tgf β2 neutralizing antibody  (R&D Systems)

Journal: Investigative Ophthalmology & Visual Science

Article Title: TXNDC5 in POAG: Promoting Extracellular Matrix Protein Accumulation and Raising Intraocular Pressure

doi: 10.1167/iovs.67.4.46

Figure Lengend Snippet: Proteomic analysis showed that expressions of TXNDC5 and ECM proteins were upregulated in TGFβ2-treated HTM cells. HTM cells were starved with serum-free DMEM/F12 medium for 24 hours and then treated with TGFβ2 (5 ng/mL) for 48 hours. (A) Changes of protein expression after TGFβ2 treatment in HTM cells were represented by volcano plots (≥1.2-fold difference: upregulated genes [ red ] and downregulated genes [ blue ]). Violin plots showed the TGFβ2-induced significant changes in TXNDC5 (B) , LN (C) , FN (D) , COL4A1 (E) , and COL4A2 (F) . ** P < 0.01; *** P < 0.001. (G) KEGG analysis indicated multiple metabolic and signal pathways, including ECM-receptor interaction, were changed markedly.

Article Snippet: Both control and TGFβ2-treated groups were induced with 100 mg/mL CHX (MedChemExpress, Monmouth Junction, NJ, USA) to inhibit protein translation.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: TXNDC5 in POAG: Promoting Extracellular Matrix Protein Accumulation and Raising Intraocular Pressure

doi: 10.1167/iovs.67.4.46

Figure Lengend Snippet: Western blot, qRT-PCR, and immunocytochemistry showed that expressions of TXNDC5 and ECM proteins were upregulated in TGFβ2-treated HTM cells. (A) Concentration of TXNDC5 in the non-POAG ( n = 10) and POAG patients ( n = 7) in aqueous humor detected by ELISA. (B, C) TXNDC5 levels were not correlated with age and IOP in the non-POAG group and in POAG patients ( n = 7–8). (D) Representative images of Western blot analysis showing TGFβ2-induced changes in expressions of TXNDC5 and ECM proteins in HTM cells. (E–H) Quantitative analyses of Western blot of individual protein ( n = 4–6): TXNDC5 ( E ) , LN (F) , FN (G) , Col-IV (H) . Data are presented as mean ± SEM. (A) * P < 0.05 by unpaired, two-tailed t -test compared to non-POAG. (E–H) * P < 0.05; ** P < 0.01; *** P < 0.001 by unpaired, two-tailed t -test compared to control. (I) Representative images of immunofluorescence staining of TXNDC5, LN, FN, and COL-IV proteins in untreated control and TGFβ2-treated groups. Scale bars : 50 µm.

Article Snippet: Both control and TGFβ2-treated groups were induced with 100 mg/mL CHX (MedChemExpress, Monmouth Junction, NJ, USA) to inhibit protein translation.

Techniques: Western Blot, Quantitative RT-PCR, Immunocytochemistry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Control, Immunofluorescence, Staining