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Promega empty pgl3-basic plasmid
Empty Pgl3 Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+basic+pgl3+(empty)+plasmid/pgl3+basic/pm36403704-86-19-42
Average 90 stars, based on 1 article reviews
empty pgl3-basic plasmid - by Bioz Stars, 2026-10
90/100 stars

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other:

Article Title: Regulation of WNT16 in bone may involve upstream enhancers within CPED1
Article Snippet: For promoter assays, the empty vector was the pGL3-basic backbone (Promega) and the COL1A1-promoter (SP construct in ) was used as a positive control.

Article Title: Regulation of WNT16 in bone may involve upstream enhancers within CPED1.
Article Snippet: For promoter assays, the empty vector was the pGL3-basic backbone (Promega) and the COL1A1promoter (SP construct in30) was used as a positive control.

Article Title: Allele-specific expression identified rs2509956 as a novel long-distance cis -regulatory SNP for SCGB1A1 , an important gene for multiple pulmonary diseases.
Article Snippet: The empty vectors (pGL3-basic 176 and pGL3-promoter for segments containing rs3741240 and rs2509956, respectively) 177 were transfected as an external control and plasmid pRL-TK (25ng; Promega) was 178 co-transfected as an internal control in each transfection.

Article Title: Androgen receptor decreases the renal cell carcinoma bone metastases via suppressing the osteolytic formation through altering a novel circEXOC7 regulatory axis
Article Snippet: The sequencing of DHX9 5′‐promoter region was amplified by PCR and inserted into the empty pGL3‐basic vector (Promega, Madison, WI, USA) for luciferase (Luc) reporter assay.

Article Title: PLCz Functional Haplotypes Modulating Promoter Transcriptional Activity Are Associated with Semen Quality Traits in Chinese Holstein Bulls
Article Snippet: Cells were cotransfected with 50 ng of the pRL-TK vector DNA (Promega) and 400 ng of either the empty pGL3-Basic plasmid (a promoterless control from Promega) or one of the promoter constructs with different lengths and haplotypes of the PLCz promoter.

Expressing:

Article Title: Compositions comprising secreted extracellular vesicles of cells expressing NFATC4 useful for the treatment of cancer
Article Snippet: Cells were transiently transfected with the appropriate plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. .. Sequences of the Different Constructions: PGL3 basic (Promega, empty vector): SEQ ID NO:26; PGL3-hTGFß1-1670 promoter (expression of luciferase under control of hTGFß1 promoter): SEQ ID NO:27; Luciferase Assay Cells were cotransfected with either the PGL3 basic plasmid or the PGL3-hTGFß1-1670 Luciferase promoter construct and the pCS2-(n)-ß-galactosidase plasmid using Lipofectamine 2000. .. The following day cell were treated with either PBS or 1.109 particles/well in a 6 well plate for 24 h. After 24 h cell were lysed with the Reporter Lysis Buffer (Promega) and Luciferase and ß-gal activities were measured using the Luciferase Assay System (Promega) and Galacton-plus (Tropix) on a luminometer.

Luciferase:

Article Title: Compositions comprising secreted extracellular vesicles of cells expressing NFATC4 useful for the treatment of cancer
Article Snippet: Cells were transiently transfected with the appropriate plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. .. Sequences of the Different Constructions: PGL3 basic (Promega, empty vector): SEQ ID NO:26; PGL3-hTGFß1-1670 promoter (expression of luciferase under control of hTGFß1 promoter): SEQ ID NO:27; Luciferase Assay Cells were cotransfected with either the PGL3 basic plasmid or the PGL3-hTGFß1-1670 Luciferase promoter construct and the pCS2-(n)-ß-galactosidase plasmid using Lipofectamine 2000. .. The following day cell were treated with either PBS or 1.109 particles/well in a 6 well plate for 24 h. After 24 h cell were lysed with the Reporter Lysis Buffer (Promega) and Luciferase and ß-gal activities were measured using the Luciferase Assay System (Promega) and Galacton-plus (Tropix) on a luminometer.

