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trap alp activity kit  (TaKaRa)


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    TaKaRa trap alp activity kit
    Trap Alp Activity Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+activity+kit/TRACP+and+ALP+Double-Stain+Kit/pm41466098-102-15-20
    Average 96 stars, based on 254 article reviews
    trap alp activity kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Tea extract increases cell fusion via regulation of cell surface DC-STAMP
    Article Snippet: .. Cells were cultured in a 96-well plate (2 × 10 3 cells/well) for 5 days, and then TRAP enzyme activity was measured using a TRAP activity kit (Takara Bio, Shiga, Japan), in accordance with the manufacturer's protocol. ..

    Activity Assay:

    Article Title: Tea extract increases cell fusion via regulation of cell surface DC-STAMP
    Article Snippet: .. Cells were cultured in a 96-well plate (2 × 10 3 cells/well) for 5 days, and then TRAP enzyme activity was measured using a TRAP activity kit (Takara Bio, Shiga, Japan), in accordance with the manufacturer's protocol. ..

    Article Title: Dioscorea polystachya Turcz. Extract Attenuates Osteoclastogenesis and Ovariectomy-Induced Bone Loss in Rats.
    Article Snippet: Ethnopharmacological relevance: Dioscorea polystachya Turcz. has been traditionally used in East Asian herbal medicine to strengthen bones and treat musculoskeletal conditions.. However, the pharmacological mechanisms underlying its anti-osteoporotic effects remain poorly defined.. Aim of the study: This study aimed to investigate the anti-osteoporotic potential of D. polystachya root extract (DRE) through its effects on osteoclast differentiation and function, using both in vitro and in vivo models.



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    Image Search Results


    The effect of TNFRSF11B-OE-CM on the differentiation of osteoclasts. THP-1 cells were incubated with PMA for 3 days, followed by culture in the presence of M-CSF (100 ng/mL), RANKL (100 ng/mL), and either control-CM or TNFRSF11B-OE-CM for an additional 12 days. (A, B) Representative images of TRAP staining and corresponding statistical analysis illustrate the number of TRAP-positive multinucleated cells with differentiated osteoclasts marked by red arrows. (C) TRAP activity was quantified in THP-1 cells treated with control-CM or TNFRSF11B-OE-CM. (D) qRT-PCR analysis was conducted to assess the expression levels of osteoclast differentiation-related marker genes in each group. Blank represents the uninduced group. Comparisons were made relative to the M-CSF and RANKL (M + R) stimulation group, # p < 0.05, ## p < 0.01; compared with the control-CM group, * p < 0.05; ** p < 0.01

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: TNFRSF11B-modified umbilical cord mesenchymal stem cells as a novel strategy for bone-related diseases by suppressing osteoclast activity

    doi: 10.1186/s13018-025-05850-9

    Figure Lengend Snippet: The effect of TNFRSF11B-OE-CM on the differentiation of osteoclasts. THP-1 cells were incubated with PMA for 3 days, followed by culture in the presence of M-CSF (100 ng/mL), RANKL (100 ng/mL), and either control-CM or TNFRSF11B-OE-CM for an additional 12 days. (A, B) Representative images of TRAP staining and corresponding statistical analysis illustrate the number of TRAP-positive multinucleated cells with differentiated osteoclasts marked by red arrows. (C) TRAP activity was quantified in THP-1 cells treated with control-CM or TNFRSF11B-OE-CM. (D) qRT-PCR analysis was conducted to assess the expression levels of osteoclast differentiation-related marker genes in each group. Blank represents the uninduced group. Comparisons were made relative to the M-CSF and RANKL (M + R) stimulation group, # p < 0.05, ## p < 0.01; compared with the control-CM group, * p < 0.05; ** p < 0.01

    Article Snippet: The measurement of TRAP activity utilized TRAP activity assay kits (Elabscience, Cat. No. E-BC-K871-M, Wuhan, China), with absorbance readings obtained at 405 nm via microplate reader.

    Techniques: Incubation, Control, Staining, Activity Assay, Quantitative RT-PCR, Expressing, Marker