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tlr 7 8 ligand r848  (InvivoGen)


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    InvivoGen tlr 7 8 ligand r848
    Tlr 7 8 Ligand R848, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 2302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr 7 8 ligand r848/product/InvivoGen
    Average 97 stars, based on 2302 article reviews
    tlr 7 8 ligand r848 - by Bioz Stars, 2026-03
    97/100 stars

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    a. Schematic representation of the experimental design. MDDCs were exposed to uninfected (Jurkat, green) or HTLV-1-infected T cells (C91-PL, blue) for 24h, before restimulation with LPS or <t>R848</t> for an additional 24h. b. Flow cytometry analysis after CD11c and CD86 staining, in LPS- ( left ) or R848-restimulated MDDCs ( right ) pre-exposed to Jurkat (red) or C91-PL (dark blue) cells. Data are represented as the normalized MFI of CD86, with the MFI in restimulated Jurkat-pre-exposed MDDCs set to 100 (n = 30 or 10 independent experiments, respectively). Presented data are a subset of , respectively and were analysed with Kruskal-Wallis test or ordinary one-way ANOVA, respectively as described in and Tables c. Supernatant from the indicated cocultures was collected after LPS restimulation, and TNF-α ( left ) and IFN-I ( right ) concentrations were quantified for n = 3 or 10 independent experiments, respectively. Presented data are a subset of and were analysed with RM one-way ANOVA or ordinary one-way ANOVA, respectively as described in and Tables. d. Schematic drawing summarizing the results from Figs and . The drawing was created using BioRender.com .
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    Image Search Results


    Expression pattern of TLR-12 in the cycle of seminiferous epithelium. Anti-TLR-12 (NBP2-24,833, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-12. Immune-positive cells expressing TLR-12 ( a , b , c ) appear brown in color. TLR-12 is expressed in spermatocytes, round and elongated spermatids, endosomal compartments and acrosomes ( a , b , c ). Please note that acrosomes of round spermatids ( a , b ), endosomal compartments of both spermatocytes ( b , c ) and elongated spermatids ( c ) are immune positive. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-12 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ec endosomal compartment, rSt round spermatid, Ser Sertoli cell, *spermatocytes, and arrows indicate spermatogonia

    Journal: Histochemistry and Cell Biology

    Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

    doi: 10.1007/s00418-024-02310-z

    Figure Lengend Snippet: Expression pattern of TLR-12 in the cycle of seminiferous epithelium. Anti-TLR-12 (NBP2-24,833, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-12. Immune-positive cells expressing TLR-12 ( a , b , c ) appear brown in color. TLR-12 is expressed in spermatocytes, round and elongated spermatids, endosomal compartments and acrosomes ( a , b , c ). Please note that acrosomes of round spermatids ( a , b ), endosomal compartments of both spermatocytes ( b , c ) and elongated spermatids ( c ) are immune positive. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-12 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ec endosomal compartment, rSt round spermatid, Ser Sertoli cell, *spermatocytes, and arrows indicate spermatogonia

    Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

    Techniques: Expressing, Staining

    Expression pattern of TLR-13 in the cycle of seminiferous epithelium. Anti-TLR-13 (NBP2-24,539, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-13. Immune-positive cells expressing TLR-13 appear brown in color. TLR-13 is expressed only in elongated spermatids and residual bodies. Please note that the presence of two immune-positive residual bodies located at the luminal surface of the seminiferous of epithelium (arrows). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-13 is expressed at early stage of spermatogenesis. eSt elongated spermatid, Rb residual body, rSt round spermatid, *spermatocytes

    Journal: Histochemistry and Cell Biology

    Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

    doi: 10.1007/s00418-024-02310-z

    Figure Lengend Snippet: Expression pattern of TLR-13 in the cycle of seminiferous epithelium. Anti-TLR-13 (NBP2-24,539, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-13. Immune-positive cells expressing TLR-13 appear brown in color. TLR-13 is expressed only in elongated spermatids and residual bodies. Please note that the presence of two immune-positive residual bodies located at the luminal surface of the seminiferous of epithelium (arrows). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-13 is expressed at early stage of spermatogenesis. eSt elongated spermatid, Rb residual body, rSt round spermatid, *spermatocytes

    Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

    Techniques: Expressing, Staining

    a. Schematic representation of the experimental design. MDDCs were exposed to uninfected (Jurkat, green) or HTLV-1-infected T cells (C91-PL, blue) for 24h, before restimulation with LPS or R848 for an additional 24h. b. Flow cytometry analysis after CD11c and CD86 staining, in LPS- ( left ) or R848-restimulated MDDCs ( right ) pre-exposed to Jurkat (red) or C91-PL (dark blue) cells. Data are represented as the normalized MFI of CD86, with the MFI in restimulated Jurkat-pre-exposed MDDCs set to 100 (n = 30 or 10 independent experiments, respectively). Presented data are a subset of , respectively and were analysed with Kruskal-Wallis test or ordinary one-way ANOVA, respectively as described in and Tables c. Supernatant from the indicated cocultures was collected after LPS restimulation, and TNF-α ( left ) and IFN-I ( right ) concentrations were quantified for n = 3 or 10 independent experiments, respectively. Presented data are a subset of and were analysed with RM one-way ANOVA or ordinary one-way ANOVA, respectively as described in and Tables. d. Schematic drawing summarizing the results from Figs and . The drawing was created using BioRender.com .

    Journal: PLOS Pathogens

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells

    doi: 10.1371/journal.ppat.1012555

    Figure Lengend Snippet: a. Schematic representation of the experimental design. MDDCs were exposed to uninfected (Jurkat, green) or HTLV-1-infected T cells (C91-PL, blue) for 24h, before restimulation with LPS or R848 for an additional 24h. b. Flow cytometry analysis after CD11c and CD86 staining, in LPS- ( left ) or R848-restimulated MDDCs ( right ) pre-exposed to Jurkat (red) or C91-PL (dark blue) cells. Data are represented as the normalized MFI of CD86, with the MFI in restimulated Jurkat-pre-exposed MDDCs set to 100 (n = 30 or 10 independent experiments, respectively). Presented data are a subset of , respectively and were analysed with Kruskal-Wallis test or ordinary one-way ANOVA, respectively as described in and Tables c. Supernatant from the indicated cocultures was collected after LPS restimulation, and TNF-α ( left ) and IFN-I ( right ) concentrations were quantified for n = 3 or 10 independent experiments, respectively. Presented data are a subset of and were analysed with RM one-way ANOVA or ordinary one-way ANOVA, respectively as described in and Tables. d. Schematic drawing summarizing the results from Figs and . The drawing was created using BioRender.com .

    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.

    Techniques: Infection, Flow Cytometry, Staining