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tris based washes  (Proteintech)


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    Proteintech tris based washes
    Tris Based Washes, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tris+based+washes/PRDM10+Antibody/pm41188020-429-1-18
    Average 96 stars, based on 1716 article reviews
    tris based washes - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: Aberrant NSUN1 Activity Connects m5C RNA Modification to TDP-43 Neurotoxicity in ALS/FTD
    Article Snippet: All Tris based washes were 5 min. Primary antibodies used were rabbit anti-phosphorylated (pS409/410) TDP-43 (1 in 5,000, Proteintech #80007-I-RR) and rabbit anti-NSUN1 (1:5,000; Proteintech #10448-1-AP).

    Article Title: Aberrant NSUN1 activity connects m 5 C-RNA modification to TDP-43 neurotoxicity in ALS/FTD
    Article Snippet: Slides were counterstained with Harris hematoxylin (30 s), washed in running tap water (10 min) dehydrated, cleared in xylene and mounted in cytoseal XYL (#8312–4; Thermo Fisher Scientific).

    Article Title: Aberrant NSUN1 activity connects m 5 C-RNA modification to TDP-43 neurotoxicity in ALS/FTD.
    Article Snippet: Slides were washed in 0.1 M Tris pH 7.6 then 0.1 M Tris pH 7.6 with 2% FBS and incubated with an avidin-conjugated horseradish peroxidase (#PK-6100; Vectastain ABC kit) made up in Tris pH 7.6 with 2% FBS (1 h at RT).



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    Key resources table

    Journal: iScience

    Article Title: Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain

    doi: 10.1016/j.isci.2023.106350

    Figure Lengend Snippet: Key resources table

    Article Snippet: The following day, the neurons were washed for 3 × 5 min washes in 1 mL of Duolink Wash Buffer A (0.01 M Tris-Base, 0.15 M NaCl, 0.05% Tween 20, pH 7.4) followed by incubation in 40 μL of Duolink PLA MINUS (Sigma Aldrich, DUO82004) and PLUS probes (Sigma Aldrich, DUO82002) at 37°C for 1 h. Duolink PLA probes were diluted in antibody diluent (Sigma Aldrich, DUO82008) at a 1:5 dilution.

    Techniques: Recombinant, In Situ, Knock-In, Software, Imaging