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monocytic cell line thp1  (ATCC)


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    ATCC monocytic cell line thp1
    Monocytic Cell Line Thp1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1+cell+lines/THP-1/pm42316966-59-9-16
    Average 99 stars, based on 20342 article reviews
    monocytic cell line thp1 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Luciferase:

    Article Title: lncRNA CARINH regulates expression and function of innate immune transcription factor IRF1 in macrophages
    Article Snippet: .. THP1 cell lines were obtained from the ATCC, and IRF-Lucia luciferase reporter monocytes (THP1-Lucia cells) and RAW-Lucia ISG cells (RAW-Lucia) were obtained from InvivoGen. .. All cell lines were authenticated using standard ATCC methods (morphology check by microscope, growth curve analysis) and tested monthly for mycoplasma contamination.

    Expressing:

    Article Title: Retrospective neutralization analysis of SARS-CoV-2 variants in early pandemic sera
    Article Snippet: .. HEK-293T and THP1 cell lines were all obtained from American Type Culture Collection (ATCC, Manassas, VA, United States), while ACE-2 and TMPRSS-2 expressing HEK293T (Hybrid-HEK293T) was purchased from GeneCopoeia (Rockville, MD, USA). .. Conventional HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St. Louis, MO, United States) containing 10% FBS, 1% L-glutamine and 1% penicillin-streptomycin.

    Cell Culture:

    Article Title: Fortilin binds and stabilizes MEF2C, activates it through phosphorylation, and drives transcription of the cell structural and survival protein CTNNA3.
    Article Snippet: .. 699 700 Cell culture and cell lines: We obtained HEK293T and THP1 cell lines from the 701 American Type Culture Collection (Manassas, VA, USA). .. HEK293T cells were 702 maintained in high-glucose Dulbecco's modified Eagle's medium (Corning, Corning, NY, 703 USA, Catalog #: 10-013-CV) supplemented with 10% fetal bovine serum (Sigma, 704 Catalog #: 21K475-A) and 1% Antibiotic-Antimycotic solution (GIBCO, Waltham, MA, 705 USA, Catalog #: 15240062) at 37°C in an atmosphere containing 5% CO2.

    Formulation:

    Article Title: Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation
    Article Snippet: Cell culture maintenance and shRNA depletion The THP1 cells (THP1 null; Invivogen) were a generous gift from the Griffin lab at Scripps Research and the ASC-GFP THP1 (Invivogen) cells were a generous gift from CALIBR. .. THP1 cell lines were maintained in RPMI 1640 (ATCC formulation, LifeTech cat. A1049101) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Lifetech cat. 10082147), 100 μg/ml Normocin (Invivogen cat. ant-nr-1) and Pen-Strep (100 μg/ml). .. Alternating passages of THP1 cells were treated with 200 μg/ml Hygromycin Gold (Invivogen cat. ant-hg-1) as recommended by Invivogen.

    Article Title: Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation
    Article Snippet: The THP1 cells (THP1 null; Invivogen) were a generous gift from the Griffin lab at Scripps Research and the ASC-GFP THP1 (Invivogen) cells were a generous gift from CALIBR. .. THP1 cell lines were maintained in RPMI 1640 (ATCC formulation, LifeTech cat. A1049101) supplemented with 10 % heat-inactivated fetal bovine serum (FBS) (Lifetech cat. 10082147), 100 μg/ml Normocin (Invivogen cat. ant-nr-1) and Pen-Strep (100 μg/ml). .. Alternating passages of THP1 cells were treated with 200 μg/ml Hygromycin Gold (Invivogen cat. ant-hg-1) as recommended by Invivogen.



