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Journal: BMC cancer
Article Title: Combined inhibition of ribonucleotide reductase and WEE1 induces synergistic anticancer activity in Ewing's sarcoma cells.
doi: 10.1186/s12885-025-13691-2
Figure Lengend Snippet: Fig. 1 RNRi and WEE1i cooperate in inducing cell death in ES cells. Cells were exposed to triapine in combination with (A) adavosertib or (B) ZN-c3 for 48 h. Cell death was determined by flow-cytometric analysis of PI uptake. Means ± SEM of each three independent measurements are shown
Article Snippet: Twenty-four hours after seeding, cells were treated with
Techniques:
Journal: BMC cancer
Article Title: Combined inhibition of ribonucleotide reductase and WEE1 induces synergistic anticancer activity in Ewing's sarcoma cells.
doi: 10.1186/s12885-025-13691-2
Figure Lengend Snippet: Fig. 2 CI values for triapine plus adavosertib or ZN-c3 in ES cells. Based on data from (A) Fig. 1A and (B) Fig. 1B, CI values were calculated with the Chou- Talalay method
Article Snippet: Twenty-four hours after seeding, cells were treated with
Techniques:
Journal: BMC cancer
Article Title: Combined inhibition of ribonucleotide reductase and WEE1 induces synergistic anticancer activity in Ewing's sarcoma cells.
doi: 10.1186/s12885-025-13691-2
Figure Lengend Snippet: Fig. 6 RNRi and WEE1i cooperate in inducing p53 target gene expression. Cells were exposed to triapine in combination with adavosertib or ZN-c3 for 24 h. CDKN1A and BBC3 expression levels were determined by real-time RT-PCR and normalised to B2M expression levels; relative gene expression levels are the ratio of treated cells to untreated cells. Means ± SEM of each three independent measurements are shown (*p < 0.05, **p < 0.01, ***p < 0.001)
Article Snippet: Twenty-four hours after seeding, cells were treated with
Techniques: Targeted Gene Expression, Expressing, Quantitative RT-PCR, Gene Expression