Incubation:Article Title: Reduced Interaction of VAMP2 and Aggregated α-synuclein by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson’s Disease.
Article Snippet: Blots were blocked for one hour in Tris-buffered saline (TBS) (10 mM Tris–HCl, pH 7.5, 150 mM NaCl) containing 5% nonfat dry milk (BioRad, Berkeley, CA, USA) at room temperature and washed three times with TBS. .. Blots were then incubated at 4 °C overnight with the following antibodies: synaptosomal-associated protein, 25 kDa (SNAP-25, 1:1,000, Abcam, Cambridge, UK); syntaxin1 (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA); vesicle-associated membrane protein 2 (VAMP2, 1:1,000, Synaptic systems, Goettingen, Germany); dopamine transporter (DAT, 1:1,000, Chemicon, Pemecula, CA, USA); dopamine receptor D1 (DRD1, Page 6/20 1:1,000, Proteintech, Manchester, UK); dopamine receptor D2 (DRD2, 1:1,000, Abcam, Cambridge, UK); human α-Syn (1:1,000, Abcam, Cambridge, UK); human pSer129 α-Syn (1:1,000, Abcam, Cambridge, UK); and tyrosine hydroxylase (TH, 1:1,000, Sigma Aldrich, St Louis, MO, USA) in TBST (10 mM Tris pH 7.5, 150 mM NaCl, and 0.02% Tween 20) with 3% nonfat dry milk. .. After incubation, the blots were rinsed three times with TBST and incubated for one hour with horseradish peroxidase-conjugated secondary antibodies (1:3,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA) at room temperature.
Article Title: NMDA Receptor Hypofunction Induces Dysfunctions of Energy Metabolism And Semaphorin Signaling in Rats: A Synaptic Proteome Study
Article Snippet: .. The membranes were incubated at 4°C overnight with monoclonal antibody against Synaptophysin (sc-55507; Santa Cruz Biotechnology, Paso Robles, California), GluR1 (sc-55509; Santa Cruz Biotechnology), Syntaxin1 (sc-12736; Santa Cruz Biotechnology), and Tpi1 (sc-166785; Santa Cruz Biotechnology), followed by species-specific horseradish peroxidase-conjugated secondary antibody for 2 hours at room temperature. .. Blots were finally incubated with ECL chemiluminescent reagent (Santa Cruz Biotechnology, Paso Robles, California) and exposed to X-ray film.
Article Title: Reduced Interaction of Aggregated α-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson’s Disease
Article Snippet: The blots were blocked for 1 h in Tris-buffered saline (TBS) (10 mM Tris-HCl, pH 7.5, 150 mM NaCl) containing 5% nonfat dry milk (Bio-Rad, 232100, Berkeley, CA, USA) at 25 °C and washed three times with TBS. .. Then, the blots were incubated at 4 °C overnight with the following antibodies: antibodies against synaptosomal-associated protein, 25 kDa (SNAP-25, 1:1000, Abcam, ab5666, Cambridge, UK); syntaxin1 (1:1000, Santa Cruz Biotechnology, Santa Cruz, sc-12736, CA, USA); VAMP2 (1:1000, Abcam, ab181869, Cambridge, UK); solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3, 1:1000, Chemicon, AMT-003, Pemecula, CA, USA); dopamine receptor D1 (Drd1, 1:1000, Proteintech, 17934-1-AP, Manchester, UK); Drd2 (1:1000, Abcam, ab32349, Cambridge, UK); α-Syn (1:1000, Abcam, ab138501, Cambridge, UK); human pSer129 α-Syn (1:1000, Abcam, ab168381, Cambridge, UK); and tyrosine hydroxylase (TH, 1:1000, Sigma Aldrich, T1299, St. Louis, MO, USA) diluted in Tris-buffered Saline, 0.1% TWEEN® 20 (10 mM Tris pH 7.5, 150 mM NaCl, and 0.02% Tween 20) with 3% nonfat dry milk. .. After incubation, the blots were rinsed three times with TBST and incubated for 1 h with horseradish peroxidase-conjugated secondary antibodies (1:3000, Santa Cruz Biotechnology, anti-rabbit; sc-2357, anti-mouse; sc-516102, Santa Cruz, CA, USA) at room temperature.
Article Title: Reduced Interaction of Aggregated α-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson's Disease.
Article Snippet: The blots were blocked for 1 h in Tris-buffered saline (TBS) (10 mM Tris-HCl, pH 7.5, 150 mM NaCl) containing 5% nonfat dry milk (Bio-Rad, 232100, Berkeley, CA, USA) at 25 ◦C and washed three times with TBS. .. Then, the blots were incubated at 4 ◦C overnight with the following antibodies: antibodies against synaptosomal-associated protein, 25 kDa (SNAP-25, 1:1000, Abcam, ab5666, Cambridge, UK); syntaxin1 (1:1000, Santa Cruz Biotechnology, Santa Cruz, sc-12736, CA, USA); VAMP2 (1:1000, Abcam, ab181869, Cambridge, UK); solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3, 1:1000, Chemicon, AMT-003, Pemecula, CA, USA); dopamine receptor D1 (Drd1, 1:1000, Proteintech, 17934-1-AP, Manchester, UK); Drd2 (1:1000, Abcam, ab32349, Cambridge, UK); α-Syn (1:1000, Abcam, ab138501, Cambridge, UK); human pSer129 α-Syn (1:1000, Abcam, ab168381, Cambridge, UK); and tyrosine hydroxylase (TH, 1:1000, Sigma Aldrich, T1299, St. Louis, MO, USA) diluted in Tris-buffered Saline, 0.1% TWEEN® 20 (10 mM Tris pH 7.5, 150 mM NaCl, and 0.02% Tween 20) with 3% nonfat dry milk. .. After incubation, the blots were rinsed three times with TBST and incubated for 1 h with horseradish peroxidase-conjugated secondary antibodies (1:3000, Santa Cruz Biotechnology, anti-rabbit; sc-2357, anti-mouse; sc-516102, Santa Cruz, CA, USA) at room temperature.
