Journal: Chinese Medicine

Article Title: Forsythoside A alleviates experimental autoimmune encephalomyelitis by targeting Tnfaip2

doi: 10.1186/s13020-026-01476-z

Figure Lengend Snippet: FA modulates the differentiation of peripheral Th cell subsets in EAE mice. A Proportion of Th1 cells in the spleen, determined by flow cytometry. B Proportion of Th17 cells in the spleen, determined by flow cytometry. C Proportion of Th2 cells in the spleen, determined by flow cytometry. Quantitative data are presented as mean ± SEM. * p < 0.05; ns not significant; Control, n = 4; EAE, n = 5; FA60, n = 5

Article Snippet: Staining procedures were performed using mouse Th1/Th2 and Th17 staining kits (Multi Sciences, China) according to the manufacturer's instructions.

Techniques: Flow Cytometry, Control

Journal: International Journal of Nanomedicine

Article Title: PLGA-PEG Nano-Adjuvant-Delivered ClfA Vaccine Elicits IL-17A-Mediated Neutrophil Activation to Confer Complete Protection Against Methicillin-Resistant Staphylococcus aureus

doi: 10.2147/IJN.S592819

Figure Lengend Snippet: Th17 cells play a crucial role in the clearance of S. aureus . ( a ) Schematic diagram of the mechanism of IL-17A in immune response. ( b ) Representative dot plots show the differences in the proportion of IL-17A⁺ cells within CD4⁺T cell subsets in splenic cells of mice in different treatment groups. ( c ) Statistical results of the expression level of IL-17A in spleen cells of mice in different treatment groups (n = 3). ( d ) Histograms of ROS levels in neutrophils in the peripheral blood of mice in different treatment groups. ( e ) Statistical results of ROS levels in neutrophils of peripheral blood of mice in different treatment groups (n = 3). ( f ) Representative dot plots show the proportion of ROS positive cells in neutrophils of mice in each group. All data are expressed as mean ± S.D. Statistical significance was established when p < 0.05 (ns, p ≥ 0.05; * p < 0.05; ** p < 0.01; **** p < 0.0001).

Article Snippet: Other reagents included: AlhydrogelTM adjuvant (Croda, UK); 96-well ELISA plates (Corning, NY, USA); ELISPOT kits (Mabtech, Stockholm, Sweden); flow cytometry antibodies—including PerCP-Cy5.5 anti-mouse Ly6G, APC anti-mouse CD3, PerCP anti-mouse CD4, and PE anti-mouse IL-17A (BioLegend, San Diego, USA); the Th17 inhibitor ( CZC24832 , MedChemExpress, NJ, USA); and a Reactive Oxygen Species (ROS) Detection Kit (Biyuntian, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Nanomedicine

Article Title: PLGA-PEG Nano-Adjuvant-Delivered ClfA Vaccine Elicits IL-17A-Mediated Neutrophil Activation to Confer Complete Protection Against Methicillin-Resistant Staphylococcus aureus

doi: 10.2147/IJN.S592819

Figure Lengend Snippet: Th17 inhibition leads to an increase in S. aureus organ load and a decrease in protection. Analysis of bacterial load in the ( a ) spleen, ( b ) liver, and ( c ) peripheral blood of mice from different groups 12 hours after S aureus infection (n = 6). ( d ) Survival rates on day 14 following a 1×LD 100 S. aureus challenge post-immunization (n = 10). All data are expressed as mean ± S.D. Statistical significance was established when p < 0.05 (ns, p ≥ 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; * ***p < 0.0001).

Article Snippet: Other reagents included: AlhydrogelTM adjuvant (Croda, UK); 96-well ELISA plates (Corning, NY, USA); ELISPOT kits (Mabtech, Stockholm, Sweden); flow cytometry antibodies—including PerCP-Cy5.5 anti-mouse Ly6G, APC anti-mouse CD3, PerCP anti-mouse CD4, and PE anti-mouse IL-17A (BioLegend, San Diego, USA); the Th17 inhibitor ( CZC24832 , MedChemExpress, NJ, USA); and a Reactive Oxygen Species (ROS) Detection Kit (Biyuntian, Shanghai, China).

Techniques: Inhibition, Infection