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total plasma srage  (R&D Systems)


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    R&D Systems total plasma srage
    Total Plasma Srage, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+rage/Human+RAGE+Quantikine+ELISA+Kit/pm37985201-75-0-8
    Average 95 stars, based on 121 article reviews
    total plasma srage - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Western Blot:

    Article Title: Neutrophil Cathepsin G and Tumor Cell RAGE Facilitate Neutrophil Anti-Tumor Cytotoxicity
    Article Snippet: .. Western blot analysis for RAGE expression in 4T1, AT3, and LLC cells using antibodies to N-terminal RAGE (clone A-9, Santa Cruz, RAGE-N), C-terminal RAGE (ab3611, Abcam, RAGE-C) or total RAGE (R&D, AF1179). .. Western blot analysis for RAGE expression in 4T1, AT3, and LLC cells using antibodies to N-terminal RAGE (clone A-9, Santa Cruz, RAGE-N), C-terminal RAGE (ab3611, Abcam, RAGE-C) or total RAGE (R&D, AF1179).

    Expressing:

    Article Title: Neutrophil Cathepsin G and Tumor Cell RAGE Facilitate Neutrophil Anti-Tumor Cytotoxicity
    Article Snippet: .. Western blot analysis for RAGE expression in 4T1, AT3, and LLC cells using antibodies to N-terminal RAGE (clone A-9, Santa Cruz, RAGE-N), C-terminal RAGE (ab3611, Abcam, RAGE-C) or total RAGE (R&D, AF1179). .. Western blot analysis for RAGE expression in 4T1, AT3, and LLC cells using antibodies to N-terminal RAGE (clone A-9, Santa Cruz, RAGE-N), C-terminal RAGE (ab3611, Abcam, RAGE-C) or total RAGE (R&D, AF1179).



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    Effect of acute exercise intensity on soluble receptor for advanced glycation endproducts <t>(sRAGE)</t> isoforms in lean healthy adults (LH) and adults with obesity (OB). Change in plasma concentrations of <t>total</t> <t>sRAGE</t> ( A ), endogenous secretory RAGE (esRAGE, B ), and cleaved RAGE (cRAGE, C ) were calculated by subtracting values obtained at baseline (Pre) from values obtained 30 min after (Post) treadmill exercise performed at 40% of participants’ V̇ o 2max for 60 min, 65% of V̇ o 2max for 30 min, or 80% of participants’ V̇ o 2max until calorie expenditure matched that which they obtained during the 40% exercise bout. Data are represented as means ± SD, and individual data points are plotted about the mean. Data were analyzed via 2-way ANOVA with Bonferroni correction used for post hoc analyses. P values for pairwise comparisons are shown when main effects approached significance ( P < 0.05). V̇ o 2max , maximal aerobic capacity.
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    Effect of acute exercise intensity on soluble receptor for advanced glycation endproducts (sRAGE) isoforms in lean healthy adults (LH) and adults with obesity (OB). Change in plasma concentrations of total sRAGE ( A ), endogenous secretory RAGE (esRAGE, B ), and cleaved RAGE (cRAGE, C ) were calculated by subtracting values obtained at baseline (Pre) from values obtained 30 min after (Post) treadmill exercise performed at 40% of participants’ V̇ o 2max for 60 min, 65% of V̇ o 2max for 30 min, or 80% of participants’ V̇ o 2max until calorie expenditure matched that which they obtained during the 40% exercise bout. Data are represented as means ± SD, and individual data points are plotted about the mean. Data were analyzed via 2-way ANOVA with Bonferroni correction used for post hoc analyses. P values for pairwise comparisons are shown when main effects approached significance ( P < 0.05). V̇ o 2max , maximal aerobic capacity.

