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tgf b  (Elabscience Biotechnology)


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    Elabscience Biotechnology tgf b
    Tgf B, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgf+%CE%B21/Mouse+TGF-%CE%B21+(Transforming+Growth+Factor+Beta+1)+ELISA+Kit/pm42857177-158-7-16
    Average 94 stars, based on 49 article reviews
    tgf b - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Methylmalonic acid modulates neutrophil function to promote tumor progression in colorectal cancer
    Article Snippet: Following 48 h incubation, non-invaded cells on the upper membrane surface were removed with cotton swabs and the transmembrane cells were fixed with paraformaldehyde for 30 min, stained with 1% crystalline violet (Sigma-Aldrich, USA) for 20 min, and visualized under a light microscope using the Image J analysis. .. The cell culture supernatant was collected, and the secretion levels of TGF-β1, IL-10, and MPO in each group were quantified using ELISA kits (Elabscience, Cat. No.: E-EL-0162, E-EL-M0046, E-UNEL-H0048). ..

    Article Title: Methylmalonic acid modulates neutrophil function to promote tumor progression in colorectal cancer.
    Article Snippet: Following 48 h incubation, non-invaded cells on the upper membrane surface were removed with cotton swabs and the transmembrane cells were fixed with paraformaldehyde for 30 min, stained with 1% crystalline violet (Sigma-Aldrich, USA) for 20 min, and visualized under a light microscope using the Image J analysis. .. 2.6 Enzyme-linked immunosorbent assay (ELISA) The cell culture supernatant was collected, and the secretion levels of TGF-β1, IL-10, and MPO in each group were quantified using ELISA kits (Elabscience, Cat. No.: EEL-0162, E-EL-M0046, E-UNEL-H0048). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Methylmalonic acid modulates neutrophil function to promote tumor progression in colorectal cancer
    Article Snippet: Following 48 h incubation, non-invaded cells on the upper membrane surface were removed with cotton swabs and the transmembrane cells were fixed with paraformaldehyde for 30 min, stained with 1% crystalline violet (Sigma-Aldrich, USA) for 20 min, and visualized under a light microscope using the Image J analysis. .. The cell culture supernatant was collected, and the secretion levels of TGF-β1, IL-10, and MPO in each group were quantified using ELISA kits (Elabscience, Cat. No.: E-EL-0162, E-EL-M0046, E-UNEL-H0048). ..

    Article Title: Ergotamine modulates CTSK-associated glutamine metabolism and M2 macrophage polarization in hepatocellular carcinoma
    Article Snippet: .. The supernatant was then analyzed for IL-10 (E-EL-H6154), TGF-β1 (E-EL-0162), and IFN-γ (E-EL-H0108) levels using ELISA kits from Elabscience, China. .. We used GraphPad Prism 8 for all statistical analyses.

    Article Title: Intercellular Horizontal Transfer of TXNDC5 mRNA via Extracellular Vesicles Contributes to Tumor‐Associated Macrophage‐Mediated Prostate Cancer Metastasis
    Article Snippet: .. For the measurement of secretory protein concentrations, conditioned medium was collected and centrifuged for 20 min at 10 000 × g, and the supernatant was used to test the concentrations of TGF‐β1 (E‐EL‐0162, Elabscience), CCL22 (E‐EL‐H0029, Elabscience), and VEGFA (E‐EL‐H0111, Elabscience) with ELISA kits according to the manufacturer's instructions. .. The absorbance of the plates was measured at 450 nm wavelength using a microplate reader (Thermo Fisher Scientific).

    Article Title: Intercellular Horizontal Transfer of TXNDC5 mRNA via Extracellular Vesicles Contributes to Tumor-Associated Macrophage-Mediated Prostate Cancer Metastasis.
    Article Snippet: .. For the measurement of secretory protein concentrations, conditioned medium was collected and centrifuged for 20 min at 10 000 × g, and the supernatant was used to test the concentrations of TGF- β1 (E-EL-0162, Elabscience), CCL22 (E-EL-H0029, Elabscience), and VEGFA (E-EL-H0111, Elabscience) with ELISA kits according to the manufacturer’s instructions. .. The absorbance of the plates was measured at 450 nm wavelength using a microplate reader (Thermo Fisher Scientific).

