autofluorescence (Vector Laboratories)
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Autofluorescence, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trueview+autofluorescence/Vector%C2%AE+TrueVIEW%C2%AE+Autofluorescence+Quenching+Kit/pmc12919959-231-1-4
Average 99 stars, based on 915 article reviews
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Fluorescence:Article Title: A hierarchical lectin-based multimodal workflow for spatial mapping of extracellular matrix glycans in fibrotic hearts. Article Snippet: After washing with PBS, the sections were further incubated with streptavidin-conjugated Alexa Fluor 647 (1 μg/mL in PBS; Thermo Fisher Scientific, Waltham, MA), a membrane marker wheat germ agglutinin (WGA; fluorescein isothiocyanate [FITC]–conjugated, 2 μg/mL in PBS; Vector Laboratories), and Hoechst 33342 (0.5 μg/mL in PBS; Dojindo, Kumamoto, Japan), for 1 h at RT. .. The Article Title: Macrophage NEDD4L restrains liver fibrosis by preventing scar-associated macrophage expansion via ubiquitination of phospho-SMAD3. Article Snippet: Next, primary antibodies were incubated overnight at 4°C, and secondary antibodies (Alexa Fluor 488 or 555 goat anti-rabbit IgG [H+L], Cell Signaling Technology, catalog 4412S or 4413S; Alexa Fluor 488 or 555 goat anti-mouse IgG [H+L], Cell Signaling Technology, catalog 4408S or 4409S, Danvers, MA, USA) were incubated for 1 hour at room temperature. .. Microscopy:Article Title: A hierarchical lectin-based multimodal workflow for spatial mapping of extracellular matrix glycans in fibrotic hearts. Article Snippet: After washing with PBS, the sections were further incubated with streptavidin-conjugated Alexa Fluor 647 (1 μg/mL in PBS; Thermo Fisher Scientific, Waltham, MA), a membrane marker wheat germ agglutinin (WGA; fluorescein isothiocyanate [FITC]–conjugated, 2 μg/mL in PBS; Vector Laboratories), and Hoechst 33342 (0.5 μg/mL in PBS; Dojindo, Kumamoto, Japan), for 1 h at RT. .. The Incubation:Article Title: HMGB1-Induced Perturbations in the Uterine Immune Microenvironment and Implantation Failure in Rats. Article Snippet: .. Following two washes with PBS, sections were incubated with DAPI (4′, 6- 214 diamidino-2-phenylindoledihydrochloride, Roche) for 45 min. Tissue autofluorescence was 215 quenched using |
![Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0342/pmc12810342/pmc12810342__gr3.jpg)