Membrane:Article Title: Reduced Interaction of VAMP2 and Aggregated α-synuclein by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson’s Disease.
Article Snippet: Blots were blocked for one hour in Tris-buffered saline (TBS) (10 mM Tris–HCl, pH 7.5, 150 mM NaCl) containing 5% nonfat dry milk (BioRad, Berkeley, CA, USA) at room temperature and washed three times with TBS. .. Blots were then incubated at 4 °C overnight with the following antibodies: synaptosomal-associated protein, 25 kDa (SNAP-25, 1:1,000, Abcam, Cambridge, UK); syntaxin1 (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA); vesicle-associated membrane protein 2 (VAMP2, 1:1,000, Synaptic systems, Goettingen, Germany); dopamine transporter (DAT, 1:1,000, Chemicon, Pemecula, CA, USA); dopamine receptor D1 (DRD1, Page 6/20 1:1,000, Proteintech, Manchester, UK); dopamine receptor D2 (DRD2, 1:1,000, Abcam, Cambridge, UK); human α-Syn (1:1,000, Abcam, Cambridge, UK); human pSer129 α-Syn (1:1,000, Abcam, Cambridge, UK); and tyrosine hydroxylase (TH, 1:1,000, Sigma Aldrich, St Louis, MO, USA) in TBST (10 mM Tris pH 7.5, 150 mM NaCl, and 0.02% Tween 20) with 3% nonfat dry milk. .. After incubation, the blots were rinsed three times with TBST and incubated for one hour with horseradish peroxidase-conjugated secondary antibodies (1:3,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA) at room temperature.
Saline:Article Title: Reduced Interaction of Aggregated α-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson’s Disease
Article Snippet: The blots were blocked for 1 h in Tris-buffered saline (TBS) (10 mM Tris-HCl, pH 7.5, 150 mM NaCl) containing 5% nonfat dry milk (Bio-Rad, 232100, Berkeley, CA, USA) at 25 °C and washed three times with TBS. .. Then, the blots were incubated at 4 °C overnight with the following antibodies: antibodies against synaptosomal-associated protein, 25 kDa (SNAP-25, 1:1000, Abcam, ab5666, Cambridge, UK); syntaxin1 (1:1000, Santa Cruz Biotechnology, Santa Cruz, sc-12736, CA, USA); VAMP2 (1:1000, Abcam, ab181869, Cambridge, UK); solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3, 1:1000, Chemicon, AMT-003, Pemecula, CA, USA); dopamine receptor D1 (Drd1, 1:1000, Proteintech, 17934-1-AP, Manchester, UK); Drd2 (1:1000, Abcam, ab32349, Cambridge, UK); α-Syn (1:1000, Abcam, ab138501, Cambridge, UK); human pSer129 α-Syn (1:1000, Abcam, ab168381, Cambridge, UK); and tyrosine hydroxylase (TH, 1:1000, Sigma Aldrich, T1299, St. Louis, MO, USA) diluted in Tris-buffered Saline, 0.1% TWEEN® 20 (10 mM Tris pH 7.5, 150 mM NaCl, and 0.02% Tween 20) with 3% nonfat dry milk. .. After incubation, the blots were rinsed three times with TBST and incubated for 1 h with horseradish peroxidase-conjugated secondary antibodies (1:3000, Santa Cruz Biotechnology, anti-rabbit; sc-2357, anti-mouse; sc-516102, Santa Cruz, CA, USA) at room temperature.
Article Title: Reduced Interaction of Aggregated α-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson's Disease.
Article Snippet: The blots were blocked for 1 h in Tris-buffered saline (TBS) (10 mM Tris-HCl, pH 7.5, 150 mM NaCl) containing 5% nonfat dry milk (Bio-Rad, 232100, Berkeley, CA, USA) at 25 ◦C and washed three times with TBS. .. Then, the blots were incubated at 4 ◦C overnight with the following antibodies: antibodies against synaptosomal-associated protein, 25 kDa (SNAP-25, 1:1000, Abcam, ab5666, Cambridge, UK); syntaxin1 (1:1000, Santa Cruz Biotechnology, Santa Cruz, sc-12736, CA, USA); VAMP2 (1:1000, Abcam, ab181869, Cambridge, UK); solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3, 1:1000, Chemicon, AMT-003, Pemecula, CA, USA); dopamine receptor D1 (Drd1, 1:1000, Proteintech, 17934-1-AP, Manchester, UK); Drd2 (1:1000, Abcam, ab32349, Cambridge, UK); α-Syn (1:1000, Abcam, ab138501, Cambridge, UK); human pSer129 α-Syn (1:1000, Abcam, ab168381, Cambridge, UK); and tyrosine hydroxylase (TH, 1:1000, Sigma Aldrich, T1299, St. Louis, MO, USA) diluted in Tris-buffered Saline, 0.1% TWEEN® 20 (10 mM Tris pH 7.5, 150 mM NaCl, and 0.02% Tween 20) with 3% nonfat dry milk. .. After incubation, the blots were rinsed three times with TBST and incubated for 1 h with horseradish peroxidase-conjugated secondary antibodies (1:3000, Santa Cruz Biotechnology, anti-rabbit; sc-2357, anti-mouse; sc-516102, Santa Cruz, CA, USA) at room temperature.
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