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viral transfer plasmid plenticas9 blast  (Addgene inc)


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    Addgene inc viral transfer plasmid plenticas9 blast
    Viral Transfer Plasmid Plenticas9 Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfer+plasmid/lentiCas9-Blast+(Plasmid+%2352962)/bio_rxiv__64898__2026__03__30__714548-203-11-15
    Average 96 stars, based on 1658 article reviews
    viral transfer plasmid plenticas9 blast - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: METTL3–m 6 A–STAT1/NF-κB axis: a key switch for the immunosuppressive capacity of mesenchymal stem cells
    Article Snippet: Transfected cells were selected with 5 μg/mL puromycin (Gibco, USA). .. For lentiviral packaging, 293T cells in 10-cm dishes were transfected with Lipofectamine 2000 (36 μL) complexed for 20 min with 8 μg transfer plasmid, 6 μg psPAX2 (Addgene no. 12,260), and 2 μg pMD2.G (Addgene no. 12,259). ..

    Article Title: Alpha-synuclein overexpression triggers divergent cellular responses and post-translational modifications in SH-SY5Y and ReNcell VM models
    Article Snippet: .. HEK293T cells were transfected at ~ 70% confluency with the transfer plasmid (Addgene #36083 for eGFP, #40822 for WT α-syn, and #40823 for A53T α-syn), along with packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) in a 4:3:1 ratio. ..

    Article Title: Molecular insights into the regulation of GNPTαβ by LYSET.
    Article Snippet: Cells were transfected with individual overexpression plasmids (2 μg DNA for a 3.5cm2 dish) using either Lipofectamine 2000 (Invitrogen) or jetOPTIMUS transfection reagent from Polyplus (Sartorius) according to the manufacturer's protocol. .. Generation of lentiviral stable cell lines HEK293T cells were transfected with transfer plasmid, psPAX2 (Addgene 12260), and pMD2.G (Addgene 12259) at a 3.5:3.5:1 ratio using Lipofectamine 2000 according to the manufacturer's instructions. .. Generation of lentiviral stable cell lines HEK293T cells were transfected with transfer plasmid, psPAX2 (Addgene 12260), and pMD2.G (Addgene 12259) at a 3.5:3.5:1 ratio using Lipofectamine 2000 according to the manufacturer's instructions.

    Article Title: Alpha-synuclein overexpression triggers divergent cellular responses and post-translational modifications in SH-SY5Y and ReNcell VM models.
    Article Snippet: .. HEK293T cells were transfected at ~ 70% confluency with the transfer plasmid (Addgene #36083 for eGFP, #40822 for WT α‐syn, and #40823 for A53T α‐syn), along with packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) in a 4:3:1 ratio. ..

    Plasmid Preparation:

    Article Title: METTL3–m 6 A–STAT1/NF-κB axis: a key switch for the immunosuppressive capacity of mesenchymal stem cells
    Article Snippet: Transfected cells were selected with 5 μg/mL puromycin (Gibco, USA). .. For lentiviral packaging, 293T cells in 10-cm dishes were transfected with Lipofectamine 2000 (36 μL) complexed for 20 min with 8 μg transfer plasmid, 6 μg psPAX2 (Addgene no. 12,260), and 2 μg pMD2.G (Addgene no. 12,259). ..

    Article Title: Fine-tuning ERK activity enables proliferation-differentiation balance during lineage specification of human embryonic stem cells.
    Article Snippet: .. The transfer plasmid, psPAX2 (Addgene #12260), and pCMV-VSVG (Addgene #8454) were mixed at a ratio of 4:4:1 and incubated with 1 mg/mL of Polyethylene Glycol (PEI) (Polysciences, 24765-1) diluted in Opti-MEM for 25 min. ..

    Article Title: Alpha-synuclein overexpression triggers divergent cellular responses and post-translational modifications in SH-SY5Y and ReNcell VM models
    Article Snippet: .. HEK293T cells were transfected at ~ 70% confluency with the transfer plasmid (Addgene #36083 for eGFP, #40822 for WT α-syn, and #40823 for A53T α-syn), along with packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) in a 4:3:1 ratio. ..

