Journal: Scientific Reports

Article Title: Similarities and differences between the ‘cytokine storms’ in acute dengue and COVID-19

doi: 10.1038/s41598-020-76836-2

Figure Lengend Snippet: Serum cytokine concentrations in patients with varying severity of COVID-19. Cytokine and chemokine levels were measured by Luminex bead based Th9/Th17/Th 22 discovery performance assay in those who succumbed to their illness (n = 4), those who had severe pneumonia but survived (n = 4), those who had prolonged shedding (n = 7) and those with mild illness (n = 8), during day 4–9 ( time point A, n = 22) and during day 10–21 of illness (time point B, n = 22). Levels were also measured who had prolonged shedding and those with severe pneumonia during day 35–50 illness (time point C, n = 11) ( a – l ). The mean cytokine levels with SEM is shown. GM-CSF, IL-4, IL-12p70 and IL-17E were not within detectable range and not included in the figures.

Article Snippet: Concentration of seventeen analytes (CD40 Ligand, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-17E, IL-33, MIP-3α AND TNF-α) were assessed in serum samples by Luminex bead-based Th9/Th17/Th 22 discovery performance assay (R&D Systems, USA, Cat:LKTM009) according to manufacturer’s instructions.

Techniques: Luminex

Journal: Scientific Reports

Article Title: Similarities and differences between the ‘cytokine storms’ in acute dengue and COVID-19

doi: 10.1038/s41598-020-76836-2

Figure Lengend Snippet: Comparison of serum cytokine concentrations in patients with varying severity of COVID-19 and acute dengue. Cytokine and chemokine levels were measured by Luminex bead based Th9/Th17/Th 22 discovery performance assay in patients with COVID-19 who succumbed to their illness (n = 4), those who had severe pneumonia but survived (n = 4), those with mild illness (n = 8), during day 4 to 9 ( time point A, n = 22) and during day 10–21 of illness (time point B, n = 22) and also in patients with DF (n = 7) and DHF (n = 7) during early illness (time point A, ≤ 4 days of illness) and during the critical phase (time point B, day 5 to 7 of illness). The mean cytokine levels with SEM is shown.

Article Snippet: Concentration of seventeen analytes (CD40 Ligand, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-17E, IL-33, MIP-3α AND TNF-α) were assessed in serum samples by Luminex bead-based Th9/Th17/Th 22 discovery performance assay (R&D Systems, USA, Cat:LKTM009) according to manufacturer’s instructions.

Techniques: Comparison, Luminex

Journal: Scientific Reports

Article Title: Similarities and differences between the ‘cytokine storms’ in acute dengue and COVID-19

doi: 10.1038/s41598-020-76836-2

Figure Lengend Snippet: Serum cytokine concentrations in patients with varying severity of acute dengue. Cytokine and chemokine levels were measured by Luminex bead based Th9/Th17/Th 22 discovery performance assay in patients with DF (n = 7) and DHF (n = 7) during early illness (time point A, ≤ 4 days of illness) and during the critical phase (time point B, day 5 to 7 of illness) a–l . The mean cytokine levels with SEM is shown. GM-CSF, IL-4, IL-12p70 and IL-17E were not detected and therefore not shown. ** p < 0.001.

Article Snippet: Concentration of seventeen analytes (CD40 Ligand, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-17E, IL-33, MIP-3α AND TNF-α) were assessed in serum samples by Luminex bead-based Th9/Th17/Th 22 discovery performance assay (R&D Systems, USA, Cat:LKTM009) according to manufacturer’s instructions.

Techniques: Luminex

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Transcriptomic data suggest that Th17 cell/IL-17A-related signaling is involved in the Pasteurella multocida serotype A infection. A Principal component analysis (PCA) of the transcriptomic data of the PmCQ2 groups and Con groups. B Transcriptomic analysis of significantly different genes between the PmCQ2 groups and Con groups. C Gene Ontology (GO) analysis of PmCQ2 groups versus Con groups and the top 30 GO terms are shown. D Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PmCQ2 groups versus Con groups; the top 20 pathways are listed. E Th17 cell differentiation-associated genes identified via KEGG are shown. Red indicates upregulation and blue indicates downregulation. F Transcription levels of IL-6, IL-23, and TGF-β in PmCQ2-infected murine lungs at 24 hpi. G Representative images of mIHC staining of IL-17A and CD4 in Con- and PmCQ2-infected murine lungs at 32 hpi. The double-positive cells were mature Th17 cells. Scale bar = 100 μm. H Flow cytometry analysis of Th17 cells in PmCQ2-infected murine lungs at 32 hpi. (Right) quantification of Th17 cells in PmCQ2-infected murine lungs at 32 hpi. I Transcription levels of IL-17A and IL-22 in PmCQ2-infected murine lungs at 24 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Cell Differentiation, Staining, Flow Cytometry

