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External validation of TRAM1, TXNIP and ER stress-related hub genes (A) Validation of ER stress-related hub genes in the GSE60436 dataset. (B) The protein levels of TRAM1 and TXNIP were evaluated in cell samples by western blot. (C) The mRNA levels of BCL2, CCL2, IL-1Β, TLR4, TNF, and TP53 were measured in cell samples by qRT-PCR. Ctrl, control group; HG, high-glucose group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Journal: Frontiers in Endocrinology

Article Title: Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis

doi: 10.3389/fendo.2024.1341206

Figure Lengend Snippet: External validation of TRAM1, TXNIP and ER stress-related hub genes (A) Validation of ER stress-related hub genes in the GSE60436 dataset. (B) The protein levels of TRAM1 and TXNIP were evaluated in cell samples by western blot. (C) The mRNA levels of BCL2, CCL2, IL-1Β, TLR4, TNF, and TP53 were measured in cell samples by qRT-PCR. Ctrl, control group; HG, high-glucose group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The membranes were then blocked in QuickBlockTM Western Blocking Buffer (P0252, Beyotime) for 30 minutes and incubated overnight at 4°C with primary antibodies against TRAM1 (18243-1-AP, Proteintech) and TXNIP (12705-1-AP, Proteintech).

Techniques: Biomarker Discovery, Western Blot, Quantitative RT-PCR, Control

External validation of TRAM1, TXNIP and ER stress-related hub genes (A) Validation of ER stress-related hub genes in the GSE60436 dataset. (B) The protein levels of TRAM1 and TXNIP were evaluated in cell samples by western blot. (C) The mRNA levels of BCL2, CCL2, IL-1Β, TLR4, TNF, and TP53 were measured in cell samples by qRT-PCR. Ctrl, control group; HG, high-glucose group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Journal: Frontiers in Endocrinology

Article Title: Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis

doi: 10.3389/fendo.2024.1341206

Figure Lengend Snippet: External validation of TRAM1, TXNIP and ER stress-related hub genes (A) Validation of ER stress-related hub genes in the GSE60436 dataset. (B) The protein levels of TRAM1 and TXNIP were evaluated in cell samples by western blot. (C) The mRNA levels of BCL2, CCL2, IL-1Β, TLR4, TNF, and TP53 were measured in cell samples by qRT-PCR. Ctrl, control group; HG, high-glucose group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The membranes were then blocked in QuickBlockTM Western Blocking Buffer (P0252, Beyotime) for 30 minutes and incubated overnight at 4°C with primary antibodies against TRAM1 (18243-1-AP, Proteintech) and TXNIP (12705-1-AP, Proteintech).

Techniques: Biomarker Discovery, Western Blot, Quantitative RT-PCR, Control