    Article Title: Molecular insights into the regulation of GNPTαβ by LYSET.
    Article Snippet: Cells were transfected with individual overexpression plasmids (2 μg DNA for a 3.5cm2 dish) using either Lipofectamine 2000 (Invitrogen) or jetOPTIMUS transfection reagent from Polyplus (Sartorius) according to the manufacturer's protocol. .. Generation of lentiviral stable cell lines HEK293T cells were transfected with transfer plasmid, psPAX2 (Addgene 12260), and pMD2.G (Addgene 12259) at a 3.5:3.5:1 ratio using Lipofectamine 2000 according to the manufacturer's instructions. .. Generation of lentiviral stable cell lines HEK293T cells were transfected with transfer plasmid, psPAX2 (Addgene 12260), and pMD2.G (Addgene 12259) at a 3.5:3.5:1 ratio using Lipofectamine 2000 according to the manufacturer's instructions.

    Article Title: Alpha-synuclein overexpression triggers divergent cellular responses and post-translational modifications in SH-SY5Y and ReNcell VM models.
    Article Snippet: .. HEK293T cells were transfected at ~ 70% confluency with the transfer plasmid (Addgene #36083 for eGFP, #40822 for WT α‐syn, and #40823 for A53T α‐syn), along with packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) in a 4:3:1 ratio. ..

    Article Title: Fine-tuning ERK activity enables proliferation-differentiation balance during lineage specification of human embryonic stem cells
    Article Snippet: .. The transfer plasmid, psPAX2 (Addgene #12260), and pCMV-VSVG (Addgene #8454) were mixed at a ratio of 4:4:1 and incubated with 1 mg/mL of Polyethylene Glycol (PEI) (Polysciences, 24765-1) diluted in Opti-MEM for 25 min. ..

    Article Title: HPV16 E6 oncoprotein promotes microhomology-mediated viral integration by increasing PolΘ protein expression
    Article Snippet: Lentivirus was produced in HEK293T cells using the calcium phosphate transfection method (Takara Bio #631312). .. For each 10-cm plate, solution A was made with 12 μg transfer plasmid, 10 μg psPAX2 (Addgene #12260), 3 μg pMD2.G (Addgene #12259), 50 μL 2 M Calcium Solution, and 425 μL of H2O, and mixed with solution B (500 μL 2× HBS). ..

    Incubation:

    Article Title: Fine-tuning ERK activity enables proliferation-differentiation balance during lineage specification of human embryonic stem cells.
    Article Snippet: .. The transfer plasmid, psPAX2 (Addgene #12260), and pCMV-VSVG (Addgene #8454) were mixed at a ratio of 4:4:1 and incubated with 1 mg/mL of Polyethylene Glycol (PEI) (Polysciences, 24765-1) diluted in Opti-MEM for 25 min. ..

    Article Title: Fine-tuning ERK activity enables proliferation-differentiation balance during lineage specification of human embryonic stem cells
    Article Snippet: .. The transfer plasmid, psPAX2 (Addgene #12260), and pCMV-VSVG (Addgene #8454) were mixed at a ratio of 4:4:1 and incubated with 1 mg/mL of Polyethylene Glycol (PEI) (Polysciences, 24765-1) diluted in Opti-MEM for 25 min. ..

