) according to the manufacturer’s instructions, followed by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining using the TUNEL staining kit (KGA1401-20, Keygen Biotech) according to the manufacturer’s instructions. ..

TUNEL Assay:

Article Title: Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer.
Article Snippet: .. Multiplex Immunohistochemical (mIHC): Slides of the ovarian cancer tissue chip were incubated with anti-EPCAM (ab223582, Abcam, 1:1000, RRID:AB_2762366), anti-CD44 (#37259, Cell Signaling Technology, 1:500, RRID:AB_2750879), anti-CD68 (ab213363, Abcam, 1:1000, AB_2801637), anti-OPN (ab214050, Abcam, 1:1000, AB_2894860), and anti-ODC1 (ab270268, Abcam, 1:500) antibodies using the Think color-6 color mIHC kit (Freethinking, FH36100R) according to the manufacturer’s instructions, followed by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining using the TUNEL staining kit (KGA1401-20, Keygen Biotech) according to the manufacturer’s instructions. ..

Article Title: Inhibition of NLRP3 Inflammasome Alleviates Postoperative Cognitive Impairment by Suppressing the HMGB-1/TLR4/NF-κB Pathway.
Article Snippet: Perioperative neurocognitive disorders (PNDs), main complications of surgery and anesthesia in the elderly, are mainly associated with neuroinflammation and remain poorly understood.. This study aimed to explore the function of the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome in the development of PNDs.. Male C57BL/6 mice underwent right carotid artery exposure surgery, with or without treatment with adeno-associated virus vectors carrying NLRP3 (AAV-NLRP3), which was administered either intracerebroventricularly or via tail vein injection.

Article Title: mmu_circ_0012122/mmu-miR-1843-5p/Sertad2 axis: A novel regulatory pathway in rabies virus infection.
Article Snippet: For TUNEL staining, N2A cells were cultured on LabTek Chamber Slides and subjected to the corresponding transfection and RABV infection treatments. .. After treatment, N2A cells were stained using the TUNEL staining kit (KeyGEN BioTECH, Cat.No. ..

Article Title: Effects of Different Exercise Intensities on the Rat Model of Heart Failure.
Article Snippet: The results of HE and Masson staining were observed under a microscope (Nikon, Tokyo, Japan). .. TUNEL staining: Apoptosis was performed using the TUNEL staining kit (KeyGEN, Jiangsu, China). ..

Article Title: Arbutin alleviates intestinal colitis by regulating neutrophil extracellular traps formation and microbiota composition.
Article Snippet: Background: Ulcerative colitis (UC) is a chronic recurrent intestinal disease lacking effective treatments. β-arbutin, a glycoside extracted from the Arctostaphylos uva-ursi leaves, that can regulate many pathological processes.. However, the effects of β-arbutin on UC remain unknown.. Purpose: In this study, we investigated the role of β-arbutin in relieving colitis and explored its potential mechanisms in a mouse model of dextran sulfate sodium (DSS)–induced colitis.

Article Title: Gut microbial metabolite alleviates osteoporosis by attenuating AKT-NFATc1 signaling pathway and ROS production.
Article Snippet: Postmenopausal osteoporosis is a prevalent bone disorder characterized by an imbalance in bone homeostasis following estrogen decline, which leads to progressive bone loss.. Research has shown that estrogen deprivationinduced osteoporosis (OVX) is partly dependent on the gut microbiota and its metabolites, a process that can be therapeutically targeted through supplementation with probiotics or the metabolites themselves.. Multiple studies have revealed the multifaceted roles of indole and its derivatives in bone health and the pathophysiology of skeletal disorders.

Article Title: MRI-Based Multifunctional Nanoliposomes for Enhanced HCC Therapy and Diagnosis.
Article Snippet: The morbidity and mortality rates of hepatocellular carcinoma (HCC) are high and continue to increase.. The antitumor effects of single therapies are limited because of tumor heterogeneity and drug resistance, and the lack of real-time monitoring of tumor progression during the treatment process leads to poor therapeutic outcomes.. Therefore, novel nanodelivery platforms combining tumor therapy and diagnosis have garnered extensive attention.

Article Title: CXCR1 and CXCR2 Antagonism with G31P Attenuates Chemotherapy-Induced Lung Inflammation and Augments the Gefitinib Therapeutic Response in Lung Cancer
Article Snippet: .. Apoptotic cells in tumor tissue sections were assessed using TUNEL staining kit (RFP-TUNEL Apoptosis detection Kit, Cat# KGA710, KeyGEN Biotechnology, Nanjing, China). ..

