monocytic cell line thp1  (ATCC)

Journal: Materials Today Bio

Article Title: Polyelectrolyte nanoparticles enable intracellular delivery of STING protein fragments for ovarian cancer immunotherapy

doi: 10.1016/j.mtbio.2026.103251

Figure Lengend Snippet: Polyanion component of nanoparticle allows for tuning cell specificity in STING activation. ( A ) Chemical structures of polyanions examined in nanoparticle formulations. ( B ) Z-Average diameter and polydispersity index (N = 8-13 independent formulations) as well as ( C ) zeta potential (N = 8-10 independent formulations) measurements of nanoparticles. (D) Fold-change in STING signaling activation compared to buffer 24 h after treatment with nanoparticle formulations at STINGΔTM doses of 5 μg/mL (RAW264.7), 8 μg/mL (HEK293T), 10 μg/mL (THP1), or 20 μg/mL (BPPNM and SKOV3). STING signaling measured as luminescent IRF3 reporter for RAW264.7, THP1, and HEK293T reporters or chemokine CXCL10 secretion in supernatant for SKOV3 and BPPNM. Each row represents one of N = 3 biological replicates, displaying the geometric mean of N = 3 technical replicates measuring the fold change in STING activation compared to the buffer control. Data represented as geometric mean ± standard deviation for data on log-scale and mean ± standard deviation for data on linear scale.

Article Snippet: RAW-Lucia ISG (rawl-isg), THP1-Dual (thpd-nfis), and HEK-Blue mTLR4 (hkb-mtlr4) reporter cells were purchased from InvivoGen.

Techniques: Activation Assay, Zeta Potential Analyzer, Control, Standard Deviation

Journal: Frontiers in Molecular Biosciences

Article Title: APOE3-Christchurch variant enhances neurovascular support functions of iPSC-derived mesenchymal stromal cells

doi: 10.3389/fmolb.2026.1778856

Figure Lengend Snippet: Generation and validation of iPSC-derived MSCs with APOE3 or APOE3Ch . (A) Fluorescence-Activated Cell Sorting (FACS) analysis confirming MSC identity showing positive markers CD73, CD90 and CD105 expressed in >95% of the cell population. (B) Quantification of APOE mRNA level by RT-qPCR, normalized to housekeeping gene GAPDH and expressed as fold change from the APOE3 iMSC group. Data shown as mean ± SEM (n = 3 independent differentiation batches per line). p > 0.05 by two-tailed unpaired Student’s t-test. (C) Densitometric of Western blot quantifying APOE levels in iMSC cell lysate and conditioned medium, expressed as fold change from the APOE3 iMSC group. Data shown as mean ± SEM (n = 3 independent differentiation batches per line). **p < 0.01 by two-tailed unpaired Student’s t-test. (D) Quantitative analysis of LPS-induced NF-kB reporter activity measured by luminescence in activated THP1 cells cultured alone (Con) or indirectly co-cultured with either APOE3 (+E3) or APOE3Ch (+E3Ch) iMSCs. Data was normalized to the +E3 condition and represented as mean ± SEM (n = 3 independent experiments, technical replicates from each experiment are represented by a distinct symbol, for display only). **p < 0.01, *p < 0.05 by mixed-effects model on n = 3 independent experiments with the Geisser-Greenhouse correction to account for matched values, and Tukey’s post hoc multiple comparisons test.

Article Snippet: THP1 NF-κB-Luc2 cells (THP1, ATCC, UK, Cat No. TIB-202-NFkB-LUC2) were thawed in complete THP1 media consisting of RPMI-1640 medium (Gibco) supplemented with 10% FBS (Thermo Scientific), 1 μg/mL puromycin (Fisher Scientific) and 0.05 mM 2-mercaptoethanol (Sigma-Aldrich) and cultured in suspension for 2 days.

Techniques: Biomarker Discovery, Derivative Assay, Fluorescence, FACS, Quantitative RT-PCR, Two Tailed Test, Western Blot, Activity Assay, Cell Culture