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cell lines  (ATCC)


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    Structured Review

    ATCC cell lines
    Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thle+3+cells/THLE-3/pm42236947-425-0-30
    Average 96 stars, based on 261 article reviews
    cell lines - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Epigenetic dysregulation of H19 / IGF2 in hepatic cells exposed to toxic metal mixtures in vitro
    Article Snippet: THLE-3, a cell line derived from normal adult hepatic epithelial cells, was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. THLE-3 cells were cultured in BEGM Bronchial Epithelial Cell Growth Basal Medium (Lonza, Walkersville, MD, USA) supplemented with 10% FBS (ATCC, Manassas, VA, USA), 1% antibiotic antimycotic solution (WELGENE Inc., Daegu, South Korea), 5 ng/mL human recombinant EGF (Corning Inc., Corning, NY, USA), 70 ng/mL phosphoethanolamine (Sigma Aldrich, St Louis, MO, USA), and the additives from the BEGM Bullet Kit (without gentamycin/amphotericin (GA) and epinephrine). ..

    Article Title: In vitro vascularized liver and tumor tissue microenvironments on a chip for dynamic determination of nanoparticle transport and toxicity
    Article Snippet: .. THLE-3 cells (ATCC, VA, CRL-11233) were cultured in BEGM Bullet Kit (Lonza) with additional 5 ng/ml epidermal growth factor (EGF; Invitrogen), 70 ng/ml phosphoethanolamine (Acros Organics, Geel, Belgium), and 10% FBS in a precoated flask. ..

    Article Title: Epigenetic dysregulation of H19/IGF2 in hepatic cells exposed to toxic metal mixtures in vitro.
    Article Snippet: THLE-3, a cell line derived from normal adult hepatic epithelial cells, was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. THLE-3 cells were cultured in BEGM Bronchial Epithelial Cell Growth Basal Medium (Lonza, Walkersville, MD, USA) supplemented with 10% FBS (ATCC, Manassas, VA, USA), 1% antibiotic antimycotic solution (WELGENE Inc., Daegu, South Korea), 5 ng/mL human recombinant EGF (Corning Inc., Corning, NY, USA), 70 ng/mL phosphoethanolamine (Sigma Aldrich, St Louis, MO, USA), and the additives from the BEGM Bullet Kit (without gentamycin/amphotericin (GA) and epinephrine). ..

    Article Title: Inhibition of miR-320 alleviates hepatic steatosis and dyslipidemia via suppressing the transcription of APOE in MASLD.
    Article Snippet: HepG2 (Cat #HB-8065, ATCC, Manassas, VA, USA), HEK293T (Cat #CRL-1573; ATCC, Manassas, VA, USA) and Huh-7 (Cat #ZQ0025, ZQXZBIO, Shanghai, China) cells were cultured in DMEM (Cat #11965092, Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Cat #A5670701, Gibco,Grand Island, NY, USA). .. THLE-3 cells (Cat #CRL-11233, ATCC; Manassas, VA, USA) were cultured in LHC-9 medium (Cat #12680013, Thermo Fisher Scientific, Waltham, MA, USA) containing 5 ng/mL EGF (Cat #PHG0313, Thermo Fisher Scientific, Waltham, MA, USA), 70 ng/mL phosphoethanolamine (Cat #P1348, Merck KGaA, Darmstadt, Germany), and 10% FBS (Cat #A5670701, Gibco, Grand Island, NY, USA). .. THLE-2 cells (Cat #ZQ1033,ZQXZBIO, Shanghai, China) were cultured in THLE-2 specific medium (Cat #ZQ-1340; ZQXZBIO, Shanghai, China).

    Article Title: Plasma extracellular vesicle microRNAs reflecting the therapeutic effect of the CBP/β-catenin inhibitor PRI-724 in patients with liver cirrhosis
    Article Snippet: C-82, the active form of PRI-724, was kindly provided by PRISM BioLab (Yokohama, Japan). .. The effect of C-82 in hepatocytes was analyzed using THLE-3 cells (CRL-3583, ATCC, USA), cultured in BEBM basal medium (CC-3171, Lonza, Switzerland) with 10% FBS, whereas the effect of C-82 in hepatic stellate cells was analyzed using the LX-2 Human Hepatic Stellate Cell Line (Sigma-Aldrich, Saint Louis, USA), cultured in DMEM with 2% FBS. .. LX-2 cells were exposed to human TGF-β1 (0.5 ng/ml) (R&D Systems, Minneapolis, USA) for 24 h before use.

