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autofluorescence  (Vector Laboratories)


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    Structured Review

    Vector Laboratories autofluorescence
    Autofluorescence, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trueview+autofluorescence/Vector%C2%AE+TrueVIEW%C2%AE+Autofluorescence+Quenching+Kit/pmc12919959-231-1-4
    Average 99 stars, based on 915 article reviews
    autofluorescence - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: A hierarchical lectin-based multimodal workflow for spatial mapping of extracellular matrix glycans in fibrotic hearts.
    Article Snippet: After washing with PBS, the sections were further incubated with streptavidin-conjugated Alexa Fluor 647 (1 μg/mL in PBS; Thermo Fisher Scientific, Waltham, MA), a membrane marker wheat germ agglutinin (WGA; fluorescein isothiocyanate [FITC]–conjugated, 2 μg/mL in PBS; Vector Laboratories), and Hoechst 33342 (0.5 μg/mL in PBS; Dojindo, Kumamoto, Japan), for 1 h at RT. .. The autofluorescence in the sections was then quenched using a TrueVIEW autofluorescence quenching kit (Vector Laboratories), mounted using ProLong Diamond antifade reagent (Thermo Fisher Scientific), and scanned using a digital slide scanner (NanoZoomer S60; Hamamatsu Photonics, Hamamatsu, Japan) or a fluorescence microscope (BZ-X710; Keyence, Osaka, Japan). .. The area quantification was performed using measurement software (Hybrid Cell Count and Macro Cell Count; Keyence).

    Article Title: Macrophage NEDD4L restrains liver fibrosis by preventing scar-associated macrophage expansion via ubiquitination of phospho-SMAD3.
    Article Snippet: Next, primary antibodies were incubated overnight at 4°C, and secondary antibodies (Alexa Fluor 488 or 555 goat anti-rabbit IgG [H+L], Cell Signaling Technology, catalog 4412S or 4413S; Alexa Fluor 488 or 555 goat anti-mouse IgG [H+L], Cell Signaling Technology, catalog 4408S or 4409S, Danvers, MA, USA) were incubated for 1 hour at room temperature. .. TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, catalog ZK0818, CA, USA) was used to eliminate non-specific fluorescence. .. Nuclear staining was performed using 4’, 6’-diamino-2-phenylindole (DAPI) (Beyotime, catalog P0131, Shanghai, China).

    Microscopy:

    Article Title: A hierarchical lectin-based multimodal workflow for spatial mapping of extracellular matrix glycans in fibrotic hearts.
    Article Snippet: After washing with PBS, the sections were further incubated with streptavidin-conjugated Alexa Fluor 647 (1 μg/mL in PBS; Thermo Fisher Scientific, Waltham, MA), a membrane marker wheat germ agglutinin (WGA; fluorescein isothiocyanate [FITC]–conjugated, 2 μg/mL in PBS; Vector Laboratories), and Hoechst 33342 (0.5 μg/mL in PBS; Dojindo, Kumamoto, Japan), for 1 h at RT. .. The autofluorescence in the sections was then quenched using a TrueVIEW autofluorescence quenching kit (Vector Laboratories), mounted using ProLong Diamond antifade reagent (Thermo Fisher Scientific), and scanned using a digital slide scanner (NanoZoomer S60; Hamamatsu Photonics, Hamamatsu, Japan) or a fluorescence microscope (BZ-X710; Keyence, Osaka, Japan). .. The area quantification was performed using measurement software (Hybrid Cell Count and Macro Cell Count; Keyence).

    Incubation:

    Article Title: HMGB1-Induced Perturbations in the Uterine Immune Microenvironment and Implantation Failure in Rats.
    Article Snippet: .. Following two washes with PBS, sections were incubated with DAPI (4′, 6- 214 diamidino-2-phenylindoledihydrochloride, Roche) for 45 min. Tissue autofluorescence was 215 quenched using TrueVIEW Autofluorescence Quenching Kit (SP-8400-15, Vector Labs) and 216 the slides were mounted using Vectashield Vibrance Antifade Mounting Medium (catalogue 217 number H-1700, Vector Labs). .. Slides stained were scanned using the Fluoview 3000 Olympus 218 confocal microscope at 60X and 120X magnifications.



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    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Image Search Results


    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Journal: Journal of Translational Autoimmunity

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

    doi: 10.1016/j.jtauto.2025.100345

    Figure Lengend Snippet: Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Article Snippet: Following application of TrueView autofluorescence quencher, slides were mounted using Vectashield Vibrance Antifade mounting media with DAPI (SP-8500-15; Vector Laboratories; USA) and air dried for 2 h before imaging on the Nikon Ti2-E microscope.

    Techniques: Fluorescence