Staining:

Article Title: Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer.
Article Snippet: .. Multiplex Immunohistochemical (mIHC): Slides of the ovarian cancer tissue chip were incubated with anti-EPCAM (ab223582, Abcam, 1:1000, RRID:AB_2762366), anti-CD44 (#37259, Cell Signaling Technology, 1:500, RRID:AB_2750879), anti-CD68 (ab213363, Abcam, 1:1000, AB_2801637), anti-OPN (ab214050, Abcam, 1:1000, AB_2894860), and anti-ODC1 (ab270268, Abcam, 1:500) antibodies using the Think color-6 color mIHC kit (Freethinking, FH36100R) according to the manufacturer’s instructions, followed by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining using the TUNEL staining kit (KGA1401-20, Keygen Biotech) according to the manufacturer’s instructions. ..

Article Title: Inhibition of NLRP3 Inflammasome Alleviates Postoperative Cognitive Impairment by Suppressing the HMGB-1/TLR4/NF-κB Pathway.
Article Snippet: Perioperative neurocognitive disorders (PNDs), main complications of surgery and anesthesia in the elderly, are mainly associated with neuroinflammation and remain poorly understood.. This study aimed to explore the function of the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome in the development of PNDs.. Male C57BL/6 mice underwent right carotid artery exposure surgery, with or without treatment with adeno-associated virus vectors carrying NLRP3 (AAV-NLRP3), which was administered either intracerebroventricularly or via tail vein injection.

Article Title: mmu_circ_0012122/mmu-miR-1843-5p/Sertad2 axis: A novel regulatory pathway in rabies virus infection.
Article Snippet: For TUNEL staining, N2A cells were cultured on LabTek Chamber Slides and subjected to the corresponding transfection and RABV infection treatments. .. After treatment, N2A cells were stained using the TUNEL staining kit (KeyGEN BioTECH, Cat.No. ..

Article Title: Effects of Different Exercise Intensities on the Rat Model of Heart Failure.
Article Snippet: The results of HE and Masson staining were observed under a microscope (Nikon, Tokyo, Japan). .. TUNEL staining: Apoptosis was performed using the TUNEL staining kit (KeyGEN, Jiangsu, China). ..

Article Title: Arbutin alleviates intestinal colitis by regulating neutrophil extracellular traps formation and microbiota composition.
Article Snippet: Background: Ulcerative colitis (UC) is a chronic recurrent intestinal disease lacking effective treatments. β-arbutin, a glycoside extracted from the Arctostaphylos uva-ursi leaves, that can regulate many pathological processes.. However, the effects of β-arbutin on UC remain unknown.. Purpose: In this study, we investigated the role of β-arbutin in relieving colitis and explored its potential mechanisms in a mouse model of dextran sulfate sodium (DSS)–induced colitis.

Article Title: Gut microbial metabolite alleviates osteoporosis by attenuating AKT-NFATc1 signaling pathway and ROS production.
Article Snippet: Postmenopausal osteoporosis is a prevalent bone disorder characterized by an imbalance in bone homeostasis following estrogen decline, which leads to progressive bone loss.. Research has shown that estrogen deprivationinduced osteoporosis (OVX) is partly dependent on the gut microbiota and its metabolites, a process that can be therapeutically targeted through supplementation with probiotics or the metabolites themselves.. Multiple studies have revealed the multifaceted roles of indole and its derivatives in bone health and the pathophysiology of skeletal disorders.

Article Title: MRI-Based Multifunctional Nanoliposomes for Enhanced HCC Therapy and Diagnosis.
Article Snippet: The morbidity and mortality rates of hepatocellular carcinoma (HCC) are high and continue to increase.. The antitumor effects of single therapies are limited because of tumor heterogeneity and drug resistance, and the lack of real-time monitoring of tumor progression during the treatment process leads to poor therapeutic outcomes.. Therefore, novel nanodelivery platforms combining tumor therapy and diagnosis have garnered extensive attention.

Article Title: CXCR1 and CXCR2 Antagonism with G31P Attenuates Chemotherapy-Induced Lung Inflammation and Augments the Gefitinib Therapeutic Response in Lung Cancer
Article Snippet: .. Apoptotic cells in tumor tissue sections were assessed using TUNEL staining kit (RFP-TUNEL Apoptosis detection Kit, Cat# KGA710, KeyGEN Biotechnology, Nanjing, China). ..



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Image Search Results


Co-administration of C. chinensis and B. breve S1 improved the pathological manifestations of AD mice. (A) Construction of B. breve S1 AD mice (* P < 0.05 ** P < 0.01 *** P < 0.001 vs D1, # P < 0.05 ## P < 0.01 ### P < 0.001 vs D7). (B) Statistical chart of latency time of Barnes maze test in AD mice ( n = 5). (C) Statistical chart of target quadrant ratio of Barnes maze test in mice ( n = 5). (D) Statistical chart of total displacement of Barnes maze test in mice ( n = 5). (E) The content of A β in serum ( n = 5). (F) The content of A β in brain ( n = 5). n. s., no significant difference, # P < 0.05 ### P < 0.001 vs Control group, * P < 0.05 ** P < 0.01 *** P < 0.001 vs Model-free group, n.s. not significant. (G) Results of Congo red staining (arrow: amyloid protein deposition, n = 5, scale bar: 500 μm, × 40). (H) Results of HE staining ( n = 5, scale bar: 50 μm, × 400). (I) Results of TUNEL staining (arrow: apoptotic cells, n = 5, scale bar: 50 μm, × 400).