    Journal: Journal of Applied Physiology

    Article Title: Soluble RAGE and skeletal muscle tissue RAGE expression profiles in lean and obese young adults across differential aerobic exercise intensities

    doi: 10.1152/japplphysiol.00748.2022

    Figure Lengend Snippet: Effect of acute exercise intensity on soluble receptor for advanced glycation endproducts (sRAGE) isoforms in lean healthy adults (LH) and adults with obesity (OB). Change in plasma concentrations of total sRAGE ( A ), endogenous secretory RAGE (esRAGE, B ), and cleaved RAGE (cRAGE, C ) were calculated by subtracting values obtained at baseline (Pre) from values obtained 30 min after (Post) treadmill exercise performed at 40% of participants’ V̇ o 2max for 60 min, 65% of V̇ o 2max for 30 min, or 80% of participants’ V̇ o 2max until calorie expenditure matched that which they obtained during the 40% exercise bout. Data are represented as means ± SD, and individual data points are plotted about the mean. Data were analyzed via 2-way ANOVA with Bonferroni correction used for post hoc analyses. P values for pairwise comparisons are shown when main effects approached significance ( P < 0.05). V̇ o 2max , maximal aerobic capacity.

    Article Snippet: Total sRAGE concentrations were measured in plasma samples by commercial ELISA (DRG00, R&D Systems, Minneapolis, MN) as per the manufacturer’s protocol.

    Techniques:

    Comparison of AGEs, sRAGE isoforms, and their ratios between healthy controls (CTR) and patients with type 2 diabetes

    Journal: Cardiovascular Diabetology

    Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

    doi: 10.1186/s12933-022-01535-3

    Figure Lengend Snippet: Comparison of AGEs, sRAGE isoforms, and their ratios between healthy controls (CTR) and patients with type 2 diabetes

    Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

    Techniques:

    Boxplots for the comparison of A total sRAGE, esRAGE, and cRAGE isoforms and ) AGEs/sRAGE, AGEs/esRAGE, AGEs/cRAGE, cRAGE/esRAGE ratio among healthy control subjects (CTR) and type 2 diabetes patients without (T2DM-NC) or with (T2DM-C) complications. **p < 0.01; ***p < 0.001; ****p < 0.0001 for Dunn’s post-hoc tests following Kruskal-Wallis H test

    Journal: Cardiovascular Diabetology

    Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

    doi: 10.1186/s12933-022-01535-3

    Figure Lengend Snippet: Boxplots for the comparison of A total sRAGE, esRAGE, and cRAGE isoforms and ) AGEs/sRAGE, AGEs/esRAGE, AGEs/cRAGE, cRAGE/esRAGE ratio among healthy control subjects (CTR) and type 2 diabetes patients without (T2DM-NC) or with (T2DM-C) complications. **p < 0.01; ***p < 0.001; ****p < 0.0001 for Dunn’s post-hoc tests following Kruskal-Wallis H test

    Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

    Techniques:

    Spearman correlations of AGEs and the different isoforms of sRAGE isoforms with age in healthy controls (CTR) and patients with type 2 diabetes

    Journal: Cardiovascular Diabetology

    Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

    doi: 10.1186/s12933-022-01535-3

    Figure Lengend Snippet: Spearman correlations of AGEs and the different isoforms of sRAGE isoforms with age in healthy controls (CTR) and patients with type 2 diabetes

    Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

    Techniques:

    Scatter plot showing the correlation between subjects’ age and circulating A cRAGE, B AGEs/sRAGE and C AGEs/cRAGE ratios. Regression lines for CTR (green) and type 2 diabetes (T2DM, red) individuals are displayed

    Journal: Cardiovascular Diabetology

    Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

    doi: 10.1186/s12933-022-01535-3

    Figure Lengend Snippet: Scatter plot showing the correlation between subjects’ age and circulating A cRAGE, B AGEs/sRAGE and C AGEs/cRAGE ratios. Regression lines for CTR (green) and type 2 diabetes (T2DM, red) individuals are displayed

    Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

    Techniques:

    Age- and HbA1c-adjusted multiple quantile regression model for the evaluation of AGEs and sRAGE isoforms in type 2 diabetes complications

    Journal: Cardiovascular Diabetology

    Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

    doi: 10.1186/s12933-022-01535-3

    Figure Lengend Snippet: Age- and HbA1c-adjusted multiple quantile regression model for the evaluation of AGEs and sRAGE isoforms in type 2 diabetes complications

    Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

    Techniques:

    Univariate and multivariate Cox regression analysis for the prediction of 15-year all-cause mortality in patients with type 2 diabetes

    Journal: Cardiovascular Diabetology

    Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

    doi: 10.1186/s12933-022-01535-3

    Figure Lengend Snippet: Univariate and multivariate Cox regression analysis for the prediction of 15-year all-cause mortality in patients with type 2 diabetes

    Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

    Techniques:

    The median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL) between non-diabetic, pre-diabetic and diabetic subjects.

