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thp1 dualtm cells  (InvivoGen)


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    InvivoGen thp1 dualtm cells
    Thp1 Dualtm Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1+dualtm/THP1-Dual+Cells/pm42526606-77-0-2
    Average 98 stars, based on 424 article reviews
    thp1 dualtm cells - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    other:

    Article Title:
    Article Snippet: THP1-DualTM (monocytic leukemia cell) were purchased from InvivoGen (CA, US) were grown as indicated by the suppliers.

    Article Title: Secondary Immune Surveillance via upregulation of C-type lectin receptors
    Article Snippet: THP1-Dual (InvivoGen, USA) cell line was cultured in RPMI 1640 medium containing 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (30 min at 56 °C), 100 μg/ml Normocin and 100 U/ml Pen-Strep.

    Article Title: Nodamuravirus protein B2 boosts self-amplifying mRNA efficacy by overcoming innate immune barriers
    Article Snippet: Linearised plasmids were run on agarose electrophoresis and gel purified (Monarch DNA Gel extraction kit, New England Biolabs).

    Activity Assay:

    Article Title: Inhibitory Potential of the Drimane Sesquiterpenoids Isotadeonal and Polygodial in the NF-kB Pathway
    Article Snippet: The absorbance was measured by a microplate reader (VICTOR Nivo TM Perkin Elmer, Waltham, MA, USA) at 490 nm. .. THP1-DualTM (100.000 cells/well) were seeded in 96-well plates and were pretreated with different concentrations of polygodial and isotadeonal (from 1 to 50 μM) for 30 min, followed by the addition of LPS (100 ng/mL) for 18 h. Alkaline phosphatase activity was determined in cell supernatants using a QUANTI-Blue mixture (InvivoGen, Toulouse, France), according to the manufacturer’s specifications. .. THP1-DualTM (100.000 cells/well) were seeded in 96-well plates and were pretreated with different concentrations of polygodial and isotadeonal (from 1 to 50 μM) for 30 min, followed by the addition of LPS (100 ng/mL) for 18 h. Alkaline phosphatase activity was determined in cell supernatants using a QUANTI-Blue mixture (InvivoGen, Toulouse, France), according to the manufacturer’s specifications.

    Article Title: A modular mRNA–LNP vaccine platform enables integrated RNA, lipid and antigen design to protect against CCHFV
    Article Snippet: HEK293T (human embryonic kidney; ATCC CRL-11268), C2C12 (mouse myoblast; ATCC CRL-1772), HepG2 (human hepatocellular carcinoma; ATCC HB-8065), and ExpiCHO-S (Thermo Fisher Scientific, Cat# A29127) were used to assess mRNA–LNP cytotoxicity, expression, and protein production. .. HEK-BlueTM IFN-α/β (InvivoGen, Cat# hkb-ifnabv2), HEK-BlueTM IL-6 (InvivoGen, Cat# hkb-hil6), and THP1-DualTM (InvivoGen, Cat# thpd-nfis) were employed to assess type I interferon activity and innate immune activation in response to NPmut mRNA–LNPs formulated with BP-104, SM-102, or ALC-0315. ..

    Activation Assay:

    Article Title: A modular mRNA–LNP vaccine platform enables integrated RNA, lipid and antigen design to protect against CCHFV
    Article Snippet: HEK293T (human embryonic kidney; ATCC CRL-11268), C2C12 (mouse myoblast; ATCC CRL-1772), HepG2 (human hepatocellular carcinoma; ATCC HB-8065), and ExpiCHO-S (Thermo Fisher Scientific, Cat# A29127) were used to assess mRNA–LNP cytotoxicity, expression, and protein production. .. HEK-BlueTM IFN-α/β (InvivoGen, Cat# hkb-ifnabv2), HEK-BlueTM IL-6 (InvivoGen, Cat# hkb-hil6), and THP1-DualTM (InvivoGen, Cat# thpd-nfis) were employed to assess type I interferon activity and innate immune activation in response to NPmut mRNA–LNPs formulated with BP-104, SM-102, or ALC-0315. ..

    Suspension:

    Article Title: Benzo[b]selenophene sting regulator, preparation method and use thereof
    Article Snippet: .. THP1-dualTM (Invivogen: thpd-nfis) cells or RAW-LuciaTM (Invivogen: rawl-isg) cells were diluted using a culture medium, and 180 μL of cell suspension was sucked and inoculated in a 96-well plate, such that each well contain 1×105 cells. ..

    Stable Transfection:

    Article Title: Inhibitory Potential of the Drimane Sesquiterpenoids Isotadeonal and Polygodial in the NF-kB Pathway
    Article Snippet: Microglial cells (HMC-3) were obtained from the American Type Culture Collection (ATCC) and maintained in DMEM/High Glucose (Cytiva, Marlborough, UK) supplemented with 4 mM l-glutamine, 10% FBS, and 1% penicillin-streptomycin solution. .. THP1-DualTM (invivoGen, San Diego, CA, USA) cells are stably transfected with a secreted embryonic alkaline phosphatase gene (SEAP), which is under the control of an Molecules 2025, 30, 1555 9 of 14 NF-κB-inducible promoter. .. They were maintained in Roswell Park Memorial Institute RPMI-1640 medium (Corning, Corning, NY, USA) with 10% FBS, 10 nM HEPES, and 1% penicillin/streptomycin solution.