    Stable Transfection:

    Article Title: Molecular insights into the regulation of GNPTαβ by LYSET.
    Article Snippet: Cells were transfected with individual overexpression plasmids (2 μg DNA for a 3.5cm2 dish) using either Lipofectamine 2000 (Invitrogen) or jetOPTIMUS transfection reagent from Polyplus (Sartorius) according to the manufacturer's protocol. .. Generation of lentiviral stable cell lines HEK293T cells were transfected with transfer plasmid, psPAX2 (Addgene 12260), and pMD2.G (Addgene 12259) at a 3.5:3.5:1 ratio using Lipofectamine 2000 according to the manufacturer's instructions. .. Generation of lentiviral stable cell lines HEK293T cells were transfected with transfer plasmid, psPAX2 (Addgene 12260), and pMD2.G (Addgene 12259) at a 3.5:3.5:1 ratio using Lipofectamine 2000 according to the manufacturer's instructions.

    other:

    Article Title: HPV16 E6 oncoprotein promotes microhomology-mediated viral integration by increasing PolΘ protein expression.
    Article Snippet: Mutants of HPV16 E6, UBE3A, and POLQ were generated by site-directed mutagenesis using the plasmids mentioned above as the template.



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    (A) Cartoon describing the targets, binder types, and linkers used in this investigation. (B) sCELLISA data identifying candidate binders to Jurkat T cells. Each symbol represents the average of two fields of view from a biological replicate (n = 3), the bar represents the mean, and the error bars represent standard error of the mean. (C) Cartoon depicting the goal of evaluating sCELLISA hits via EV targeting. (D) Quantification of 15k (filled bars) and 120k (outlined bars) EVs targeted to Jurkat T cells with binders identified from the sCELLISA assay. The cellular fluorescence of each condition was background fluorescence subtracted (fluorescence attributable to cells only) and normalized by EV fluorescence determined by NanoFCM. Each bar graph represents an independent experiment, and each bar represents the mean of three biological replicates (n = 3). The error bars represent the standard error of the mean. To evaluate whether binders resulted in increased EV binding compared to the scaffold negative control, a two-way ANOVA was performed, and the Dunnett’s multiple comparison test is shown (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05). (E) Cartoon depicting evaluation of delivery improvement by candidate binders via lentivirus. (F) Quantification of functional titers of unconcentrated lentiviruses expressing mutant VSV-G and the binder indicated on the x-axis. Filled and outlined bars each represent an independent experiment. Each bar represents the mean functional titer as determined via linear regression by calculating the multiplicity of infection using the percent of Jurkat cells expressing dsRedExpress2 (also see Methods: <t>Lentiviral</t> Delivery Studies). Error bars represent the standard error of the slope of the linear regression. To identify if a binder improved delivery compared to the scaffold negative control, a pairwise comparison of each slope from each linear regression to the slope of the scaffold using a one-sample t-test was performed (***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05).
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    (A) Cartoon describing the targets, binder types, and linkers used in this investigation. (B) sCELLISA data identifying candidate binders to Jurkat T cells. Each symbol represents the average of two fields of view from a biological replicate (n = 3), the bar represents the mean, and the error bars represent standard error of the mean. (C) Cartoon depicting the goal of evaluating sCELLISA hits via EV targeting. (D) Quantification of 15k (filled bars) and 120k (outlined bars) EVs targeted to Jurkat T cells with binders identified from the sCELLISA assay. The cellular fluorescence of each condition was background fluorescence subtracted (fluorescence attributable to cells only) and normalized by EV fluorescence determined by NanoFCM. Each bar graph represents an independent experiment, and each bar represents the mean of three biological replicates (n = 3). The error bars represent the standard error of the mean. To evaluate whether binders resulted in increased EV binding compared to the scaffold negative control, a two-way ANOVA was performed, and the Dunnett’s multiple comparison test is shown (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05). (E) Cartoon depicting evaluation of delivery improvement by candidate binders via lentivirus. (F) Quantification of functional titers of unconcentrated lentiviruses expressing mutant VSV-G and the binder indicated on the x-axis. Filled and outlined bars each represent an independent experiment. Each bar represents the mean functional titer as determined via linear regression by calculating the multiplicity of infection using the percent of Jurkat cells expressing dsRedExpress2 (also see Methods: <t>Lentiviral</t> Delivery Studies). Error bars represent the standard error of the slope of the linear regression. To identify if a binder improved delivery compared to the scaffold negative control, a pairwise comparison of each slope from each linear regression to the slope of the scaffold using a one-sample t-test was performed (***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05).
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    (A) Cartoon describing the targets, binder types, and linkers used in this investigation. (B) sCELLISA data identifying candidate binders to Jurkat T cells. Each symbol represents the average of two fields of view from a biological replicate (n = 3), the bar represents the mean, and the error bars represent standard error of the mean. (C) Cartoon depicting the goal of evaluating sCELLISA hits via EV targeting. (D) Quantification of 15k (filled bars) and 120k (outlined bars) EVs targeted to Jurkat T cells with binders identified from the sCELLISA assay. The cellular fluorescence of each condition was background fluorescence subtracted (fluorescence attributable to cells only) and normalized by EV fluorescence determined by NanoFCM. Each bar graph represents an independent experiment, and each bar represents the mean of three biological replicates (n = 3). The error bars represent the standard error of the mean. To evaluate whether binders resulted in increased EV binding compared to the scaffold negative control, a two-way ANOVA was performed, and the Dunnett’s multiple comparison test is shown (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05). (E) Cartoon depicting evaluation of delivery improvement by candidate binders via lentivirus. (F) Quantification of functional titers of unconcentrated lentiviruses expressing mutant VSV-G and the binder indicated on the x-axis. Filled and outlined bars each represent an independent experiment. Each bar represents the mean functional titer as determined via linear regression by calculating the multiplicity of infection using the percent of Jurkat cells expressing dsRedExpress2 (also see Methods: Lentiviral Delivery Studies). Error bars represent the standard error of the slope of the linear regression. To identify if a binder improved delivery compared to the scaffold negative control, a pairwise comparison of each slope from each linear regression to the slope of the scaffold using a one-sample t-test was performed (***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05).

