Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: Comparison of molecular pathological markers in luteinized thecoma associated with sclerosing peritonitis and thecoma.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Comparison

Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: Molecular pathological markers show significant ascendant expression in LTSP. (A–F) Immunohistochemical staining of alpha-1,6-mannosylglycoprotein 6-beta- n -acetylglucosaminyltransferase B (MGAT5B), nuclear receptor coactivator 3 (NCOA3) and proliferation marker protein Ki-67 (MKI67) are respectively (A, C, E) diffusely positive in the luteinized cells but (B, D, F) weak to moderate in the thecoma cells. (G) Statistical analysis of the 3 markers. Data are expressed as mean ± SD. n = 9 to 11 sections per marker (G, LTSP); n = 81 to 85 sections per marker (G, Thecoma). *** P < .001 ( t test). Scale bar, 500 μm. AOD = average optical density, LTSP = luteinized thecoma associated with sclerosing peritonitis.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Expressing, Immunohistochemical staining, Staining, Marker

Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: MGAT5B-NCOA3 fusion gene in LTSP of the ovary. Schematic representation of the MGAT5B-NCOA3 fusion transcript including the exons and bases sequence involved. The breakpoint of the 5′ and 3′ partner genes are represented as red dotted lines. LTSP = luteinized thecoma associated with sclerosing peritonitis, MGAT5B = alpha-1,6-mannosylglycoprotein 6-beta- n -acetylglucosaminyltransferase B, NCOA3 = nuclear receptor coactivator 3.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Sequencing

Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: MGAT5B-NCOA3 fusion gene is most common in LTSP. (A–C) MGAT5B-NCOA3 fusion gene is most common in (A) luteinized cells, but (B) rare in thecoma cells and (C) normal fimbriae tubae cells as assessed by FISH. FISH showing fusion signals for MGAT5B-NCOA3 with the overlapping yellow fluorescent signal (white arrows: orange, NCOA3; green, MGAT5B; yellow, fusion area). (D) Statistical analysis of positive incidence. Data are expressed as mean ± SD percentage. n = 7 cases (D, LTSP); n = 5 cases (D, Thecoma); n = 2 cases (D, Control). *** P < .001 (one-way analysis of variance and post hoc test). Scale bar, 250 μm. FISH = fluorescence in situ hybridization, LTSP = luteinized thecoma associated with sclerosing peritonitis, MGAT5B = alpha-1,6-mannosylglycoprotein 6-beta- n -acetylglucosaminyltransferase B, NCOA3 = nuclear receptor coactivator 3, ns = no significance.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Control, Fluorescence, In Situ Hybridization

Journal: Frontiers in Endocrinology

Article Title: Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis

doi: 10.3389/fendo.2024.1341206

Figure Lengend Snippet: External validation of TRAM1, TXNIP and ER stress-related hub genes (A) Validation of ER stress-related hub genes in the GSE60436 dataset. (B) The protein levels of TRAM1 and TXNIP were evaluated in cell samples by western blot. (C) The mRNA levels of BCL2, CCL2, IL-1Β, TLR4, TNF, and TP53 were measured in cell samples by qRT-PCR. Ctrl, control group; HG, high-glucose group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The membranes were then blocked in QuickBlockTM Western Blocking Buffer (P0252, Beyotime) for 30 minutes and incubated overnight at 4°C with primary antibodies against TRAM1 (18243-1-AP, Proteintech) and TXNIP (12705-1-AP, Proteintech).

Techniques: Biomarker Discovery, Western Blot, Quantitative RT-PCR, Control

Journal: PLoS Pathogens

Article Title: Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway

doi: 10.1371/journal.ppat.1004881

Figure Lengend Snippet: (A) TLR2 follows a biphasic expression pattern during HCMV AD169 infection of NHDF fibroblasts with early induction and late down-regulation. Numbers below the TLR2 blot represent quantification of the protein signal. IB, immunoblot. TRAM1 was used as loading control. (B) miR-UL112-3p accumulates at late time points during AD169 infection of NHDF cells. miR-UL112-3p copy number was determined by RT-PCR at various points during the infection of NHDF fibroblasts with HCMV AD169 (MOI: 3). (C) TLR2 is down-regulated late during TB40E infection of THP-1 monocytic cells. (D) miR-UL112-3p accumulates at late time points during TB40E infection of differentiated THP-1 cells (MOI: 3).

Article Snippet: The following commercial antibodies were used: β-actin (#MAB1501R, Millipore), GAPDH (#ab8245, Abcam), IRAK1 (#PA5-17490, Pierce), TIRP/TICAM2 (#TA306163, Origene), TLR2 (#3268–1, Epitomics), MyD88 (#ab2064, Abcam) and TRAM1 (#TA308042, Origene).

Techniques: Expressing, Infection, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction

Journal: PLoS Pathogens

Article Title: Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection

doi: 10.1371/journal.ppat.1006397

Figure Lengend Snippet: A, B. Western blot analysis of proteins involved in TLR4 signaling pathway TRAM1, p-TRAM1, MyD88, IRAK4, p-IRAK4, Pyk2, p-Pyk2, p38, p-p38 in SupT1 cells and monocytes (A) or THP-1, THP-1 MyD88-/- and SupT1 cells (B) following stimulations with LPS, poly I:C, HPIV3/EboGP, HPIV3/ΔF-HN/EboGP, HPIV3 or EBOV (A) or empty beads or EBOV GP beads (B) in the presence or absence of the TLR4 inhibitor CLI-095. C , D , E . Western blot analysis of p65 phosphorylation in SupT1 cells (C), monocytes (D) or THP-1, THP-1 MyD88-/- and SupT1 cells (E) following stimulation with CD3/CD28 beads, LPS (+, 100 ng/ml, ++; 500 ng/ml), VLP (+, 100 μl; ++, 250 μl) and HPIV3/ΔF-HN/EboGP (+, MOI 0.1 PFU/cell; ++, MOI 1 PFU/cell), empty beads or EBOV GP beads, as indicated, with or without CLI-095. Western blots in panels A-E are representative of two independent experiments. F . Percentages of dead (Live/Dead + ) cells, cell positive for caspase 8 and 9 and proliferated CD4 + T lymphocytes following a 4 day-long incubation with EBOV or LPS with or without CLI-095. Mean values ±SE based on triplicates from one of two independent experiments with P values * P <0.05, ** P <0.01, n.s., non-significant (Student T-test).

Article Snippet: Membranes were blocked with 5% milk and 0.1% Tween-20 in PBS for 1 h at 37°C and stained with antibodies specific for the following molecules: TRAM1 (Abcam #ab96106), phosphorylated TRAM1 (FabGennix #PTRAM-140AP), MyD88 (#4283S), IRAK4 (#4363S), phosphorylated IRAK4 (#11927S), Pyk2 (#3090S), phosphorylated Pyk2 (#3291S), p38 (#8690S), phosphorylated p38 (#4511S), NFκB (#6956S), phosphorylated NFκB (#3033S) and GAPDH (#8884S) (all Cell Signaling Technology) diluted according manufacturer’s recommendations in PBS with 0.1% of Tween-20.

Techniques: Western Blot, Incubation