    Article Title: Transcriptionally targeted and CpG-free plasmid for theranostic gene therapy
    Article Snippet: .. Cell Culture SK-HEP-1, Hep-3B, PC-3 and THLE-3 cells were purchased from the ATCC cell bank and cultured according the company's specifications for each line. .. Huh-7 cells were kindly provided by Dr. Tran's Lab at Johns Hopkins University and grown in high-glucose DMEM with 10% heat-inactivated FBS and 1% penicillin-streptomycin.

    Recombinant:

    Article Title: Epigenetic dysregulation of H19 / IGF2 in hepatic cells exposed to toxic metal mixtures in vitro
    Article Snippet: THLE-3, a cell line derived from normal adult hepatic epithelial cells, was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. THLE-3 cells were cultured in BEGM Bronchial Epithelial Cell Growth Basal Medium (Lonza, Walkersville, MD, USA) supplemented with 10% FBS (ATCC, Manassas, VA, USA), 1% antibiotic antimycotic solution (WELGENE Inc., Daegu, South Korea), 5 ng/mL human recombinant EGF (Corning Inc., Corning, NY, USA), 70 ng/mL phosphoethanolamine (Sigma Aldrich, St Louis, MO, USA), and the additives from the BEGM Bullet Kit (without gentamycin/amphotericin (GA) and epinephrine). ..

    Article Title: Epigenetic dysregulation of H19/IGF2 in hepatic cells exposed to toxic metal mixtures in vitro.
    Article Snippet: THLE-3, a cell line derived from normal adult hepatic epithelial cells, was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. THLE-3 cells were cultured in BEGM Bronchial Epithelial Cell Growth Basal Medium (Lonza, Walkersville, MD, USA) supplemented with 10% FBS (ATCC, Manassas, VA, USA), 1% antibiotic antimycotic solution (WELGENE Inc., Daegu, South Korea), 5 ng/mL human recombinant EGF (Corning Inc., Corning, NY, USA), 70 ng/mL phosphoethanolamine (Sigma Aldrich, St Louis, MO, USA), and the additives from the BEGM Bullet Kit (without gentamycin/amphotericin (GA) and epinephrine). ..



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    Image Search Results


    Nucleolar factors associated with FC/DFC are upregulated at both mRNA and protein level in HCC. Relative mRNA levels of ( A ) TCOF1 (Treacle), ( B ) UBF, ( C ) FBL (Fibrillarin), ( D ) NCL (nucleolin), and ( E ) NPM1 (nucleophosmin) measured by qPCR in HCC cells (PLC/PRF/5 and SNU-449) and control cells (THLE-3). GAPDH was used as the housekeeping gene, and fold change was determined relative to mRNA levels in THLE-3 cells. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD, with statistical significance assessed by one-way ANOVA and indicated with numerical P -values. Protein levels of ( F ) Treacle, ( G ) UBF, ( H ) Fibrillarin, ( I ) nucleolin, and ( J ) nucleophosmin measured by WB in HCC cells (PLC/PRF/5 and SNU-449) and control cells (THLE-3). α-tubulin was used as a loading control (LC). The molecular weight in kDa is listed on the left side of the blots (left panel), and protein signals were determined in each cell line relative to the loading control (right panel). Individual points in the graphs represent biological replicates ( n = 3). The graph depicts the mean ± SD, with statistical significance assessed by one-way ANOVA and indicated by numerical P -values.

    Journal: NAR Cancer

    Article Title: Ribosome biogenesis is increased in hepatocellular carcinoma and represents a potential therapeutic target

    doi: 10.1093/narcan/zcaf058

    Figure Lengend Snippet: Nucleolar factors associated with FC/DFC are upregulated at both mRNA and protein level in HCC. Relative mRNA levels of ( A ) TCOF1 (Treacle), ( B ) UBF, ( C ) FBL (Fibrillarin), ( D ) NCL (nucleolin), and ( E ) NPM1 (nucleophosmin) measured by qPCR in HCC cells (PLC/PRF/5 and SNU-449) and control cells (THLE-3). GAPDH was used as the housekeeping gene, and fold change was determined relative to mRNA levels in THLE-3 cells. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD, with statistical significance assessed by one-way ANOVA and indicated with numerical P -values. Protein levels of ( F ) Treacle, ( G ) UBF, ( H ) Fibrillarin, ( I ) nucleolin, and ( J ) nucleophosmin measured by WB in HCC cells (PLC/PRF/5 and SNU-449) and control cells (THLE-3). α-tubulin was used as a loading control (LC). The molecular weight in kDa is listed on the left side of the blots (left panel), and protein signals were determined in each cell line relative to the loading control (right panel). Individual points in the graphs represent biological replicates ( n = 3). The graph depicts the mean ± SD, with statistical significance assessed by one-way ANOVA and indicated by numerical P -values.

