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tram 34 (MedChemExpress)

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Bulk RNA-Seq analysis of RAW 264.7 cells in vitro . (a) Unsupervised hierarchical clustering of DEGs (Log2FC, ∗ p < 0.05) associated with the immune response in RAW 264.7 cells after 14 days across various groups. (b – d) Representative KEGG enrichment analysis of key DEGs associated with biological process (BP), cellular component (CC), and molecular function (MF) in RAW 264.7 cells. (e, f) qRT-PCR validation of canonical M1 and M2 macrophage-specific gene markers expression in various groups at day 14 ( n = 5 each). (g) Unsupervised hierarchical clustering of DEGs (Log2FC, ∗ p < 0.05) associated with ion channel activity in RAW 264.7 cells after 14 days in various groups. (h) Pearson's correlation analysis of key DEGs associated with ion channel activity across groups. (i) Principal component analysis (PCA) analysis of variance in various treatment groups at day 14. (j, k) Fold change expression of Scn1b and KCa3.1 (= Kcnn4 ) (Log2FC, ∗ p < 0.05) associated with ion channel activity ( n = 5 each). (l, m) Representative immunostaining results for KCa3.1 (red) and Scn1b (blue) in various groups at day 14. Scale bar: 10 μm ( n = 5 each). (n, o) Immunostaining results with spatial fluoresence intensities for KCa3.1 and Stat6 in RAW 264.7 after ion channel inhibition using nifedipine (5 μM, L-type Ca 2+ ion channel <t>blocker),</t> <t>TRAM-34</t> (10 μM, Ca 2+ -activated K + blocker), and AS1810722 (10 μM, Stat6 blocker) at day 14. Scale bar: 10 μm. Data reported as mean ± s.d. of replicated experiments ( n = 5 each), statistical significance was considered at ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 (One-way ANOVA test with Tukey's test post hoc analysis).

Tram 34, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tram+34/TRAM-34/pmc13091342-375-29-32
Average 95 stars, based on 38 article reviews

tram 34 - by Bioz Stars, 2026-10

95/100 stars

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1) Product Images from "Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose"

Article Title: Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2026.03.038

Figure Legend Snippet: Bulk RNA-Seq analysis of RAW 264.7 cells in vitro . (a) Unsupervised hierarchical clustering of DEGs (Log2FC, ∗ p < 0.05) associated with the immune response in RAW 264.7 cells after 14 days across various groups. (b – d) Representative KEGG enrichment analysis of key DEGs associated with biological process (BP), cellular component (CC), and molecular function (MF) in RAW 264.7 cells. (e, f) qRT-PCR validation of canonical M1 and M2 macrophage-specific gene markers expression in various groups at day 14 ( n = 5 each). (g) Unsupervised hierarchical clustering of DEGs (Log2FC, ∗ p < 0.05) associated with ion channel activity in RAW 264.7 cells after 14 days in various groups. (h) Pearson's correlation analysis of key DEGs associated with ion channel activity across groups. (i) Principal component analysis (PCA) analysis of variance in various treatment groups at day 14. (j, k) Fold change expression of Scn1b and KCa3.1 (= Kcnn4 ) (Log2FC, ∗ p < 0.05) associated with ion channel activity ( n = 5 each). (l, m) Representative immunostaining results for KCa3.1 (red) and Scn1b (blue) in various groups at day 14. Scale bar: 10 μm ( n = 5 each). (n, o) Immunostaining results with spatial fluoresence intensities for KCa3.1 and Stat6 in RAW 264.7 after ion channel inhibition using nifedipine (5 μM, L-type Ca 2+ ion channel blocker), TRAM-34 (10 μM, Ca 2+ -activated K + blocker), and AS1810722 (10 μM, Stat6 blocker) at day 14. Scale bar: 10 μm. Data reported as mean ± s.d. of replicated experiments ( n = 5 each), statistical significance was considered at ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 (One-way ANOVA test with Tukey's test post hoc analysis).

Techniques Used: RNA Sequencing, In Vitro, Quantitative RT-PCR, Biomarker Discovery, Expressing, Activity Assay, Immunostaining, Inhibition

Incubation:

Article Title: Log-linear scaling of TRPV4-KCNN4 transcripts tunes ROCK-dependent mechanotransduction in a DCIS progression model
Article Snippet: .. During this incubation, cells received the following treatments in complete media: 1 nM GSK2193874 (GSK219, MedChemExpress HY-100720) for 1 hr, 5 μM TRAM-34 (MedChemExpress HY-13519) for 1 hr, 50 μM Y-27632 dihydrochloride (ROCK Inhibitor, Sigma Aldrich Y0503) for 1 hr, or Δ74.4 mOsm/L Polyethylene glycol 300 (PEG300, Millipore Sigma 8074845000) for 15 min. For combination treatments, when multiple treatments were performed within the same dish, the first treatment was applied for its full incubation time before addition of the second treatment: order of addition was as follows: 5 μM TRAM-34 followed by Δ74.4 mOsm/L PEG300, 50 μM ROCK Inhibitor followed by Δ74.4 mOsm/L PEG300, 1 nM GSK219 followed by Δ74.4 mOsm/L PEG300, or 1 nM GSK219 followed by 5 μM TRAM-34. ..