Journal: Chinese Herbal Medicines

Article Title: Isoquinoline alkaloids in Coptis chinensis to treat Alzheimer’s disease through promoting growth of Bifidobacterium breve inhibiting abnormal autophagy using a novel AI high-content intelligent imaging system

doi: 10.1016/j.chmed.2026.02.003

Figure Lengend Snippet: Co-administration of C. chinensis and B. breve S1 improved the pathological manifestations of AD mice. (A) Construction of B. breve S1 AD mice (* P < 0.05 ** P < 0.01 *** P < 0.001 vs D1, # P < 0.05 ## P < 0.01 ### P < 0.001 vs D7). (B) Statistical chart of latency time of Barnes maze test in AD mice ( n = 5). (C) Statistical chart of target quadrant ratio of Barnes maze test in mice ( n = 5). (D) Statistical chart of total displacement of Barnes maze test in mice ( n = 5). (E) The content of A β in serum ( n = 5). (F) The content of A β in brain ( n = 5). n. s., no significant difference, # P < 0.05 ### P < 0.001 vs Control group, * P < 0.05 ** P < 0.01 *** P < 0.001 vs Model-free group, n.s. not significant. (G) Results of Congo red staining (arrow: amyloid protein deposition, n = 5, scale bar: 500 μm, × 40). (H) Results of HE staining ( n = 5, scale bar: 50 μm, × 400). (I) Results of TUNEL staining (arrow: apoptotic cells, n = 5, scale bar: 50 μm, × 400).

Article Snippet: TUNEL staining kit (lot number: 01981015) was purchased from Shanghai Epizyme Biomedical Technology Co., Ltd. (Shanghai, China).

Techniques: Control, Staining, TUNEL Assay

OM-MSCs-Exo alleviated cognitive impairment and neuroinflammation in AD mice through FGFR1. A Swimming distance, swimming time, number of platform arrivals, and latency to first entry ( n = 6). B The hippocampal tissues of mice were stained with HE. C Nissl staining was performed in the hippocampus of mice. D TUNEL assay. E Data plot of the TUNEL assay. F Levels of IL-1β, TNF-α, and IL-6 in mice hippocampus. G WB analysis of Aβ, p-Tau/Tau in mice hippocampus. H Aβ 1–42 levels were detected. I FGFR1 and PLCγ1 levels were measured. J Levels of p-NF-κB/NF-κB. K , L IF staining of CD86 and CD206 in mice hippocampus. M Levels of microglia M1 and M2 polarization–related factors in mice hippocampus ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001. Normality was confirmed using the Shapiro–Wilk test. Thereafter, data were analyzed with a one-way ANOVA (followed by Tukey’s post hoc test) for multiple-group comparisons

Journal: Molecular Neurobiology

Article Title: Olfactory Mucosa Mesenchymal Stem Cell–Derived Exosomes Enhance Microglia M2 Polarization via the FGFR1/PLCγ1 Axis to Alleviate Alzheimer’s Disease

doi: 10.1007/s12035-026-05797-w

Figure Lengend Snippet: OM-MSCs-Exo alleviated cognitive impairment and neuroinflammation in AD mice through FGFR1. A Swimming distance, swimming time, number of platform arrivals, and latency to first entry ( n = 6). B The hippocampal tissues of mice were stained with HE. C Nissl staining was performed in the hippocampus of mice. D TUNEL assay. E Data plot of the TUNEL assay. F Levels of IL-1β, TNF-α, and IL-6 in mice hippocampus. G WB analysis of Aβ, p-Tau/Tau in mice hippocampus. H Aβ 1–42 levels were detected. I FGFR1 and PLCγ1 levels were measured. J Levels of p-NF-κB/NF-κB. K , L IF staining of CD86 and CD206 in mice hippocampus. M Levels of microglia M1 and M2 polarization–related factors in mice hippocampus ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001. Normality was confirmed using the Shapiro–Wilk test. Thereafter, data were analyzed with a one-way ANOVA (followed by Tukey’s post hoc test) for multiple-group comparisons

Article Snippet: According to the manufacturer’s instructions, sections were stained with a TdT-mediated dUTP nick-end labeling (TUNEL) staining kit (40306ES50; YEASEN) and a DAPI working solution under dark conditions.

Techniques: Staining, TUNEL Assay