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trap staining solution  (Servicebio Inc)

 
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    Structured Review

    Servicebio Inc trap staining solution
    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pmc12670961-217-11-15
    Average 86 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1"

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.10.040

    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Figure Legend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Techniques Used: Immunohistochemical staining, Staining, Immunofluorescence

    Related Articles

    Staining:

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
    Article Snippet: .. Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light. ..

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice.
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression.
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 houruntil a reddish-purple precipitate indicative of TRAPactivity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: The role of Lactobacillus rhamnosus in improving estrogen deficiency-induced osteoporosis by inhibiting NLRP3 inflammasome activation.
    Article Snippet: .. For tartrate-resistant acid phosphatase (TRAP) staining, deparaffinized and rehydrated sections were incubated with TRAP staining solution (Cat# G1050, Servicebio, China) at 37 ◦C in the dark for 50 min. After staining, sections were rinsed with distilled water and briefly counterstained with hematoxylin for 5 min, followed by the same dehydration, clearing, and mounting procedures described above. ..

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition
    Article Snippet: .. Based on our previous research [ ], RAW 264.7 cells were incubated with 100 μg/mL BTO and pBTO, and stimulated with 50 ng/mL RANKL (peprotech, USA) for 3–5 days and then subjected to TRAP staining (Servicebio, China). .. The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 hour until a reddish-purple precipitate indicative of TRAP activity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    Incubation:

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice.
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression.
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 houruntil a reddish-purple precipitate indicative of TRAPactivity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    Article Title: Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice
    Article Snippet: .. For TRAP staining, dewaxed and rehydrated sections were incubated with TRAP staining solution (Cat# G1050; Servicebio) in the dark at 37 °C for 50 min, rinsed with distilled water, counterstained with hematoxylin for 5 min, and then subjected to the same dehydration, clearing, and mounting procedures as described above. ..

    Article Title: The role of Lactobacillus rhamnosus in improving estrogen deficiency-induced osteoporosis by inhibiting NLRP3 inflammasome activation.
    Article Snippet: .. For tartrate-resistant acid phosphatase (TRAP) staining, deparaffinized and rehydrated sections were incubated with TRAP staining solution (Cat# G1050, Servicebio, China) at 37 ◦C in the dark for 50 min. After staining, sections were rinsed with distilled water and briefly counterstained with hematoxylin for 5 min, followed by the same dehydration, clearing, and mounting procedures described above. ..

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition
    Article Snippet: .. Based on our previous research [ ], RAW 264.7 cells were incubated with 100 μg/mL BTO and pBTO, and stimulated with 50 ng/mL RANKL (peprotech, USA) for 3–5 days and then subjected to TRAP staining (Servicebio, China). .. The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 hour until a reddish-purple precipitate indicative of TRAP activity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.

    other:

    Article Title: Ultrasound activated Zn Ga layered double oxide as ROS antimicrobial-healing system boosts antimicrobial effect and infected bone regeneration
    Article Snippet: Bone infections are destructive diseases caused by microbial seeding, and sonodynamic therapy (SDT) can efficiently eliminate bacteria in deep bone tissue by generating reactive oxygen species (ROS).. This study presents a novel ROS antimicrobial-healing system (ZGPEG with Zn and Ga as layered double oxide) for treating bone infections.. SDT effectively eradicates biofilms and disrupts bacterial cell membranes.

    Activity Assay:

    Article Title: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression
    Article Snippet: .. TRAP staining solution (Servicebio, G1050) was added, and the samples were incubated at 37 °C in the dark for 1 hour until a reddish-purple precipitate indicative of TRAP activity became visible. .. TRAP-positive multinucleated cells were visualized and quantified under an ECLIPSE TE300 microscope (Nikon, Japan) to assess osteoclastogenesis.



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    trap staining solution  (Servicebio Inc)
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    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pmc12670961-217-11-15
    Average 86 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-10
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    trap staining solution g1050  (Servicebio Inc)
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    Servicebio Inc trap staining solution g1050
    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution G1050, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pm41006700-274-12-19
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    Image Search Results


    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Bioactive Materials

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    doi: 10.1016/j.bioactmat.2025.10.040

    Figure Lengend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light.

    Techniques: Immunohistochemical staining, Staining, Immunofluorescence