Journal: Bioactive Materials
Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
doi: 10.1016/j.bioactmat.2025.10.040
Figure Lengend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
Article Snippet: Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light.
Techniques: Immunohistochemical staining, Staining, Immunofluorescence