tlcv2 backbone (Addgene inc)
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Addgene inc
tlcv2 backbone
Tlcv2 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlcv2+backbone/TLCV2+(Plasmid+%2387360)/pmc12911328-37-7-9
Average 96 stars, based on 147 article reviews
Tlcv2 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlcv2+backbone/TLCV2+(Plasmid+%2387360)/pmc12911328-37-7-9
Average 96 stars, based on 147 article reviews
tlcv2 backbone - by Bioz Stars,
2026-10
96/100 stars
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Construct:Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3’ Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Polymerase Chain Reaction:Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3’ Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Amplification:Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3’ Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Article Title: In situ visualization of m 6 A sites in cellular mRNAs Article Snippet: .. To generate TLCV2-APO1-YTH, the APO1-YTH transgene was amplified from pCMV-APOBEC1-YTH (Addgene #131636) and inserted into the Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Article Title: Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer Progression Article Snippet: .. To construct a doxycycline (DOX)-inducible library, the Sequencing:Article Title: In situ visualization of m 6 A sites in cellular mRNAs Article Snippet: .. To generate TLCV2-APO1-YTH, the APO1-YTH transgene was amplified from pCMV-APOBEC1-YTH (Addgene #131636) and inserted into the Transfection:Article Title: TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos Article Snippet: Passage 2-5 PFFs were cultured in DMEM medium (Gibco) containing 10% (v/v) fetal bovine serum (Hyclone) for 72 h to induce contact inhibition, and digested by trypsin-EDTA solution to use as donor cells. .. For cell line establishment, PFFs were transfected with TDG-inducible expression vector by using the U023 program on Nucleofector 2b (Lonza), and then selected in culture medium containing 2 μg/mL puromycin for 7 d. The vector construction is based on Article Title: TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos Article Snippet: Passage 2-5 PFFs were cultured in DMEM medium (Gibco) containing 10% (v/v) fetal bovine serum (Hyclone) for 72 h to induce contact inhibition, and digested by trypsin-EDTA solution to use as donor cells. .. For cell line establishment, PFFs were transfected with TDG-inducible expression vector by using the U023 program on Nucleofector 2b (Lonza), and then selected in culture medium containing 2 μg/mL puromycin for 7 d. The vector construction is based on Expressing:Article Title: TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos Article Snippet: Passage 2-5 PFFs were cultured in DMEM medium (Gibco) containing 10% (v/v) fetal bovine serum (Hyclone) for 72 h to induce contact inhibition, and digested by trypsin-EDTA solution to use as donor cells. .. For cell line establishment, PFFs were transfected with TDG-inducible expression vector by using the U023 program on Nucleofector 2b (Lonza), and then selected in culture medium containing 2 μg/mL puromycin for 7 d. The vector construction is based on Article Title: TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos Article Snippet: Passage 2-5 PFFs were cultured in DMEM medium (Gibco) containing 10% (v/v) fetal bovine serum (Hyclone) for 72 h to induce contact inhibition, and digested by trypsin-EDTA solution to use as donor cells. .. For cell line establishment, PFFs were transfected with TDG-inducible expression vector by using the U023 program on Nucleofector 2b (Lonza), and then selected in culture medium containing 2 μg/mL puromycin for 7 d. The vector construction is based on Plasmid Preparation:Article Title: TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos Article Snippet: Passage 2-5 PFFs were cultured in DMEM medium (Gibco) containing 10% (v/v) fetal bovine serum (Hyclone) for 72 h to induce contact inhibition, and digested by trypsin-EDTA solution to use as donor cells. .. For cell line establishment, PFFs were transfected with TDG-inducible expression vector by using the U023 program on Nucleofector 2b (Lonza), and then selected in culture medium containing 2 μg/mL puromycin for 7 d. The vector construction is based on Article Title: Inhibition of Karyopherin β1-Mediated Nuclear Import Disrupts Oncogenic Lineage-Defining Transcription Factor Activity in Small Cell Lung Cancer Article Snippet: A gene fragment encoding the KPNB1 protein-coding region was made resistant to siRNA targeting by the introduction of silent mutations at all sequences targeted by the commercial KPNB1 siRNAs used (Karyopherin b1 siRNA(h) (Santa Cruz #sc-35736). .. The gene fragment was synthesized (GenScript) and inserted into the Article Title: TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos Article Snippet: Passage 2-5 PFFs were cultured in DMEM medium (Gibco) containing 10% (v/v) fetal bovine serum (Hyclone) for 72 h to induce contact inhibition, and digested by trypsin-EDTA solution to use as donor cells. .. For cell line establishment, PFFs were transfected with TDG-inducible expression vector by using the U023 program on Nucleofector 2b (Lonza), and then selected in culture medium containing 2 μg/mL puromycin for 7 d. The vector construction is based on Clone Assay:Article Title: CRISPR-based RNA-binding protein mapping in live cells. Article Snippet: RNA-binding proteins (RBPs) are involved in all aspects of RNA metabolism, and RNA-RBP interactions are important for cell homeostasis and viral replication.. The global RNA-binding proteome was recently reported; however, little is known about the proteins that bind to specific RNAs.. In this study, we describe a novel CRISPR-based RNA interaction proteomics method in live cells. Cloning:Article Title: CRISPR-based RNA-binding protein mapping in live cells. Article Snippet: RNA-binding proteins (RBPs) are involved in all aspects of RNA metabolism, and RNA-RBP interactions are important for cell homeostasis and viral replication.. The global RNA-binding proteome was recently reported; however, little is known about the proteins that bind to specific RNAs.. In this study, we describe a novel CRISPR-based RNA interaction proteomics method in live cells. Synthesized:Article Title: Inhibition of Karyopherin β1-Mediated Nuclear Import Disrupts Oncogenic Lineage-Defining Transcription Factor Activity in Small Cell Lung Cancer Article Snippet: A gene fragment encoding the KPNB1 protein-coding region was made resistant to siRNA targeting by the introduction of silent mutations at all sequences targeted by the commercial KPNB1 siRNAs used (Karyopherin b1 siRNA(h) (Santa Cruz #sc-35736). .. The gene fragment was synthesized (GenScript) and inserted into the |