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Th17 cells inhibit Pasteurella multocida serotype A infection. A Representative images of mIHC staining of the macrophage marker CD68 and the T-cell marker CD3 in Con- and PmCQ2-infected murine lungs at 32 hpi. Scale bar = 100 μm. B Flow cytometry analysis and quantification of CD3 + T cells in Con- and PmCQ2-infected murine lungs at 32 hpi. C A scheme showing the GSK805 (30 mg/kg) treatment protocol at the top. The survival curves of PmCQ2-infected mice treated with or without 30 mg/kg GSK805 are shown at the bottom. D A scheme presents the in vitro Th17 cell differentiation and treatment assay protocol. E Survival curves of PmCQ2-infected mice treated with or without 10 6 Th17 cells. F Representative photographs of murine lungs infected with PmCQ2 plus saline or Th17 cells at 32 hpi. G Representative images of HE staining of murine lungs infected with PmCQ2 plus saline or Th17 cells at 32 hpi. Scale bar = 200 μm. H The bacterial load of the infected lungs and blood at 32 hpi. I Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. J Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Staining, Marker, Flow Cytometry, In Vitro, Cell Differentiation, Saline

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: IL-17A, a Th17 cell effector, restricts Pasteurella multocida serotype A infection. A Quantification of IL-17A in PmCQ2-infected murine blood and lungs at 32 hpi. B Western blot analysis and quantification of IL-17A in PmCQ2-infected murine lungs. C A schematic of the mouse IL-17A neutralizing antibody treatment protocol. D Survival curves of PmCQ2-infected mice treated with or without 200 μg of mouse IL-17A neutralizing antibody. E The bacterial load of the infected lungs and blood at 32 hpi. F Representative photographs of murine lungs infected with PmCQ2 plus IgG or 200 μg of mouse IL-17A neutralizing antibody at 32 hpi. G Representative images of HE staining of murine lungs infected with PmCQ2 plus IgG or 200 μg of mouse IL-17A neutralizing antibody at 32 hpi. Scale bar = 200 μm. H Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. I Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. J A schematic of the 10 μg/kg recombinant mouse IL-17A treatment protocol. K Survival curves of PmCQ2-infected mice treated with or without 10 μg/kg recombinant mouse IL-17A. L Representative photographs of murine lungs infected with PmCQ2 plus 10 μg/kg BSA or 10 μg/kg recombinant mouse IL-17A at 32 hpi. M Representative images of HE staining of murine lungs infected with PmCQ2 plus 10 μg/kg BSA or 10 μg/kg recombinant mouse IL-17A at 32 hpi. Scale bar = 200 μm. N The bacterial load of the infected lungs and blood at 32 hpi. O Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. P Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Western Blot, Staining, Recombinant

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: STAT3 is critical for Th17 cell development during Pasteurella multocida serotype A infection. A Transcription levels of Stat3 in PmCQ2-infected murine lungs at 24 hpi. B Western blot analysis and quantification of p-Stat3 and Stat3 in PmCQ2-infected murine lungs. C A scheme presents the Stattic treatment assay protocol. D Western blot analysis and quantification of p-Stat3 and Stat3 in PmCQ2-infected murine lungs. E Survival curves of PmCQ2-infected mice treated with or without 15 mg/kg Stattic. F The bacterial load of the infected lungs and blood at 32 hpi. G Photographs of murine lungs infected with PmCQ2 plus vehicle or 15 mg/kg Stattic at 32 hpi. H Representative images of HE staining of murine lungs infected with PmCQ2 plus vehicle or 15 mg/kg Stattic at 32 hpi. Scale bar = 200 μm. I Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. J Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. K Western blot analysis and quantification of IL-17A in PmCQ2-infected murine lungs. L Representative images of mIHC staining of IL-17A and CD4 in Con- and PmCQ2-infected murine lungs at 32 hpi. The double-positive cells are mature Th17 cells. Scale bar = 100 μm. M Flow cytometry analysis and quantification of Th17 PmCQ2-infected murine lungs at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Western Blot, Staining, Flow Cytometry