    Article Title: Methylmalonic acid modulates neutrophil function to promote tumor progression in colorectal cancer.
    Article Snippet: Following 48 h incubation, non-invaded cells on the upper membrane surface were removed with cotton swabs and the transmembrane cells were fixed with paraformaldehyde for 30 min, stained with 1% crystalline violet (Sigma-Aldrich, USA) for 20 min, and visualized under a light microscope using the Image J analysis. .. 2.6 Enzyme-linked immunosorbent assay (ELISA) The cell culture supernatant was collected, and the secretion levels of TGF-β1, IL-10, and MPO in each group were quantified using ELISA kits (Elabscience, Cat. No.: EEL-0162, E-EL-M0046, E-UNEL-H0048). ..

    Article Title: Development of a chitosan-hydroxypropyl methylcellulose/nano-fluorapatite hydrogel as a novel biomaterial for enhanced post-tooth extraction bone healing in diabetic conditions
    Article Snippet: .. The levels of TNF-α (Elabscience, Catalog No. E-EL-R2856), IL-1β (Thermo Fisher Scientific, Catalog No. BMS630ALP), TGF-β1 (Elabscience, Catalog No. E-EL-0162), and osteocalcin (Thermo Fisher Scientific, Catalog No. EEL123) were measured via enzyme-linked immunosorbent assay (ELISA) tools following the procedures specified by the manufacturers. .. In addition, the activity of alkaline phosphatase (ALP) was determined using a colorimetric assay (Sigma-Aldrich, USA).



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    Simultaneous application of IL-4 and Arg promotes efferocytosis and improves the pro-regenerative microenvironment (A) Schematic illustration of the in vitro efferocytosis assessment. (B and C) Macrophage efferocytosis assessment via flow cytometry, showing representative scatterplots of macrophages engulfing apoptotic cells (B) and quantification of the efferocytosis rate after 24 h (C). (D) Morphological evaluation of macrophage efferocytosis, showing representative cell tracking images of macrophages (labeled with CMFDA) engulfing apoptotic cells (labeled with CM-Dil) after 24 h of co-culture. (E) Quantification of the efferocytosis rate after 6, 24, and 48 h determined by flow cytometry under Control and IL-4 + Arg conditions. (F) Experimental design for detecting microenvironmental changes following efferocytosis. (G) ROS quantification following efferocytosis. (H) <t>Enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> <t>(ELISA)</t> quantification <t>of</t> <t>TGF-β1</t> and IL-10 in the culture supernatants. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (C, G, and H) or two-way ANOVA followed by Sidak’s multiple comparisons test (E). Sample sizes: n = 3 biological replicates (B–E); n = 5 biological replicates, each performed with 3 technical replicates (G) and n = 3 biological replicates, each performed with 3 technical replicates (H). Scale bars, 100 μm (D) and 20 μm (inset).
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    Simultaneous application of IL-4 and Arg promotes efferocytosis and improves the pro-regenerative microenvironment (A) Schematic illustration of the in vitro efferocytosis assessment. (B and C) Macrophage efferocytosis assessment via flow cytometry, showing representative scatterplots of macrophages engulfing apoptotic cells (B) and quantification of the efferocytosis rate after 24 h (C). (D) Morphological evaluation of macrophage efferocytosis, showing representative cell tracking images of macrophages (labeled with CMFDA) engulfing apoptotic cells (labeled with CM-Dil) after 24 h of co-culture. (E) Quantification of the efferocytosis rate after 6, 24, and 48 h determined by flow cytometry under Control and IL-4 + Arg conditions. (F) Experimental design for detecting microenvironmental changes following efferocytosis. (G) ROS quantification following efferocytosis. (H) <t>Enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> <t>(ELISA)</t> quantification <t>of</t> <t>TGF-β1</t> and IL-10 in the culture supernatants. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (C, G, and H) or two-way ANOVA followed by Sidak’s multiple comparisons test (E). Sample sizes: n = 3 biological replicates (B–E); n = 5 biological replicates, each performed with 3 technical replicates (G) and n = 3 biological replicates, each performed with 3 technical replicates (H). Scale bars, 100 μm (D) and 20 μm (inset).
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    Simultaneous application of IL-4 and Arg promotes efferocytosis and improves the pro-regenerative microenvironment (A) Schematic illustration of the in vitro efferocytosis assessment. (B and C) Macrophage efferocytosis assessment via flow cytometry, showing representative scatterplots of macrophages engulfing apoptotic cells (B) and quantification of the efferocytosis rate after 24 h (C). (D) Morphological evaluation of macrophage efferocytosis, showing representative cell tracking images of macrophages (labeled with CMFDA) engulfing apoptotic cells (labeled with CM-Dil) after 24 h of co-culture. (E) Quantification of the efferocytosis rate after 6, 24, and 48 h determined by flow cytometry under Control and IL-4 + Arg conditions. (F) Experimental design for detecting microenvironmental changes following efferocytosis. (G) ROS quantification following efferocytosis. (H) <t>Enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> <t>(ELISA)</t> quantification <t>of</t> <t>TGF-β1</t> and IL-10 in the culture supernatants. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (C, G, and H) or two-way ANOVA followed by Sidak’s multiple comparisons test (E). Sample sizes: n = 3 biological replicates (B–E); n = 5 biological replicates, each performed with 3 technical replicates (G) and n = 3 biological replicates, each performed with 3 technical replicates (H). Scale bars, 100 μm (D) and 20 μm (inset).
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    Simultaneous application of IL-4 and Arg promotes efferocytosis and improves the pro-regenerative microenvironment (A) Schematic illustration of the in vitro efferocytosis assessment. (B and C) Macrophage efferocytosis assessment via flow cytometry, showing representative scatterplots of macrophages engulfing apoptotic cells (B) and quantification of the efferocytosis rate after 24 h (C). (D) Morphological evaluation of macrophage efferocytosis, showing representative cell tracking images of macrophages (labeled with CMFDA) engulfing apoptotic cells (labeled with CM-Dil) after 24 h of co-culture. (E) Quantification of the efferocytosis rate after 6, 24, and 48 h determined by flow cytometry under Control and IL-4 + Arg conditions. (F) Experimental design for detecting microenvironmental changes following efferocytosis. (G) ROS quantification following efferocytosis. (H) <t>Enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> <t>(ELISA)</t> quantification <t>of</t> <t>TGF-β1</t> and IL-10 in the culture supernatants. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (C, G, and H) or two-way ANOVA followed by Sidak’s multiple comparisons test (E). Sample sizes: n = 3 biological replicates (B–E); n = 5 biological replicates, each performed with 3 technical replicates (G) and n = 3 biological replicates, each performed with 3 technical replicates (H). Scale bars, 100 μm (D) and 20 μm (inset).
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    Image Search Results


