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trap staining solution  (Servicebio Inc)

 
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    Servicebio Inc trap staining solution
    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pmc12670961-217-11-15
    Average 86 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1"

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.10.040

    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Figure Legend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Techniques Used: Immunohistochemical staining, Staining, Immunofluorescence

    Related Articles

    Staining:

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
    Article Snippet: .. Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light. ..

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice.
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression.
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 houruntil a reddish-purple precipitate indicative of TRAPactivity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: The role of Lactobacillus rhamnosus in improving estrogen deficiency-induced osteoporosis by inhibiting NLRP3 inflammasome activation.
    Article Snippet: .. For tartrate-resistant acid phosphatase (TRAP) staining, deparaffinized and rehydrated sections were incubated with TRAP staining solution (Cat# G1050, Servicebio, China) at 37 ◦C in the dark for 50 min. After staining, sections were rinsed with distilled water and briefly counterstained with hematoxylin for 5 min, followed by the same dehydration, clearing, and mounting procedures described above. ..

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition
    Article Snippet: .. Based on our previous research [ ], RAW 264.7 cells were incubated with 100 μg/mL BTO and pBTO, and stimulated with 50 ng/mL RANKL (peprotech, USA) for 3–5 days and then subjected to TRAP staining (Servicebio, China). .. The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Article Title: Ultrasound activated Zn Ga layered double oxide as ROS antimicrobial-healing system boosts antimicrobial effect and infected bone regeneration
    Article Snippet: Bone infections are destructive diseases caused by microbial seeding, and sonodynamic therapy (SDT) can efficiently eliminate bacteria in deep bone tissue by generating reactive oxygen species (ROS).. This study presents a novel ROS antimicrobial-healing system (ZGPEG with Zn and Ga as layered double oxide) for treating bone infections.. SDT effectively eradicates biofilms and disrupts bacterial cell membranes.

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 hour until a reddish-purple precipitate indicative of TRAP activity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    Incubation:

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice.
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression.
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 houruntil a reddish-purple precipitate indicative of TRAPactivity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: The role of Lactobacillus rhamnosus in improving estrogen deficiency-induced osteoporosis by inhibiting NLRP3 inflammasome activation.
    Article Snippet: .. For tartrate-resistant acid phosphatase (TRAP) staining, deparaffinized and rehydrated sections were incubated with TRAP staining solution (Cat# G1050, Servicebio, China) at 37 ◦C in the dark for 50 min. After staining, sections were rinsed with distilled water and briefly counterstained with hematoxylin for 5 min, followed by the same dehydration, clearing, and mounting procedures described above. ..

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition
    Article Snippet: .. Based on our previous research [ ], RAW 264.7 cells were incubated with 100 μg/mL BTO and pBTO, and stimulated with 50 ng/mL RANKL (peprotech, USA) for 3–5 days and then subjected to TRAP staining (Servicebio, China). .. The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 hour until a reddish-purple precipitate indicative of TRAP activity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    Activity Assay:

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 hour until a reddish-purple precipitate indicative of TRAP activity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.



    Similar Products

    trap staining solution  (Servicebio Inc)
    86
    Servicebio Inc trap staining solution
    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pmc12670961-217-11-15
    Average 86 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    trap staining solution g1050  (Servicebio Inc)
    86
    Servicebio Inc trap staining solution g1050
    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution G1050, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pm41006700-274-12-19
    Average 86 stars, based on 1 article reviews
    trap staining solution g1050 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    trap staining solution  (Beyotime)
    99
    Beyotime trap staining solution
    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> <t>staining</t> in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
    Trap Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/DAPI+Staining+Solution/pmc12272599-57-23-42
    Average 99 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Bioactive Materials

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    doi: 10.1016/j.bioactmat.2025.10.040

    Figure Lengend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light.

    Techniques: Immunohistochemical staining, Staining, Immunofluorescence

    pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Journal: Materials Today Bio

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    doi: 10.1016/j.mtbio.2025.102050

    Figure Lengend Snippet: pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Article Snippet: The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Techniques: In Vitro, Staining

    pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Journal: Materials Today Bio

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    doi: 10.1016/j.mtbio.2025.102050

    Figure Lengend Snippet: pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Article Snippet: The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Techniques: In Vivo, Micro-CT, Microscopy, Staining