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Regulation of Th17 cell/IL-17A activation via the IL-6–JAK2–STAT3 axis in Pasteurella multocida serotype A infection. A Transcription levels of IL-6, Jak2, and Stat3 in PmCQ2-infected murine lungs at 24 hpi. B Quantification of IL-6 in PmCQ2-infected murine serum in a time-dependent manner. C Western blot analysis and quantification of p-Jak2, Jak2, p-Stat3, and Stat3 in PmCQ2-infected murine lungs in a time-dependent manner. D A schematic of the IL-6-KO mouse treatment protocol at the top. The survival curves of PmCQ2-infected IL-6-KO mice treated with or without 10 μg/kg recombinant mouse IL-17A are shown. E Western blot analysis and quantification of p-Jak2, Jak2, p-Stat3, and Stat3 in PmCQ2-infected murine lungs. F Representative images of mIHC staining of IL-17A and CD4 in Con- and PmCQ2-infected murine lungs at 32 hpi. The double-positive cells are mature Th17 cells. Scale bar = 100 μm. G Flow cytometry analysis and quantification of Th17 PmCQ2-infected murine lungs at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Activation Assay, Infection, Western Blot, Recombinant, Staining, Flow Cytometry

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Schematic showing that Th17 cells/IL-17A shape Pasteurella multocida serotype A infection. Serotype A Pasteurella multocida (PmA) causes systemic infection and excessive release of inflammatory factors by destroying the lung barrier. In contrast to previous reports that PmA induces an excessive immune response, although PmA can induce a Th17 cell response, it inhibits T-cell immunity, resulting in insufficient activation of Th17 cells and their effector molecule IL-17A, which ultimately limits the clearance of PmA. At the molecular level, the IL-6–JAK2–STAT3 axis is strongly involved in Th17 cell/IL-17A activation during PmA infection. Importantly, targeting Th17 cells and IL-17A has potential for clinical application, as evidenced by the significant attenuation of PmA-induced lung injury and systemic inflammation as well as the reduction in animal mortality.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Activation Assay

Journal: Foods

Article Title: Echinacea purpurea (L.) Moench Polysaccharide Alleviates DSS-Induced Colitis in Rats by Restoring Th17/Treg Balance and Regulating Intestinal Flora

doi: 10.3390/foods12234265

Figure Lengend Snippet: Effect of EPP on Th17/Treg balance. ( A ) Flow cytometry of the spleen and mesenteric lymph nodes. ( B ) Ratio of Th17 and Treg cells in the spleen and mesenteric lymph nodes. ( C ) Detection of genes and cytokines associated with Th17/Treg balance. * A significant difference compared with the control group, p < 0.05. ** A highly significant difference compared with the control group, p < 0.01. # A significant difference compared with the model group, p < 0.05. ## A highly significant difference compared with the model group, p < 0.01.

Article Snippet: For CD4 + , CD25 + , IL-17, and forkhead transcription factor protein 3 (Foxp3) staining, the cells were washed, fixed, and then stained using a flow cytometry staining kit (Elabscience, Wuhan, China).

Techniques: Flow Cytometry, Control

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Comparison of Th17 cells, Treg cells, and Th17/Treg ratios between stroke patients and HCs.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques: Comparison

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Serum RBP4 was positively associated with a Th17/Treg imbalance. Association of serum RBP4 with Th17 cells (A) , Treg cells (B) , and the Th17/Treg ratio (C) in stroke patients. Association of serum RBP4 with Th17 cells (D) , Treg cells (E) , and the Th17/Treg ratio (F) in HCs.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques:

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Serum RBP4 and Th17/Treg imbalances were negatively associated with the MMSE score at enrollment. Association of serum RBP4 (A) , Th17 cells (B) , Treg cells (C) , and the Th17/Treg ratio (D) with MMSE score at enrollment in stroke patients.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques:

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Serum RBP4 and Th17/Treg imbalances were associated with the occurrence of cognitive impairment at enrollment. Association of serum RBP4 (A) , Th17 cells (B) , Treg cells (C) , and the Th17/Treg ratio (D) with cognitive impairment at enrollment in stroke patients.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques:

Journal: The FASEB Journal

Article Title: Periodontitis Salivary Microbiota Exacerbates Murine Rheumatoid Arthritis via Gut Dysbiosis and Immune Dysregulation

doi: 10.1096/fj.202502610R

Figure Lengend Snippet: Impact of periodontitis microbiota on inflammatory factors and T cell subsets in CIA mice. (A) Representative flow cytometry dot plot for Treg cells (CD4 + Foxp3 + ) in mouse spleen. (B) Representative flow cytometry dot plot for Th17 cells (CD4 + IL‐17A + ) in mouse spleen. (C) Comparison of Treg cell proportions in spleen (percentage of CD4 + T cells). (D) Comparison of Th17 cell proportions in spleen. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.) (E) Serum IL‐6 levels in mice. (F) Serum CRP levels in mice. (*: Compared to Con_PBS group, p < 0.05; **: p < 0.01. #: CIA_P group versus CIA_H group, p < 0.05).

Article Snippet: Mouse Treg Cell Staining Kit and Mouse Th17 Cell Staining Kit (Liankebio, Hangzhou, China) were used.

Techniques: Flow Cytometry, Comparison