    Simultaneous application of IL-4 and Arg promotes efferocytosis and improves the pro-regenerative microenvironment (A) Schematic illustration of the in vitro efferocytosis assessment. (B and C) Macrophage efferocytosis assessment via flow cytometry, showing representative scatterplots of macrophages engulfing apoptotic cells (B) and quantification of the efferocytosis rate after 24 h (C). (D) Morphological evaluation of macrophage efferocytosis, showing representative cell tracking images of macrophages (labeled with CMFDA) engulfing apoptotic cells (labeled with CM-Dil) after 24 h of co-culture. (E) Quantification of the efferocytosis rate after 6, 24, and 48 h determined by flow cytometry under Control and IL-4 + Arg conditions. (F) Experimental design for detecting microenvironmental changes following efferocytosis. (G) ROS quantification following efferocytosis. (H) Enzyme-linked immunosorbent assay (ELISA) quantification of TGF-β1 and IL-10 in the culture supernatants. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (C, G, and H) or two-way ANOVA followed by Sidak’s multiple comparisons test (E). Sample sizes: n = 3 biological replicates (B–E); n = 5 biological replicates, each performed with 3 technical replicates (G) and n = 3 biological replicates, each performed with 3 technical replicates (H). Scale bars, 100 μm (D) and 20 μm (inset).

