tubulin protein - fluorescent hilyte 647 porcine brain  (Cytoskeleton Inc)

Journal: bioRxiv

Article Title: Local fluidization of an active cytoplasmic gel partitions large cells

doi: 10.64898/2026.08.11.744254

Figure Lengend Snippet: a , Immunofluorescence images of a Xenopus laevis zygote fixed after the first mitosis, viewed from the animal pole. CPC (anti-INCENP-AF647) localized to the boundary between sister asters (microtubules imaged with anti-tubulin-AF488) and co-localized with an ER-depleted zone (ER labeled with anti-LNPK-AF568). b , Live images of a medaka embryo during 2-cell to 4-cell stage, showing dynamics of microtubules (HiLyte 647-tubulin), CPC (mCherry-Ol-Aurora B) and F-actin (Lifeact-EGFP) from mitosis to cytokinesis. c , CPC (anti-INCENP-AF647) localization in Xenopus egg extract co-imaged with microtubules (EB1-mApple), F-actin (Lifeact-GFP), and mitochondria (MitoView 405) at late anaphase. Top: untreated. Bottom: treated with 0.5 µM barasertib-HQPA. d , Example trajectories of two PEG-coated Fluoresbrite beads of 0.5 µm diameter in particle-tracking microrheology. Scale bar, 5 µm. e , Sampled areas in anaphase cycling egg extract with different cytoskeletal composition. f , Ensemble-averaged pairwise mean-squared displacement (MSD pair ) as a function of time lag τ for particles within each of the three indicated areas in e . g , Apparent diffusion coefficient (D) calculated from MSD pair between adjacent beads. For actin-intact controls, 8 areas (65x65 µm 2 ) in each category were sampled from independent experiments with 3 different egg extracts. The same extracts were used to prepare latrunculin B-added samples, where 20 areas were sampled in microtubule asters and cytoplasm, respectively. Data are mean ± SD. Also see . h , Local D calculated within overlapping 42x42 µm 2 grids, locally averaged, and overlaid onto microtubule images (EB1-mApple). Examples shown are microtubule asters growing against microtubule-free cytoplasm, in actin-intact egg extract (top) or extract treated with 10 µM LatB (bottom). Also see . All scale bars except d , 200 µm.

Article Snippet: HiLyte 647-tubulin (Cytoskeleton, Inc. TL670M) and mRNAs were mixed with final concentrations of 800 ng/μL for tubulin and150 ng/μL for each mRNA.

Techniques: Immunofluorescence, Labeling, Diffusion-based Assay

Journal: Cell reports

Article Title: Cisplatin resistance in an ovarian cancer model is mediated by microtubule dynamics regulator TPPP3 in synergy with tubulin code rewiring

doi: 10.1016/j.celrep.2026.117414

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Paclitaxel-stabilized cisplatin sensitive (CP-S) microtubules containing 97% CP-S tubulin, 1% biotin brain tubulin, and 2% 647-Hilyte tubulin (Cytoskeleton, cat no. TL670M-B) and paclitaxel-stabilized cisplatin resistant (CP-R) microtubules containing 99% CP-R tubulin, 1% biotin brain tubulin were immobilized on a glass surface using neutravidin (Life Technologies, A-2666).

Techniques: Cell-Signaling, Virus, Activation Assay, Control, Recombinant, Labeling, Mass Spectrometry, Sequencing, Cell Viability Assay, DNA Purification, Amplification, CRISPR, Plasmid Preparation, Synthesized, Software, Analysis, Filtration