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    A Confocal microscopy images of <t>THP1</t> cells stained with antibodies to H3K27ac (red) in nucleus (blue) after stimulation with insulin (top), and HDAC-inhibitor (bottom) for 24 h. Images are acquired with 40× magnification with additional digital magnification 1.5×. B Scatter plot of nuclear H3K27ac staining intensity after stimulation with insulin (top) and HDAC-inhibitor (bottom). P -values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001. C Histogram of phosphorylated serine 473 (p)AKT1 mean fluorescence intensity in THP1 cells stimulated with 10 nM insulin, by flow cytometry. D Histogram of H3K27ac mean fluorescence intensity in THP1 cells stimulated with increasing concentrations of insulin for 24 h, by flow cytometry. E Histogram of pAKT1 mean fluorescence intensity in human lymphocytes stimulated with increasing concentrations of insulin for 30 min, by flow cytometry.
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    A Confocal microscopy images of <t>THP1</t> cells stained with antibodies to H3K27ac (red) in nucleus (blue) after stimulation with insulin (top), and HDAC-inhibitor (bottom) for 24 h. Images are acquired with 40× magnification with additional digital magnification 1.5×. B Scatter plot of nuclear H3K27ac staining intensity after stimulation with insulin (top) and HDAC-inhibitor (bottom). P -values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001. C Histogram of phosphorylated serine 473 (p)AKT1 mean fluorescence intensity in THP1 cells stimulated with 10 nM insulin, by flow cytometry. D Histogram of H3K27ac mean fluorescence intensity in THP1 cells stimulated with increasing concentrations of insulin for 24 h, by flow cytometry. E Histogram of pAKT1 mean fluorescence intensity in human lymphocytes stimulated with increasing concentrations of insulin for 30 min, by flow cytometry.
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    A Confocal microscopy images of <t>THP1</t> cells stained with antibodies to H3K27ac (red) in nucleus (blue) after stimulation with insulin (top), and HDAC-inhibitor (bottom) for 24 h. Images are acquired with 40× magnification with additional digital magnification 1.5×. B Scatter plot of nuclear H3K27ac staining intensity after stimulation with insulin (top) and HDAC-inhibitor (bottom). P -values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001. C Histogram of phosphorylated serine 473 (p)AKT1 mean fluorescence intensity in THP1 cells stimulated with 10 nM insulin, by flow cytometry. D Histogram of H3K27ac mean fluorescence intensity in THP1 cells stimulated with increasing concentrations of insulin for 24 h, by flow cytometry. E Histogram of pAKT1 mean fluorescence intensity in human lymphocytes stimulated with increasing concentrations of insulin for 30 min, by flow cytometry.
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    A Confocal microscopy images of <t>THP1</t> cells stained with antibodies to H3K27ac (red) in nucleus (blue) after stimulation with insulin (top), and HDAC-inhibitor (bottom) for 24 h. Images are acquired with 40× magnification with additional digital magnification 1.5×. B Scatter plot of nuclear H3K27ac staining intensity after stimulation with insulin (top) and HDAC-inhibitor (bottom). P -values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001. C Histogram of phosphorylated serine 473 (p)AKT1 mean fluorescence intensity in THP1 cells stimulated with 10 nM insulin, by flow cytometry. D Histogram of H3K27ac mean fluorescence intensity in THP1 cells stimulated with increasing concentrations of insulin for 24 h, by flow cytometry. E Histogram of pAKT1 mean fluorescence intensity in human lymphocytes stimulated with increasing concentrations of insulin for 30 min, by flow cytometry.
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    A Confocal microscopy images of <t>THP1</t> cells stained with antibodies to H3K27ac (red) in nucleus (blue) after stimulation with insulin (top), and HDAC-inhibitor (bottom) for 24 h. Images are acquired with 40× magnification with additional digital magnification 1.5×. B Scatter plot of nuclear H3K27ac staining intensity after stimulation with insulin (top) and HDAC-inhibitor (bottom). P -values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001. C Histogram of phosphorylated serine 473 (p)AKT1 mean fluorescence intensity in THP1 cells stimulated with 10 nM insulin, by flow cytometry. D Histogram of H3K27ac mean fluorescence intensity in THP1 cells stimulated with increasing concentrations of insulin for 24 h, by flow cytometry. E Histogram of pAKT1 mean fluorescence intensity in human lymphocytes stimulated with increasing concentrations of insulin for 30 min, by flow cytometry.
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    Image Search Results


    A Confocal microscopy images of THP1 cells stained with antibodies to H3K27ac (red) in nucleus (blue) after stimulation with insulin (top), and HDAC-inhibitor (bottom) for 24 h. Images are acquired with 40× magnification with additional digital magnification 1.5×. B Scatter plot of nuclear H3K27ac staining intensity after stimulation with insulin (top) and HDAC-inhibitor (bottom). P -values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001. C Histogram of phosphorylated serine 473 (p)AKT1 mean fluorescence intensity in THP1 cells stimulated with 10 nM insulin, by flow cytometry. D Histogram of H3K27ac mean fluorescence intensity in THP1 cells stimulated with increasing concentrations of insulin for 24 h, by flow cytometry. E Histogram of pAKT1 mean fluorescence intensity in human lymphocytes stimulated with increasing concentrations of insulin for 30 min, by flow cytometry.

    Journal: Cell Death & Disease

    Article Title: Insulin enables acquisition of the IL7R + memory phenotype in PD1 + T cells in RA tissues

    doi: 10.1038/s41419-026-08916-6

    Figure Lengend Snippet: A Confocal microscopy images of THP1 cells stained with antibodies to H3K27ac (red) in nucleus (blue) after stimulation with insulin (top), and HDAC-inhibitor (bottom) for 24 h. Images are acquired with 40× magnification with additional digital magnification 1.5×. B Scatter plot of nuclear H3K27ac staining intensity after stimulation with insulin (top) and HDAC-inhibitor (bottom). P -values are calculated by Wilcoxon unpaired test. Asterisks indicate * < 0.05, ** < 0.01, *** <0.001, **** <0.0001. C Histogram of phosphorylated serine 473 (p)AKT1 mean fluorescence intensity in THP1 cells stimulated with 10 nM insulin, by flow cytometry. D Histogram of H3K27ac mean fluorescence intensity in THP1 cells stimulated with increasing concentrations of insulin for 24 h, by flow cytometry. E Histogram of pAKT1 mean fluorescence intensity in human lymphocytes stimulated with increasing concentrations of insulin for 30 min, by flow cytometry.

    Article Snippet: Human monocytic cell line THP1 (TIB-202, ATCC, Manassas, VA, USA) were propagated in RPMI medium (Gibco, Waltham, Massachusetts, USA) containing 50 μM β-mercaptoethanol (Gibco), Glutamax 2 mM (Gibco), sodium pyruvate 1 mM (Gibco), HEPES 10 mM (Gibco), gentamicin 50 μg/mL (Sanofi-Aventis) and 10% fetal bovine serum (Sigma-Aldrich) at 37 °C in a humidified 5% CO 2 atmosphere.

    Techniques: Confocal Microscopy, Staining, Fluorescence, Flow Cytometry