    Journal: bioRxiv

    Article Title: CELLISA – a cell-cell binding assay for evaluation of nanovesicle targeting proteins

    doi: 10.64898/2026.04.09.717595

    Figure Lengend Snippet: (A) Cartoon describing the targets, binder types, and linkers used in this investigation. (B) sCELLISA data identifying candidate binders to Jurkat T cells. Each symbol represents the average of two fields of view from a biological replicate (n = 3), the bar represents the mean, and the error bars represent standard error of the mean. (C) Cartoon depicting the goal of evaluating sCELLISA hits via EV targeting. (D) Quantification of 15k (filled bars) and 120k (outlined bars) EVs targeted to Jurkat T cells with binders identified from the sCELLISA assay. The cellular fluorescence of each condition was background fluorescence subtracted (fluorescence attributable to cells only) and normalized by EV fluorescence determined by NanoFCM. Each bar graph represents an independent experiment, and each bar represents the mean of three biological replicates (n = 3). The error bars represent the standard error of the mean. To evaluate whether binders resulted in increased EV binding compared to the scaffold negative control, a two-way ANOVA was performed, and the Dunnett’s multiple comparison test is shown (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05). (E) Cartoon depicting evaluation of delivery improvement by candidate binders via lentivirus. (F) Quantification of functional titers of unconcentrated lentiviruses expressing mutant VSV-G and the binder indicated on the x-axis. Filled and outlined bars each represent an independent experiment. Each bar represents the mean functional titer as determined via linear regression by calculating the multiplicity of infection using the percent of Jurkat cells expressing dsRedExpress2 (also see Methods: Lentiviral Delivery Studies). Error bars represent the standard error of the slope of the linear regression. To identify if a binder improved delivery compared to the scaffold negative control, a pairwise comparison of each slope from each linear regression to the slope of the scaffold using a one-sample t-test was performed (***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, p > 0.05).

    Article Snippet: The lentiviral transfer plasmid was grown in NEB Stable competent E. coli (New England Biolabs C3040) and grown at 30 °C.

    Techniques: Fluorescence, Binding Assay, Negative Control, Comparison, Functional Assay, Expressing, Mutagenesis, Infection