Journal: Bone Research
Article Title: The endosteal niche regulates breast cancer cell dormancy in bone: identification of new molecular determinants
doi: 10.1038/s41413-026-00535-3
Figure Lengend Snippet: Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human T47D and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
Article Snippet: The human BrCa cell lines MDA-MB231, T47D, ZR75D, BT474, and the murine BrCa cell line 4T1 were purchased from the American Type Culture Collection (ATCC) and cultured according to the supplier instruction.
Techniques: Expressing, Flow Cytometry, Membrane