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synaptosome isolation kit  (Invent Biotechnologies)


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    Structured Review

    Invent Biotechnologies synaptosome isolation kit
    Synaptosome Isolation Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/synaptosome+isolation+kit/MinuteTM+Synaptosome+Isolation+Kit/pm41843582-235-7-10
    Average 94 stars, based on 13 article reviews
    synaptosome isolation kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes
    Article Snippet: .. Tissues were immediately processed using the Minute Synaptosome Isolation Kit (Invent Biotechnologies, Plymouth, MN, USA) for synaptosome fraction isolation. ..

    Article Title: Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics
    Article Snippet: .. Hippocampal synaptosomes (containing synaptic mitochondria) were isolated using the MinuteTM Synaptosome Isolation Kit (SY-052, Invent Biotechnologies, China). ..

    Article Title: Contributions of synaptic energetic dysfunction by microtubule dynamics and microtubule-based mitochondrial transport disorder to morphine tolerance.
    Article Snippet: .. Spinal synaptosomes (containing synaptic mitochondria) were isolated using the MinuteTM Synaptosome Isolation Kit (Cat. No. SY-052, Invent Biotechnologies, Plymouth, MN, USA) (Han et al., 2024). ..

    Article Title: ALS-associated RNA-binding proteins promote UNC13A transcription through REST downregulation
    Article Snippet: To overcome this limitation, we attempted to induce synapse formation by differentiating the cells using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF) (Forster JI. et al., J Biomol Screen, 2016). .. Following RA-BDNF differentiation, we performed synaptosome isolation using a synaptosome isolation kit (Invent Biotechnologies, Inc.), as the referee suggested, to determine whether synapses had formed. ..

    Article Title: Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics
    Article Snippet: .. Scale bar is 50 μm. (C) Schematic diagram of procedures for synaptosomal isolation by differential centrifugaion using a synaptosome isolation kit (Invent Biotechnologies, Beijing, China). (D) WB was used to authenticate the isolation efficiency of synaptosomes. (E) Expressions of the mitochondrial dynamic-related proteins, OPA1, MFN1/2, pDRP1 (Ser616) and FIS1 within synaptosomes isolated from the hippocampus of control and DE rats as evaluated using WB, and analysis of WB with HSP60 as the mitochondrial internal reference (n = 6). ..

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes.
    Article Snippet: .. Tissues were immediately processed using the Minute Synaptosome Isolation Kit (Invent Biotechnologies, Plymouth, MN, USA) for synaptosome fraction isolation. ..

    Article Title: Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics.
    Article Snippet: .. Hippocampal synaptosomes (containing synaptic mitochondria) were isolated using the MinuteTM Synaptosome Isolation Kit (SY-052, Invent Biotechnologies, China). ..

    Article Title: Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics.
    Article Snippet: .. To further investigate mitochondrial function within synapses, we isolated hippocampal synaptosomes, which containing synaptic mitochondria, as achieved using a synaptosome isolation kit (Invent Biotechnologies, Beijing, China). ..

    Western Blot:

    Article Title: Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics
    Article Snippet: .. Scale bar is 50 μm. (C) Schematic diagram of procedures for synaptosomal isolation by differential centrifugaion using a synaptosome isolation kit (Invent Biotechnologies, Beijing, China). (D) WB was used to authenticate the isolation efficiency of synaptosomes. (E) Expressions of the mitochondrial dynamic-related proteins, OPA1, MFN1/2, pDRP1 (Ser616) and FIS1 within synaptosomes isolated from the hippocampus of control and DE rats as evaluated using WB, and analysis of WB with HSP60 as the mitochondrial internal reference (n = 6). ..

    Control:

    Article Title: Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics
    Article Snippet: .. Scale bar is 50 μm. (C) Schematic diagram of procedures for synaptosomal isolation by differential centrifugaion using a synaptosome isolation kit (Invent Biotechnologies, Beijing, China). (D) WB was used to authenticate the isolation efficiency of synaptosomes. (E) Expressions of the mitochondrial dynamic-related proteins, OPA1, MFN1/2, pDRP1 (Ser616) and FIS1 within synaptosomes isolated from the hippocampus of control and DE rats as evaluated using WB, and analysis of WB with HSP60 as the mitochondrial internal reference (n = 6). ..



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    VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the <t>synaptosomes</t> of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.
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    Invent Biotechnologies sy 052
    VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the <t>synaptosomes</t> of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.
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    Image Search Results


    VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the synaptosomes of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.

    Journal: Pharmacology Research & Perspectives

    Article Title: HDAC4 /5 Inhibitor, LMK ‐235 Improves Animal Voluntary Movement in MPTP ‐Induced Parkinson's Disease Model

    doi: 10.1002/prp2.70057

    Figure Lengend Snippet: VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the synaptosomes of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.

    Article Snippet: Synaptosomes were isolated using a Minute Synaptosome Isolation Kit (SY‐052; Invitrogen, Waltham, MA, USA), and the DA concentration in the cytosol or synaptosomes was measured using a DA ELISA kit (ab285238; Abcam).

    Techniques: Expressing, Concentration Assay, Inhibition, Control