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tbb  (MedChemExpress)
94
MedChemExpress tbb
A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
Tbb, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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human recombinant ltα  (R&D Systems)
94
R&D Systems human recombinant ltα
A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
Human Recombinant Ltα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human recombinant ltα/product/R&D Systems
Average 94 stars, based on 1 article reviews
human recombinant ltα - by Bioz Stars, 2026-04
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rhlt⍺  (R&D Systems)
94
R&D Systems rhlt⍺
A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
Rhlt⍺, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhlt⍺/product/R&D Systems
Average 94 stars, based on 1 article reviews
rhlt⍺ - by Bioz Stars, 2026-04
94/100 stars
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3 utr  (Addgene inc)
95
Addgene inc 3 utr
A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
3 Utr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
3 utr - by Bioz Stars, 2026-04
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eft 3p  (Addgene inc)
95
Addgene inc eft 3p
A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
Eft 3p, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eft 3p/product/Addgene inc
Average 95 stars, based on 1 article reviews
eft 3p - by Bioz Stars, 2026-04
95/100 stars
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tnfβ  (R&D Systems)
94
R&D Systems tnfβ
A. RCC4 cells were treated with the combination <t>of</t> <t>erastin</t> and <t>TBB</t> for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001
Tnfβ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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A. RCC4 cells were treated with the combination of erastin and TBB for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001

Journal: bioRxiv

Article Title: Identification of 4,5,6,7-Tetrabromo-1 H -benzotriazole (TBB) as a Small Molecule MESH1 Inhibitor that Suppresses Ferroptosis

doi: 10.64898/2026.02.19.706832

Figure Lengend Snippet: A. RCC4 cells were treated with the combination of erastin and TBB for 24hours. Cell viability was measured using CellTiter Glo assay. n=6, error bars = SD. Statistical significance shown at selected erastin doses was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO control vs. TBB concentration . B. Fluorescent microscopy of RCC4 cells incubated with erastin (0.625 µM) and TBB (20 µM) after 24 hours with inclusion of CellTox Green reagent. C . Quantification of CellTox Green fluorescent signal with plate reader of RCC4 cells treated with TBB and erastin (0.625 µM) for 24hours. n=5, error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. erastin + TBB samples. D-E. Measuring lipid peroxidation via C11-BODIPY TM staining of RCC4 cells treated with or without TBB (20 µM) and erastin (5 µM) for 16 hours. Quantification of lipid peroxidation shown in E . n=3 error bars = SD. Statistical significance was determined with One-Way ANOVA with Dunnett’s multiple comparison of erastin vs. TBB and erastin vs erastin + TBB samples F. Measurement of GSH/GSSG ratio of RCC4 cells treated with or without erastin (1.25 µM) and with or without TBB (20 µM) for 6 hours. n=4 error bars = SEM. Statistical significance was determined with Two-way ANOVA with Sidák multiple comparisons (TBB vs. DMSO) G. Representative immunoblot showing soluble MESH1 and β-tubulin after treating RCC4 cells with a serial dilution of TBB concentrations (20 µM-1.25 µM) or DMSO control and heating the cells to 50 ℃ for 3 min. H. Quantification of relative fold increase of soluble MESH1. The MESH1 band intensity was normalized to β-tubulin loading control and then normalized to DMSO. n=3 independent experiments. Statistical Significance was assessed with One-Way Anova with Dunnett’s multiple comparisons test for comparing each TBB concentration to DMSO. I. Viability determined with CellTiter Glo of RCC4 cells transfected with siNT, siCK2⍺, or siMESH1 and treated with 10 µM erastin and DMSO or 10 µM TBB for 24 hours. n=6, error bars = SD. Statistical significance was determined using Two-Way ANOVA with Dunnett’s multiple comparison of DMSO vs. TBB. J. Representative images of neurons transfected with YFP+ or Tau and treated with TBB. K . Quantification of neuron survival. One-way ANOVA with Dunnett’s post-hoc test vs. Tau 4R. * = p-value <0.05, ** = p-value < 0.01, *** = p value <0.001, **** p-value < 0.0001

Article Snippet: Erastin (MedChem Express:HY-15763), IKE (MedChem Express: HY-114481), TBB (MedChem Express: HY-14394), TFBt (Enamine; EN300-6487228), TCBt (Sigma; COMH04236427), TIBi (AABlocks; AA01X6YQ), 5-monoBBt (Enamine; EN300-137666), 4-monoBBt (Enamine; EN300-137659), 5,6-diBBt (Enamine; EN300-6775516), 5-monoIBt (Enamine; EN300-2951206), 4-F,5-monoBBt (Enamine; EN300-5495791), 6,7diBQX (Enamine; EN300-19483685), DMAT (MedChem Express: HY-15535), TBCA (MedChem Express: HY-110052), DRB (MedChem Express: HY-14392), TBBi (Cayman Chemical, 18886), DPPH (Thermo Scientific, 044150.MD), Trolox (Thermo Scientific, 218940010), Ferrozine (Sigma, 160601), DFO (Sigma, D9533)

Techniques: Glo Assay, Comparison, Control, Concentration Assay, Microscopy, Incubation, CellTox Assay, Staining, Western Blot, Serial Dilution, Transfection