Article Title: Elevated K Ca 3.1 expression by angiotensin II via the ERK/NF-κB pathway contributes to atrial fibrosis.
Article Snippet: Atrial fibrillation (AF) is a prevalent cardiac arrhythmia characterized by atrial fibrosis which involves excessed proliferation and increased activity of fibroblast and myofibroblast, as well as alterations in the extracellular matrix (ECM).. The specific mechanism driving fibrosis in atrial fibroblasts and myofibroblsats remains incompletely understood.. This study investigates the role of the intermediate-conductance Ca2+-activated K channel (KCa3.1) in Angiotensin II (Ang II)-induced atrial fibrosis and elucidates the underlying mechanisms.

Solvent:

Saline:

Cell Culture:

Article Title: Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose
Article Snippet: .. For this, the RAW 264.7 cells (∼1.5 × 10 5 /mL/6-well) cultured with a-CNCs (100 μg mL −1 ) and ion channel inhibitors, such as Nifedipine (50 μM, Sigma-Aldrich), TRAM-34 (100 μM, MedChemExpress), and AS1810722 (100 μM, MedChemExpress) for 14 days. ..

other:

Article Title: Dysfunction in the SOCE/EDH-mediated vasorelaxation of mesenteric arteries impairs intestinal barrier integrity in irritable bowel syndrome.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

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Journal: Bioactive Materials

Article Title: Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose

doi: 10.1016/j.bioactmat.2026.03.038

Figure Lengend Snippet: Bulk RNA-Seq analysis of RAW 264.7 cells in vitro . (a) Unsupervised hierarchical clustering of DEGs (Log2FC, ∗ p < 0.05) associated with the immune response in RAW 264.7 cells after 14 days across various groups. (b – d) Representative KEGG enrichment analysis of key DEGs associated with biological process (BP), cellular component (CC), and molecular function (MF) in RAW 264.7 cells. (e, f) qRT-PCR validation of canonical M1 and M2 macrophage-specific gene markers expression in various groups at day 14 ( n = 5 each). (g) Unsupervised hierarchical clustering of DEGs (Log2FC, ∗ p < 0.05) associated with ion channel activity in RAW 264.7 cells after 14 days in various groups. (h) Pearson's correlation analysis of key DEGs associated with ion channel activity across groups. (i) Principal component analysis (PCA) analysis of variance in various treatment groups at day 14. (j, k) Fold change expression of Scn1b and KCa3.1 (= Kcnn4 ) (Log2FC, ∗ p < 0.05) associated with ion channel activity ( n = 5 each). (l, m) Representative immunostaining results for KCa3.1 (red) and Scn1b (blue) in various groups at day 14. Scale bar: 10 μm ( n = 5 each). (n, o) Immunostaining results with spatial fluoresence intensities for KCa3.1 and Stat6 in RAW 264.7 after ion channel inhibition using nifedipine (5 μM, L-type Ca 2+ ion channel blocker), TRAM-34 (10 μM, Ca 2+ -activated K + blocker), and AS1810722 (10 μM, Stat6 blocker) at day 14. Scale bar: 10 μm. Data reported as mean ± s.d. of replicated experiments ( n = 5 each), statistical significance was considered at ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 (One-way ANOVA test with Tukey's test post hoc analysis).

Article Snippet: For this, the RAW 264.7 cells (∼1.5 × 10 5 /mL/6-well) cultured with a-CNCs (100 μg mL −1 ) and ion channel inhibitors, such as Nifedipine (50 μM, Sigma-Aldrich), TRAM-34 (100 μM, MedChemExpress), and AS1810722 (100 μM, MedChemExpress) for 14 days.