Control:

Article Title: Compositions comprising secreted extracellular vesicles of cells expressing NFATC4 useful for the treatment of cancer
Article Snippet: Cells were transiently transfected with the appropriate plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. .. Sequences of the Different Constructions: PGL3 basic (Promega, empty vector): SEQ ID NO:26; PGL3-hTGFß1-1670 promoter (expression of luciferase under control of hTGFß1 promoter): SEQ ID NO:27; Luciferase Assay Cells were cotransfected with either the PGL3 basic plasmid or the PGL3-hTGFß1-1670 Luciferase promoter construct and the pCS2-(n)-ß-galactosidase plasmid using Lipofectamine 2000. .. The following day cell were treated with either PBS or 1.109 particles/well in a 6 well plate for 24 h. After 24 h cell were lysed with the Reporter Lysis Buffer (Promega) and Luciferase and ß-gal activities were measured using the Luciferase Assay System (Promega) and Galacton-plus (Tropix) on a luminometer.

Article Title: LRH-1/NR5A2 interacts with the glucocorticoid receptor to regulate glucocorticoid resistance.
Article Snippet: Nuclear receptors are transcription factors with important functions in a variety of physiological and pathological processes.. Targeting glucocorticoid receptor (GR) activity using glucocorticoids is a cornerstone in the treatment of patients with T cell acute lymphoblastic leukemia (T-ALL), and resistance to GC-induced cell death is associated with poor outcome and a high risk for relapse.. Next to ligand-binding, heterodimerization with other transcription factors presents an important mechanism for the regulation of GR activity.

Plasmid Preparation:

Article Title: Compositions comprising secreted extracellular vesicles of cells expressing NFATC4 useful for the treatment of cancer
Article Snippet: Cells were transiently transfected with the appropriate plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. .. Sequences of the Different Constructions: PGL3 basic (Promega, empty vector): SEQ ID NO:26; PGL3-hTGFß1-1670 promoter (expression of luciferase under control of hTGFß1 promoter): SEQ ID NO:27; Luciferase Assay Cells were cotransfected with either the PGL3 basic plasmid or the PGL3-hTGFß1-1670 Luciferase promoter construct and the pCS2-(n)-ß-galactosidase plasmid using Lipofectamine 2000. .. The following day cell were treated with either PBS or 1.109 particles/well in a 6 well plate for 24 h. After 24 h cell were lysed with the Reporter Lysis Buffer (Promega) and Luciferase and ß-gal activities were measured using the Luciferase Assay System (Promega) and Galacton-plus (Tropix) on a luminometer.

Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..

Article Title: LRH-1/NR5A2 interacts with the glucocorticoid receptor to regulate glucocorticoid resistance.
Article Snippet: Nuclear receptors are transcription factors with important functions in a variety of physiological and pathological processes.. Targeting glucocorticoid receptor (GR) activity using glucocorticoids is a cornerstone in the treatment of patients with T cell acute lymphoblastic leukemia (T-ALL), and resistance to GC-induced cell death is associated with poor outcome and a high risk for relapse.. Next to ligand-binding, heterodimerization with other transcription factors presents an important mechanism for the regulation of GR activity.

Construct:

Article Title: Compositions comprising secreted extracellular vesicles of cells expressing NFATC4 useful for the treatment of cancer
Article Snippet: Cells were transiently transfected with the appropriate plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. .. Sequences of the Different Constructions: PGL3 basic (Promega, empty vector): SEQ ID NO:26; PGL3-hTGFß1-1670 promoter (expression of luciferase under control of hTGFß1 promoter): SEQ ID NO:27; Luciferase Assay Cells were cotransfected with either the PGL3 basic plasmid or the PGL3-hTGFß1-1670 Luciferase promoter construct and the pCS2-(n)-ß-galactosidase plasmid using Lipofectamine 2000. .. The following day cell were treated with either PBS or 1.109 particles/well in a 6 well plate for 24 h. After 24 h cell were lysed with the Reporter Lysis Buffer (Promega) and Luciferase and ß-gal activities were measured using the Luciferase Assay System (Promega) and Galacton-plus (Tropix) on a luminometer.

Cotransfection:

Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..

Negative Control:

Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..



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Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

Journal: Genes

Article Title: Functional Analysis of Promoter Variants in Genes Involved in Sex Steroid Action, DNA Repair and Cell Cycle Control

doi: 10.3390/genes10030186

Figure Lengend Snippet: Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

Article Snippet: Similar experiments were performed with the empty promoterless pGL3-Basic plasmid (Promega) and the SV40-driven Firefly Luciferase pGL3-control plasmid (Promega, Madison, WI, USA) as negative and positive controls, respectively.

Techniques: Activity Assay, Luciferase, Plasmid Preparation, Clone Assay, Control, Expressing