    Journal: Nutrients

    Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

    doi: 10.3390/nu12123659

    Figure Lengend Snippet: The median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL) between non-diabetic, pre-diabetic and diabetic subjects.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

    Techniques: Concentration Assay

    Comparison of the median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL) in non-diabetes, pre-diabetes and diabetes, grouped in different ethnic groups.

    Journal: Nutrients

    Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

    doi: 10.3390/nu12123659

    Figure Lengend Snippet: Comparison of the median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL) in non-diabetes, pre-diabetes and diabetes, grouped in different ethnic groups.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

    Techniques: Comparison, Concentration Assay

    Ethnic comparison of median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL), grouped in non-diabetes, pre-diabetes and diabetes.

    Journal: Nutrients

    Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

    doi: 10.3390/nu12123659

    Figure Lengend Snippet: Ethnic comparison of median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL), grouped in non-diabetes, pre-diabetes and diabetes.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

    Techniques: Comparison, Concentration Assay

    The association factors of the different stages of development of type 2 diabetes with corrected serum α-tocopherol concentration, HOMA2 IR, HOMA2%B and serum RAGE concentration.

    Journal: Nutrients

    Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

    doi: 10.3390/nu12123659

    Figure Lengend Snippet: The association factors of the different stages of development of type 2 diabetes with corrected serum α-tocopherol concentration, HOMA2 IR, HOMA2%B and serum RAGE concentration.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

    Techniques: Concentration Assay

    Correlation of corrected serum α-tocopherol concentration with HOMA2 IR, HOMA2%B and serum RAGE Concentration.

    Journal: Nutrients

    Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

    doi: 10.3390/nu12123659

    Figure Lengend Snippet: Correlation of corrected serum α-tocopherol concentration with HOMA2 IR, HOMA2%B and serum RAGE Concentration.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

    Techniques: Concentration Assay

    Exosomes were isolated from plasma by ExoQuick precipitation method or by ultracentrifugation. Exosomes were lysed and analyzed for RAGE expression by Western blotting and representative blots are shown.

    Journal: Biochemical and biophysical research communications

    Article Title: Detection of the receptor for advanced glycation endproducts in neuronally-derived exosomes in plasma

    doi: 10.1016/j.bbrc.2018.04.181

    Figure Lengend Snippet: Exosomes were isolated from plasma by ExoQuick precipitation method or by ultracentrifugation. Exosomes were lysed and analyzed for RAGE expression by Western blotting and representative blots are shown.

    Article Snippet: RAGE Assay Total RAGE was measured in plasma and exosomes isolated from plasma using a commercially available ELISA that uses a monoclonal antibody raised against amino acids Gln24-Ala344 in the N-terminal extracellular domain of human RAGE (R&D Systems, Minneapolis, MN).

    Techniques: Isolation, Expressing, Western Blot

    L1CAM-postive and L1CAM-negative exosomes were isolated from plasma as detailed in the methods and analyzed for RAGE expression by Western blotting. A representative blot is shown.

    Journal: Biochemical and biophysical research communications

    Article Title: Detection of the receptor for advanced glycation endproducts in neuronally-derived exosomes in plasma

    doi: 10.1016/j.bbrc.2018.04.181

    Figure Lengend Snippet: L1CAM-postive and L1CAM-negative exosomes were isolated from plasma as detailed in the methods and analyzed for RAGE expression by Western blotting. A representative blot is shown.

    Article Snippet: RAGE Assay Total RAGE was measured in plasma and exosomes isolated from plasma using a commercially available ELISA that uses a monoclonal antibody raised against amino acids Gln24-Ala344 in the N-terminal extracellular domain of human RAGE (R&D Systems, Minneapolis, MN).

    Techniques: Isolation, Expressing, Western Blot