    Article Snippet: Human osteosarcoma U2OS cells (#HTB-96), HCC cell lines PLC/PRF/5 (#CRL-8024), SNU-449 (#CRL-2234), and normal liver epithelial cells THLE-3 (#CRL-3583) were purchased from the American Type Culture Collection.

    Techniques: Control, Molecular Weight

    Nucleolar expression of FC/DFC-associated factors is upregulated in HCC. Representative images of ( A ) Treacle (green), ( B ) UBF (green), ( C ) Fibrillarin (green), and ( D ) nucleolin (green) and nucleophosmin (magenta), and Pol II (yellow) in THLE-3, PLC/PRF/5, and SNU-449 cells. ( E ) Total nucleolar Treacle intensity per nucleus. Graphs depict one representative replicate ( n = 3) with data points representing individual cells and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for all three biological replicates combined, and statistical significance is indicated by numerical P -values. ( F ) Total nucleolar UBF intensity per nucleus otherwise as in panel (E). ( G ) Total nucleolar Fibrillarin intensity per nucleus, otherwise as in panel (E). ( H ) Total nucleolar nucleolin intensity per nucleus otherwise as in panel (E). (I) Total nucleolar nucleophosmin intensity per nucleus otherwise as in panel (E).

    Journal: NAR Cancer

    Article Title: Ribosome biogenesis is increased in hepatocellular carcinoma and represents a potential therapeutic target

    doi: 10.1093/narcan/zcaf058

    Figure Lengend Snippet: Nucleolar expression of FC/DFC-associated factors is upregulated in HCC. Representative images of ( A ) Treacle (green), ( B ) UBF (green), ( C ) Fibrillarin (green), and ( D ) nucleolin (green) and nucleophosmin (magenta), and Pol II (yellow) in THLE-3, PLC/PRF/5, and SNU-449 cells. ( E ) Total nucleolar Treacle intensity per nucleus. Graphs depict one representative replicate ( n = 3) with data points representing individual cells and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for all three biological replicates combined, and statistical significance is indicated by numerical P -values. ( F ) Total nucleolar UBF intensity per nucleus otherwise as in panel (E). ( G ) Total nucleolar Fibrillarin intensity per nucleus, otherwise as in panel (E). ( H ) Total nucleolar nucleolin intensity per nucleus otherwise as in panel (E). (I) Total nucleolar nucleophosmin intensity per nucleus otherwise as in panel (E).

    Article Snippet: Human osteosarcoma U2OS cells (#HTB-96), HCC cell lines PLC/PRF/5 (#CRL-8024), SNU-449 (#CRL-2234), and normal liver epithelial cells THLE-3 (#CRL-3583) were purchased from the American Type Culture Collection.

    Techniques: Expressing

    rDNA transcription is increased in HCC. ( A ) Representative images of EU incorporation (green). THLE-3, PLC/PRF/5, and SNU-449 cells were stained with DAPI (blue) and an antibody against Pol II (yellow). ( B ) Total nucleolar EU intensity per nucleus as a readout for rDNA transcription in THLE-3, PLC/PRF/5, and SNU-449 cells. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Nucleolar size measured through an inverse intensity-based mask of Pol II in THLE-3, PLC/PRF/5, and SNU-449 cells. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD, and statistical significance assessed by one-way ANOVA is indicated with numerical P -values. ( D ) Number of nucleoli per nucleus in THLE-3, PLC/PRF/5, and SNU-449 cells measured through an inverse intensity-based mask of Pol II. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD, and statistical significance assessed by one-way ANOVA is indicated with numerical P -values. ( E ) Total nucleolar area otherwise as in panel (C).