    Transfection:

    Article Title: Inhibitory Potential of the Drimane Sesquiterpenoids Isotadeonal and Polygodial in the NF-kB Pathway
    Article Snippet: Microglial cells (HMC-3) were obtained from the American Type Culture Collection (ATCC) and maintained in DMEM/High Glucose (Cytiva, Marlborough, UK) supplemented with 4 mM l-glutamine, 10% FBS, and 1% penicillin-streptomycin solution. .. THP1-DualTM (invivoGen, San Diego, CA, USA) cells are stably transfected with a secreted embryonic alkaline phosphatase gene (SEAP), which is under the control of an Molecules 2025, 30, 1555 9 of 14 NF-κB-inducible promoter. .. They were maintained in Roswell Park Memorial Institute RPMI-1640 medium (Corning, Corning, NY, USA) with 10% FBS, 10 nM HEPES, and 1% penicillin/streptomycin solution.

    Control:

    Article Title: Inhibitory Potential of the Drimane Sesquiterpenoids Isotadeonal and Polygodial in the NF-kB Pathway
    Article Snippet: Microglial cells (HMC-3) were obtained from the American Type Culture Collection (ATCC) and maintained in DMEM/High Glucose (Cytiva, Marlborough, UK) supplemented with 4 mM l-glutamine, 10% FBS, and 1% penicillin-streptomycin solution. .. THP1-DualTM (invivoGen, San Diego, CA, USA) cells are stably transfected with a secreted embryonic alkaline phosphatase gene (SEAP), which is under the control of an Molecules 2025, 30, 1555 9 of 14 NF-κB-inducible promoter. .. They were maintained in Roswell Park Memorial Institute RPMI-1640 medium (Corning, Corning, NY, USA) with 10% FBS, 10 nM HEPES, and 1% penicillin/streptomycin solution.



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    Image Search Results


    Co-localization shows direct interaction between bEV-located LPS and TLR4 and can be blocked by inhibiting TLR4. A Differentiated THP-1 cells were left untreated as control (Ctr) or stimulated with bacterial extracellular vesicles from Escherichia coli (bEV Ec ) for 1 h, fixed, and examined by proximity ligation assay (PLA) using TLR4 and LPS antibodies. Fluorescence images were acquired as z-stacks using a confocal laser scanning microscope. Red fluorescent dots show colocalization between TLR4 and LPS, while blue areas show DAPI-stained nuclei. Scale bar = 50 μm. Depicted are representative z-projections derived from corresponding z-stacks. B Quantification of PLA-positive fluorescence area divided by the number of nuclei per z-projection image from ( A ). Bars represent the mean values of three pictures per condition. (C + D) THP-1 Dual™ cells were stimulated with lipopolysaccharides (LPS) or bacterial extracellular vesicles from Legionella pneumophila (bEV Lp ) or bEV Ec for 20 h. Additionally, cells were pre-treated for 1 h either with (+) or without LPS-RS (LPS from Rhodobacter sphaeroides ), which acts antagonistically on TLR4. C Luciferase assay measuring the secreted IFN reporter from THP-1 Dual™ cells. Values are shown in relative luminescence units (RLU) obtained by using the QUANTI-Luc™ detection reagent. D ISG20 expression was determined by qPCR. Bars represent the mean values of three independent experiments + SEM. Statistics: Unpaired t-test comparing untreated control cells (Ctr) and bEV Ec -treated cells ( B ), or One-way ANOVA followed by Tukey’s multiple comparisons test with comparisons performed against the untreated control condition (Ctr) (C + D). Exact p-values are indicated in the graphs; n = 3. Symbols represent replicates B - D

    Journal: Cell Communication and Signaling : CCS

    Article Title: Bacterial extracellular vesicles modulate epithelial antiviral responses via macrophage-mediated immunomodulation

    doi: 10.1186/s12964-026-03003-x

    Figure Lengend Snippet: Co-localization shows direct interaction between bEV-located LPS and TLR4 and can be blocked by inhibiting TLR4. A Differentiated THP-1 cells were left untreated as control (Ctr) or stimulated with bacterial extracellular vesicles from Escherichia coli (bEV Ec ) for 1 h, fixed, and examined by proximity ligation assay (PLA) using TLR4 and LPS antibodies. Fluorescence images were acquired as z-stacks using a confocal laser scanning microscope. Red fluorescent dots show colocalization between TLR4 and LPS, while blue areas show DAPI-stained nuclei. Scale bar = 50 μm. Depicted are representative z-projections derived from corresponding z-stacks. B Quantification of PLA-positive fluorescence area divided by the number of nuclei per z-projection image from ( A ). Bars represent the mean values of three pictures per condition. (C + D) THP-1 Dual™ cells were stimulated with lipopolysaccharides (LPS) or bacterial extracellular vesicles from Legionella pneumophila (bEV Lp ) or bEV Ec for 20 h. Additionally, cells were pre-treated for 1 h either with (+) or without LPS-RS (LPS from Rhodobacter sphaeroides ), which acts antagonistically on TLR4. C Luciferase assay measuring the secreted IFN reporter from THP-1 Dual™ cells. Values are shown in relative luminescence units (RLU) obtained by using the QUANTI-Luc™ detection reagent. D ISG20 expression was determined by qPCR. Bars represent the mean values of three independent experiments + SEM. Statistics: Unpaired t-test comparing untreated control cells (Ctr) and bEV Ec -treated cells ( B ), or One-way ANOVA followed by Tukey’s multiple comparisons test with comparisons performed against the untreated control condition (Ctr) (C + D). Exact p-values are indicated in the graphs; n = 3. Symbols represent replicates B - D

    Article Snippet: The human cell lines THP-1 and THP-1 DualTM cells were obtained from the American Type Culture Collection and InvivoGen (Toulouse, France), respectively.

    Techniques: Control, Proximity Ligation Assay, Fluorescence, Laser-Scanning Microscopy, Staining, Derivative Assay, Luciferase, Expressing