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exp2 stem trap column  (Optimize Technologies)


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    Optimize Technologies exp2 stem trap column
    Exp2 Stem Trap Column, supplied by Optimize Technologies, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+kit/EXP+2+Stem+Trap+Kit/pm42867249-80-18-22
    Average 94 stars, based on 4 article reviews
    exp2 stem trap column - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    other:

    Article Title: A size-exclusion-based approach for purifying extracellular vesicles from human plasma
    Article Snippet: EXP2 Stem Trap , Optimize Technologies , Part# 04003HNC18.

    Oligomer Restriction:

    Article Title: A High-Throughput Workflow for FFPE Tissue Proteomics
    Article Snippet: NanoLC-MS/MS acquisition and database searching The peptide digests were analyzed by nano LC-MS/MS on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific, Bremen, Germany) coupled to an UltiMate 3000 RSLCnano HPLC system with 0.1% formic acid in 98% water/2% acetonitrile for the solvent A and 0.1% formic acid in 80% acetonitrile/10% isopropanol/10% water for the solvent B. .. The peptide digests were solubilized in 0.1% formic acid and pumped onto a Halo C18 2.7 μm EXP2 stem trap (Optimize Technologies, Oregon City, OR) with 0.1% formic acid /0.05%TFA at a flow rate of 8 μl/ minute. .. The trap was placed in line with a 100 μm x 40 cm C18, 1.9 μm PepSep nano column (Bruker, Bremen, Germany) and the peptides separated at a flow rate of 350 nl/minute with a gradient of 3% to 35% solvent B over 120 minutes followed with a 10-minute ramp to 90% solvent B and hold for 10 minutes.

    Article Title: Metaproteomic analysis of human sonicate fluid highlights neutrophil extracellular trap formation associated with periprosthetic joint infection.
    Article Snippet: .. Peptides were resuspended in 0.2% formic acid, 0.1% trifluoroacetic acid, and 0.0001% Zwittergent 3-16 and loaded onto an EXP2 stem trap column (Optimize Technologies, Oregon City, OR), with subsequent separation on a 25 cm PepSep Extreme C18 column (25 cm × 150 μm × 1.5 μm C18 column, Bruker). ..

    Injection:

    Article Title: Conjugation of Synthetic N-Acetyl-Lactosamine to Azide-Containing Proteins Using the Staudinger Ligation
    Article Snippet: Generation of defined glycoconjugates is necessary for the study of glycoprotein function, as well as the development of therapeutics.. The biosynthesis of glycoproteins produces multiple glycoforms, proteins which differ only in the structure of the attached glycan.. This inherent heterogeneity complicates the study of isolated glycans and, in particular, could obscure the role of individual glycan epitopes in biological function.



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    Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of <t>H&E</t> <t>staining</t> (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and <t>TRAP</t> staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .
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    Image Search Results


    Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .

    Journal: International Dental Journal

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway

    doi: 10.1016/j.identj.2026.109417

    Figure Lengend Snippet: Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .

    Article Snippet: To detect multinucleated osteoclasts, tartrate-resistant acid phosphatase (TRAP) staining was performed with a TRAP kit (Servicebio).

    Techniques: Over Expression, Micro-CT, Staining, Immunohistochemical staining, Expressing, Control, Immunohistochemistry