    Journal: NAR Cancer

    Article Title: Ribosome biogenesis is increased in hepatocellular carcinoma and represents a potential therapeutic target

    doi: 10.1093/narcan/zcaf058

    Figure Lengend Snippet: rDNA transcription is increased in HCC. ( A ) Representative images of EU incorporation (green). THLE-3, PLC/PRF/5, and SNU-449 cells were stained with DAPI (blue) and an antibody against Pol II (yellow). ( B ) Total nucleolar EU intensity per nucleus as a readout for rDNA transcription in THLE-3, PLC/PRF/5, and SNU-449 cells. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Nucleolar size measured through an inverse intensity-based mask of Pol II in THLE-3, PLC/PRF/5, and SNU-449 cells. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD, and statistical significance assessed by one-way ANOVA is indicated with numerical P -values. ( D ) Number of nucleoli per nucleus in THLE-3, PLC/PRF/5, and SNU-449 cells measured through an inverse intensity-based mask of Pol II. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD, and statistical significance assessed by one-way ANOVA is indicated with numerical P -values. ( E ) Total nucleolar area otherwise as in panel (C).

    Article Snippet: Human osteosarcoma U2OS cells (#HTB-96), HCC cell lines PLC/PRF/5 (#CRL-8024), SNU-449 (#CRL-2234), and normal liver epithelial cells THLE-3 (#CRL-3583) were purchased from the American Type Culture Collection.

    Techniques: Staining

    Anti-cancer drugs targeting the nucleolus inhibit cell viability in HCC. Drug response curves with corresponding IC 50 values and 95% CIs. Drugs were applied for 72 h, after which cell viability was assessed in THLE-3, PLC/PRF/5, and SNU-449 cells. ( A ) Drug response curve for CX-5461 with obtained IC 50 values and 95% CI (left panel). The graph shows the obtained IC 50 values represented as the mean ± SD (right panel). Individual points in the graph represent biological replicates ( n = 3). Statistical significance was assessed by one-way ANOVA and are indicated with numerical P -values. ( B ) Drug response curve for BMH-21 otherwise as in panel (A). ( C ) Drug response curve for Oxaliplatin, otherwise as in panel (A). ( D ) Drug response curve for JP-1302 otherwise as in panel (A). ( E ) Drug response curve for Sorafenib otherwise as in panel (A).

    Journal: NAR Cancer

    Article Title: Ribosome biogenesis is increased in hepatocellular carcinoma and represents a potential therapeutic target

    doi: 10.1093/narcan/zcaf058

    Figure Lengend Snippet: Anti-cancer drugs targeting the nucleolus inhibit cell viability in HCC. Drug response curves with corresponding IC 50 values and 95% CIs. Drugs were applied for 72 h, after which cell viability was assessed in THLE-3, PLC/PRF/5, and SNU-449 cells. ( A ) Drug response curve for CX-5461 with obtained IC 50 values and 95% CI (left panel). The graph shows the obtained IC 50 values represented as the mean ± SD (right panel). Individual points in the graph represent biological replicates ( n = 3). Statistical significance was assessed by one-way ANOVA and are indicated with numerical P -values. ( B ) Drug response curve for BMH-21 otherwise as in panel (A). ( C ) Drug response curve for Oxaliplatin, otherwise as in panel (A). ( D ) Drug response curve for JP-1302 otherwise as in panel (A). ( E ) Drug response curve for Sorafenib otherwise as in panel (A).

    Article Snippet: Human osteosarcoma U2OS cells (#HTB-96), HCC cell lines PLC/PRF/5 (#CRL-8024), SNU-449 (#CRL-2234), and normal liver epithelial cells THLE-3 (#CRL-3583) were purchased from the American Type Culture Collection.

    Techniques:

    Nucleolar-targeting compounds inhibit nucleolar activity and induce selective DNA damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA transcription in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).

    Journal: NAR Cancer

    Article Title: Ribosome biogenesis is increased in hepatocellular carcinoma and represents a potential therapeutic target

    doi: 10.1093/narcan/zcaf058

    Figure Lengend Snippet: Nucleolar-targeting compounds inhibit nucleolar activity and induce selective DNA damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA transcription in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).

    Article Snippet: Human osteosarcoma U2OS cells (#HTB-96), HCC cell lines PLC/PRF/5 (#CRL-8024), SNU-449 (#CRL-2234), and normal liver epithelial cells THLE-3 (#CRL-3583) were purchased from the American Type Culture Collection.

    Techniques: Activity Assay, Control, Staining