    Journal: iScience

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment

    doi: 10.1016/j.isci.2026.117739

    Figure Lengend Snippet: Simultaneous application of IL-4 and Arg promotes efferocytosis and improves the pro-regenerative microenvironment (A) Schematic illustration of the in vitro efferocytosis assessment. (B and C) Macrophage efferocytosis assessment via flow cytometry, showing representative scatterplots of macrophages engulfing apoptotic cells (B) and quantification of the efferocytosis rate after 24 h (C). (D) Morphological evaluation of macrophage efferocytosis, showing representative cell tracking images of macrophages (labeled with CMFDA) engulfing apoptotic cells (labeled with CM-Dil) after 24 h of co-culture. (E) Quantification of the efferocytosis rate after 6, 24, and 48 h determined by flow cytometry under Control and IL-4 + Arg conditions. (F) Experimental design for detecting microenvironmental changes following efferocytosis. (G) ROS quantification following efferocytosis. (H) Enzyme-linked immunosorbent assay (ELISA) quantification of TGF-β1 and IL-10 in the culture supernatants. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (C, G, and H) or two-way ANOVA followed by Sidak’s multiple comparisons test (E). Sample sizes: n = 3 biological replicates (B–E); n = 5 biological replicates, each performed with 3 technical replicates (G) and n = 3 biological replicates, each performed with 3 technical replicates (H). Scale bars, 100 μm (D) and 20 μm (inset).

    Article Snippet: Mouse TGF-β1 ELISA Kit , ABclonal , Cat# RK00057.

    Techniques: In Vitro, Flow Cytometry, Cell Tracking Assay, Labeling, Co-culture Assay, Control, Enzyme-linked Immunosorbent Assay

    Transcriptomic changes underlying synergistic regulation of macrophages by IL-4 and Arg (A) RT-qPCR analysis of M2-related marker gene expression ( Tgfb1 , Il10 , Arg1 , and Cd206 ) in macrophages. (B) Schematic illustration of the bone marrow-derived macrophages (BMDMs) isolation, total RNA extraction, and transcriptomic sequencing workflow. (C) Functional annotation of upregulated genes using Gene Ontology (GO) enrichment analysis. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis emphasizing significant signaling pathways. (E) RT-qPCR analysis of Tgfb1 and Il10 mRNA expression levels of macrophage after treatment with the IL-4 + Arg combination in the presence or absence of nor-NOHA. (F) ELISA quantification of secreted TGF-β1 and IL-10 protein concentrations in the culture supernatants under nor-NOHA inhibition. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (A, E, and F). Sample sizes: n = 3 biological replicates (C and D); n = 3 (A and F) and 4 (E) biological replicates, each performed with 3 technical replicates.

    Journal: iScience

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment

    doi: 10.1016/j.isci.2026.117739

    Figure Lengend Snippet: Transcriptomic changes underlying synergistic regulation of macrophages by IL-4 and Arg (A) RT-qPCR analysis of M2-related marker gene expression ( Tgfb1 , Il10 , Arg1 , and Cd206 ) in macrophages. (B) Schematic illustration of the bone marrow-derived macrophages (BMDMs) isolation, total RNA extraction, and transcriptomic sequencing workflow. (C) Functional annotation of upregulated genes using Gene Ontology (GO) enrichment analysis. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis emphasizing significant signaling pathways. (E) RT-qPCR analysis of Tgfb1 and Il10 mRNA expression levels of macrophage after treatment with the IL-4 + Arg combination in the presence or absence of nor-NOHA. (F) ELISA quantification of secreted TGF-β1 and IL-10 protein concentrations in the culture supernatants under nor-NOHA inhibition. Error bars represent SD. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test (A, E, and F). Sample sizes: n = 3 biological replicates (C and D); n = 3 (A and F) and 4 (E) biological replicates, each performed with 3 technical replicates.

    Article Snippet: Mouse TGF-β1 ELISA Kit , ABclonal , Cat# RK00057.

    Techniques: Quantitative RT-PCR, Analysis, Marker, Gene Expression, Derivative Assay, Isolation, RNA Extraction, Sequencing, Functional Assay, Protein-Protein interactions, Expressing, Enzyme-linked Immunosorbent Assay, Inhibition

    Journal: iScience

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment

    doi: 10.1016/j.isci.2026.117739

    Figure Lengend Snippet:

    Article Snippet: Mouse TGF-β1 ELISA Kit , ABclonal , Cat# RK00057.

    Techniques: Cell-Signaling, Recombinant, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Software