Techniques: RNA Sequencing, In Vitro, Quantitative RT-PCR, Biomarker Discovery, Expressing, Activity Assay, Immunostaining, Inhibition

K + channel contributions to whole-cell current vary between cells adapted to 5 kPa or 18 kPa O 2 . ( A) Raw current traces under indicated conditions recorded from HUVEC exhibit outward rectifying whole-cell currents. ( B) Comparisons of normalised current amplitudes (relative to current at +50 mV) under indicated conditions show current inhibition relative to basal control from cells cultured at 5 kPa or 18 kPa using 10 mM TEA, 5 μM TRAM-34, 100 nM apamin (5 kPa: Basal vs. TEA: P = 0.0381, Basal vs. TEA + TRAM-34: P = 0.0095, Basal vs. TEA + TRAM-34+apamin: P = 0.0022; 18 kPa: Basal vs. TEA: P = 0.03723, Basal vs. TEA + TRAM-34: P = 0.0076, Basal vs. TEA + TRAM-34+apamin: P = 0.0042; two-way ANOVA, data denote mean ± S.E.M.). ( C ) Steady-state current-voltage (IV) relationships showing differential effects of TEA, TRAM-34 and apamin in HUVEC adapted to either O 2 level (5 kPa O 2 : F Treatment (3, 90) = 49.88, P < 0.0001, F Voltage (12, 90) = 131.0, P < 0.0001; 18 kPa O 2 : F Treatment (3, 104) = 15.50, P < 0.0001, F Voltage (12, 104) = 55.47, P < 0.0001, n = 2–3 cells each, two-way ANOVA, data denote mean (+S.E.M.).

Journal: Redox Biology

Article Title: Physiological oxygen levels reset K + channel activity in human vascular endothelial cells

doi: 10.1016/j.redox.2025.103981

Figure Lengend Snippet: K + channel contributions to whole-cell current vary between cells adapted to 5 kPa or 18 kPa O 2 . ( A) Raw current traces under indicated conditions recorded from HUVEC exhibit outward rectifying whole-cell currents. ( B) Comparisons of normalised current amplitudes (relative to current at +50 mV) under indicated conditions show current inhibition relative to basal control from cells cultured at 5 kPa or 18 kPa using 10 mM TEA, 5 μM TRAM-34, 100 nM apamin (5 kPa: Basal vs. TEA: P = 0.0381, Basal vs. TEA + TRAM-34: P = 0.0095, Basal vs. TEA + TRAM-34+apamin: P = 0.0022; 18 kPa: Basal vs. TEA: P = 0.03723, Basal vs. TEA + TRAM-34: P = 0.0076, Basal vs. TEA + TRAM-34+apamin: P = 0.0042; two-way ANOVA, data denote mean ± S.E.M.). ( C ) Steady-state current-voltage (IV) relationships showing differential effects of TEA, TRAM-34 and apamin in HUVEC adapted to either O 2 level (5 kPa O 2 : F Treatment (3, 90) = 49.88, P < 0.0001, F Voltage (12, 90) = 131.0, P < 0.0001; 18 kPa O 2 : F Treatment (3, 104) = 15.50, P < 0.0001, F Voltage (12, 104) = 55.47, P < 0.0001, n = 2–3 cells each, two-way ANOVA, data denote mean (+S.E.M.).

Article Snippet: TRAM-34 (5 μM) was from Cambridge Bioscience Ltd, UK.

Techniques: Inhibition, Control, Cell Culture

Journal: Redox Biology

Article Title: Physiological oxygen levels reset K + channel activity in human vascular endothelial cells

doi: 10.1016/j.redox.2025.103981

Figure Lengend Snippet: K + channel contributions to whole-cell current vary between cells adapted to 5 kPa or 18 kPa O 2 . ( A) Raw current traces under indicated conditions recorded from hCMEC/D3 cells exhibit outward rectifying whole-cell currents. ( B ) Comparisons of normalised current amplitudes (relative to current at +50 mV) under indicated conditions show current inhibition relative to basal control from cells cultured at 5 kPa or 18 kPa using 10 mM TEA, 5 μM TRAM-34, 100 nM apamin (5 kPa: Basal vs. TEA: P = 0.0375, Basal vs. TEA + TRAM-34: P = 0.0013, Basal vs. TEA + TRAM-34+apamin: P < 0.0001; 18 kPa: Basal vs. +TEA + TRAM-34+apamin: P = 0.0244, two-way ANOVA, data denote mean ± S.E.M.). ( C ) Steady-state current-voltage (IV) relationships showing differential effects of TEA, TRAM-34 and apamin in hCMEC/D3 cells adapted to either O 2 level (5 kPa O 2 : F Treatment (3, 102) = 173.0, P < 0.0001, F Voltage (12, 102) = 131.4, P < 0.0001; 18 kPa O 2 : F Treatment (3, 103) = 14.96, P < 0.0001, F Voltage (12, 103) = 38.59, P < 0.0001, n = 3 cells each, two-way ANOVA, data denote mean (+S.E.M.).

Article Snippet: TRAM-34 (5 μM) was from Cambridge Bioscience Ltd, UK.

Techniques: Inhibition, Control, Cell Culture

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tram 34 (MedChemExpress)

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1) Product Images from "Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose"

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