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tev protease expression construct  (Addgene inc)


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    Addgene inc tev protease expression construct
    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; <t>+TEV</t> – 2nd Ni-NTA flowthrough collected after <t>TEV</t> <t>protease</t> cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.
    Tev Protease Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tev+protease+expression+construct/TEVSH+(Plasmid+%23125194)/pm37314918-167-1-7
    Average 93 stars, based on 9 article reviews
    tev protease expression construct - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture."

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.

    Journal: STAR protocols

    doi: 10.1016/j.xpro.2023.102351

    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.
    Figure Legend Snippet: Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Techniques Used: SDS Page, Sequencing, Western Blot, Negative Control, Positive Control

    Related Articles

    Expressing:

    Article Title: Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease
    Article Snippet: .. We obtained the TEV protease expression construct from Dr. David S. Waugh via Addgene.org, a nonprofit site dedicated to the dispersal of plasmids to the scientific community. ..

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
    Article Snippet: .. Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid. c. Mix 50 ng of pTEVSH plasmid with ∼50 μL of competent cells. d. Incubate at room temperature for 2 min. e. Add 300 μL of LB broth to mixture. f. Incubate in a shaking incubator at 37°C and ∼250 rpm for 1 h. g. Plate 100 μL onto a pre-warmed LB agar plate containing 100 μg/mL of Ampicillin and 25 μg/mL of chloramphenicol, spread using sterile glass beads. h. Incubate at 37°C for 12–16 h. 42. ..

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.
    Article Snippet: .. Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid. ..

    Article Title: Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease
    Article Snippet: .. TEV protease purification We obtained the TEV protease expression construct from Dr. David S. Waugh via Addgene.org, a nonprofit site dedicated to the dispersal of plasmids to the scientific community. ..

    Construct:

    Article Title: Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease
    Article Snippet: .. We obtained the TEV protease expression construct from Dr. David S. Waugh via Addgene.org, a nonprofit site dedicated to the dispersal of plasmids to the scientific community. ..

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
    Article Snippet: .. Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid. c. Mix 50 ng of pTEVSH plasmid with ∼50 μL of competent cells. d. Incubate at room temperature for 2 min. e. Add 300 μL of LB broth to mixture. f. Incubate in a shaking incubator at 37°C and ∼250 rpm for 1 h. g. Plate 100 μL onto a pre-warmed LB agar plate containing 100 μg/mL of Ampicillin and 25 μg/mL of chloramphenicol, spread using sterile glass beads. h. Incubate at 37°C for 12–16 h. 42. ..

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.
    Article Snippet: .. Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid. ..

    Article Title: Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease
    Article Snippet: .. TEV protease purification We obtained the TEV protease expression construct from Dr. David S. Waugh via Addgene.org, a nonprofit site dedicated to the dispersal of plasmids to the scientific community. ..

    Plasmid Preparation:

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
    Article Snippet: .. Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid. c. Mix 50 ng of pTEVSH plasmid with ∼50 μL of competent cells. d. Incubate at room temperature for 2 min. e. Add 300 μL of LB broth to mixture. f. Incubate in a shaking incubator at 37°C and ∼250 rpm for 1 h. g. Plate 100 μL onto a pre-warmed LB agar plate containing 100 μg/mL of Ampicillin and 25 μg/mL of chloramphenicol, spread using sterile glass beads. h. Incubate at 37°C for 12–16 h. 42. ..

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.
    Article Snippet: .. Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid. ..

    Sterility:

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
    Article Snippet: .. Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid. c. Mix 50 ng of pTEVSH plasmid with ∼50 μL of competent cells. d. Incubate at room temperature for 2 min. e. Add 300 μL of LB broth to mixture. f. Incubate in a shaking incubator at 37°C and ∼250 rpm for 1 h. g. Plate 100 μL onto a pre-warmed LB agar plate containing 100 μg/mL of Ampicillin and 25 μg/mL of chloramphenicol, spread using sterile glass beads. h. Incubate at 37°C for 12–16 h. 42. ..

    Purification:

    Article Title: Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease
    Article Snippet: .. TEV protease purification We obtained the TEV protease expression construct from Dr. David S. Waugh via Addgene.org, a nonprofit site dedicated to the dispersal of plasmids to the scientific community. ..



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    Addgene inc tev protease expression construct
    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; <t>+TEV</t> – 2nd Ni-NTA flowthrough collected after <t>TEV</t> <t>protease</t> cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.
    Tev Protease Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tev+protease+expression+construct/TEVSH+(Plasmid+%23125194)/pm37314918-167-1-7
    Average 93 stars, based on 1 article reviews
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    Image Search Results


    Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Journal: STAR protocols

    Article Title: Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture.

    doi: 10.1016/j.xpro.2023.102351

    Figure Lengend Snippet: Figure 5. Representative results of purification and bioactivity screening (A) SDS-PAGE of FGF2 purification. E – Elution; +TEV – 2nd Ni-NTA flowthrough collected after TEV protease cleavage. The shift in size of the band is due to the removal of the 6His and TEV recognition sequence. (B) Western blot analysis of cell extracts treated with FGF2. C – commercial FGF2; NC – negative control – no FGF2; 1–5 – FGF2 orthologs; PC – positive control – 10% FBS. Panel B was adapted from1.

    Article Snippet: Transform TEV protease expression construct (pTEVSH – Addgene: 125194. ) into BL21 (DE3) RIL competent cells. a. Thaw chemically competent BL21-CodonPlus (DE3)-RIL cells on ice. b. Thaw pTEVSH plasmid.

    Techniques: SDS Page, Sequencing, Western Blot, Negative Control, Positive Control